公开(公告)号：US20150259656A1

公开(公告)日：2015-09-17

申请号：US14428246

申请日：2013-09-16

申请人： TECHNISCHE UNIVERSITÄT WIEN

发明人： Christian Kubicek ,  Razieh Karimi

IPC分类号： C12N9/24

CPC分类号： C12N9/2402 ,  C07K14/37 ,  C12N1/14 ,  C12P21/00 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01008 ,  C12Y302/01014 ,  C12Y302/01022 ,  C12Y302/01055 ,  C12Y302/01072

摘要： The present invention relates to a genetically modified fungal cell of the genus Trichoderma capable to express the regulatory protein VEL1 in a higher amount compared to the unmodified wild type fungal cell.

摘要翻译： 本发明涉及与未修饰的野生型真菌细胞相比能够表达更高量的调节蛋白VEL1的木霉属的遗传修饰真菌细胞。

公开(公告)号：US20150225705A1

公开(公告)日：2015-08-13

申请号：US14424560

申请日：2013-03-15

申请人： Lallemand Hungary Liquidity Management LLC

发明人： John E. McBride ,  Erin Wiswall ,  Indraneel Shikhare ,  Haowen Xu ,  Naomi Thorngren ,  Heidi H. Hau ,  Emily Stonehouse

IPC分类号： C12N9/18 ,  C12N9/40 ,  C12N9/42 ,  C12P7/10 ,  C12N9/38 ,  C12P3/00 ,  C12P7/52 ,  C12P7/28 ,  C12P7/16 ,  C12N9/24 ,  C12P7/56

CPC分类号： C12N9/18 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2491 ,  C12N9/2494 ,  C12P3/00 ,  C12P7/10 ,  C12P7/16 ,  C12P7/28 ,  C12P7/52 ,  C12P7/56 ,  C12P19/02 ,  C12P19/14 ,  C12Y301/01006 ,  C12Y301/01072 ,  C12Y302/01021 ,  C12Y302/01037 ,  C12Y302/01072 ,  C12Y302/01131 ,  C12Y302/01139 ,  Y02E50/16

摘要： The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.

摘要翻译： 本发明提供了一种多组分酶系统，其从硬木中水解半纤维素寡聚体，其可以在例如酿酒酵母（Saccharomyces cerevisiae）的酵母中表达。 在一些实施方案中，本发明提供一系列生物催化剂的工程化，其将该酶系统的组分的表达和分泌与强大的，快速的木糖利用和在工业上相关的工艺条件下的乙醇发酵相结合，用于固结的生物处理。 在一些实施方案中，本发明利用菌株的共培养物，其可以通过在发酵系统中引入另外的酶而实现显着改善的性能。

公开(公告)号：US20190119687A1

公开(公告)日：2019-04-25

申请号：US16157692

申请日：2018-10-11

申请人： Dongguan APAC Biotechnology CO., Ltd.

发明人： Ya-Shan Cheng ,  Tzu-Hui Wu ,  Cheng-Yen Lin ,  Hui-Lin Lai ,  Cheng-Bin Zheng ,  Ting-Yung Huang ,  I-Hsuan Lin ,  Jian-Wen Huang ,  Chun-Chi Chen ,  Rey-Ting Guo

IPC分类号： C12N15/52 ,  C12N9/24 ,  C12N15/10

CPC分类号： C12N15/52 ,  C12N9/2402 ,  C12N15/102 ,  C12Y302/01037 ,  C12Y302/01072 ,  C12Y302/01177

摘要： A xylosidase having improved enzymatic activity is disclosed. The amino acid sequence of the xylosidase is a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of phenylalanine at position 35 with glutamate, and/or a substitution of glutamine at position 41 with histidine.

公开(公告)号：US09765371B2

公开(公告)日：2017-09-19

申请号：US13997170

申请日：2011-12-20

申请人： Yejun Han ,  Xiaoyun Su ,  Dylan Dodd ,  Roderick I. Mackie ,  Isaac K. O. Cann

发明人： Yejun Han ,  Xiaoyun Su ,  Dylan Dodd ,  Roderick I. Mackie ,  Isaac K. O. Cann

IPC分类号： C12P19/14 ,  C12P7/04 ,  C12P7/16 ,  C12N9/18 ,  C12N9/26 ,  C12N9/42 ,  C12N1/20 ,  C12P21/06 ,  C12P19/34 ,  C12N9/24 ,  C12P7/14 ,  C12P19/02

CPC分类号： C12P19/14 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2405 ,  C12N9/2434 ,  C12N9/248 ,  C12N9/2482 ,  C12P7/04 ,  C12P7/14 ,  C12P7/16 ,  C12P19/02 ,  C12Y301/01072 ,  C12Y302/01008 ,  C12Y302/01055 ,  C12Y302/01072 ,  C12Y302/01139

摘要： The disclosure provides thermostable enzymes isolated from Caldicellulosiruptor bescii and fragments thereof useful for the degradation of cellulose and/or hemicellulose, including thermostable cellulases and hemicellulases. The disclosure further provides nucleic acids encoding the thermostable enzymes of the disclosure. The disclosure also provides methods for the conversion of cellulose and hemicellulose into fermentable sugars using thermostable enzymes of the disclosure. The disclosure also provides enzyme cocktails containing multiple enzymes disclosed herein. The enzymes can be used to release sugars present in cellulose or hemicellulose for subsequent fermentation to produce value-added products.

