公开(公告)号：US20150093790A1

公开(公告)日：2015-04-02

申请号：US13997170

申请日：2011-12-20

申请人： The Board of TRustees of the UNoiiversuty of Illinois

发明人： Yejun Han ,  Xiaoyun Su ,  Dylan Dodd ,  Roderick I. Mackie ,  Issac K.O. Cann

IPC分类号： C12P19/14 ,  C12N9/18 ,  C12P19/02 ,  C12P7/04 ,  C12P7/16 ,  C12N9/24 ,  C12P7/14

CPC分类号： C12P19/14 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2405 ,  C12N9/2434 ,  C12N9/248 ,  C12N9/2482 ,  C12P7/04 ,  C12P7/14 ,  C12P7/16 ,  C12P19/02 ,  C12Y301/01072 ,  C12Y302/01008 ,  C12Y302/01055 ,  C12Y302/01072 ,  C12Y302/01139

摘要： The disclosure provides thermostable enzymes isolated from Caldicellulosiruptor bescii and fragments thereof useful for the degradation of cellulose and/or hemicellulose, including thermostable cellulases and hemicellulases. The disclosure further provides nucleic acids encoding the thermostable enzymes of the disclosure. The disclosure also provides methods for the conversion of cellulose and hemicellulose into fermentable sugars using thermostable enzymes of the disclosure. The disclosure also provides enzyme cocktails containing multiple enzymes disclosed herein. The enzymes can be used to release sugars present in cellulose or hemicellulose for subsequent fermentation to produce value-added products.

摘要翻译： 本公开提供从Caldicellulosiruptor bescii分离的热稳定酶及其片段，其可用于纤维素和/或半纤维素（包括耐热纤维素酶和半纤维素酶）的降解。 本公开进一步提供了编码本公开的热稳定酶的核酸。 本公开还提供了使用本公开的热稳定酶将纤维素和半纤维素转化为可发酵糖的方法。 本公开还提供含有本文公开的多种酶的酶混合物。 酶可用于释放纤维素或半纤维素中存在的糖，用于随后的发酵以产生增值产物。