公开(公告)号：US09745560B2

公开(公告)日：2017-08-29

申请号：US14424560

申请日：2013-03-15

申请人： Lallemand Hungary Liquidity Management LLC

发明人： John E. McBride ,  Erin Wiswall ,  Indraneel Shikhare ,  Haowen Xu ,  Naomi Thorngren ,  Heidi H. Hau ,  Emily Stonehouse

IPC分类号： C12N1/20 ,  C12N9/18 ,  C12P19/02 ,  C12P19/14 ,  C12N9/24 ,  C12P7/10 ,  C12N9/42 ,  C12N9/40 ,  C12N9/38 ,  C12P3/00 ,  C12P7/16 ,  C12P7/28 ,  C12P7/52 ,  C12P7/56

CPC分类号： C12N9/18 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2491 ,  C12N9/2494 ,  C12P3/00 ,  C12P7/10 ,  C12P7/16 ,  C12P7/28 ,  C12P7/52 ,  C12P7/56 ,  C12P19/02 ,  C12P19/14 ,  C12Y301/01006 ,  C12Y301/01072 ,  C12Y302/01021 ,  C12Y302/01037 ,  C12Y302/01072 ,  C12Y302/01131 ,  C12Y302/01139 ,  Y02E50/16

摘要： The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.

公开(公告)号：US20150093790A1

公开(公告)日：2015-04-02

申请号：US13997170

申请日：2011-12-20

申请人： The Board of TRustees of the UNoiiversuty of Illinois

发明人： Yejun Han ,  Xiaoyun Su ,  Dylan Dodd ,  Roderick I. Mackie ,  Issac K.O. Cann

IPC分类号： C12P19/14 ,  C12N9/18 ,  C12P19/02 ,  C12P7/04 ,  C12P7/16 ,  C12N9/24 ,  C12P7/14

CPC分类号： C12P19/14 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2405 ,  C12N9/2434 ,  C12N9/248 ,  C12N9/2482 ,  C12P7/04 ,  C12P7/14 ,  C12P7/16 ,  C12P19/02 ,  C12Y301/01072 ,  C12Y302/01008 ,  C12Y302/01055 ,  C12Y302/01072 ,  C12Y302/01139

摘要： The disclosure provides thermostable enzymes isolated from Caldicellulosiruptor bescii and fragments thereof useful for the degradation of cellulose and/or hemicellulose, including thermostable cellulases and hemicellulases. The disclosure further provides nucleic acids encoding the thermostable enzymes of the disclosure. The disclosure also provides methods for the conversion of cellulose and hemicellulose into fermentable sugars using thermostable enzymes of the disclosure. The disclosure also provides enzyme cocktails containing multiple enzymes disclosed herein. The enzymes can be used to release sugars present in cellulose or hemicellulose for subsequent fermentation to produce value-added products.

摘要翻译： 本公开提供从Caldicellulosiruptor bescii分离的热稳定酶及其片段，其可用于纤维素和/或半纤维素（包括耐热纤维素酶和半纤维素酶）的降解。 本公开进一步提供了编码本公开的热稳定酶的核酸。 本公开还提供了使用本公开的热稳定酶将纤维素和半纤维素转化为可发酵糖的方法。 本公开还提供含有本文公开的多种酶的酶混合物。 酶可用于释放纤维素或半纤维素中存在的糖，用于随后的发酵以产生增值产物。

公开(公告)号：US5932452A

公开(公告)日：1999-08-03

申请号：US289803

申请日：1994-08-12

申请人： Annikka Mustranta ,  Kaisa Poutanen ,  Heikki Heikkila ,  Marja-Leena Sarkki

发明人： Annikka Mustranta ,  Kaisa Poutanen ,  Heikki Heikkila ,  Marja-Leena Sarkki

IPC分类号： C12N11/08 ,  C12N9/24 ,  C12N11/02 ,  C12P19/02 ,  C12P19/14

CPC分类号： C12Y302/01032 ,  C12N11/02 ,  C12N9/248 ,  C12P19/02 ,  C12Y302/01008 ,  C12Y302/01037 ,  C12Y302/01072

摘要： The invention relates to a process for the hydrolysis of a hemicellulose substrate containing xylo-oligomers with an immobilized enzyme by adjusting the pH of an aqueous solution containing hemicellulose oligosaccharides and optionally ionic components to a value within a weakly acidic range, and contacting the solution with a hemicellulolytic enzyme or mixture of enzymes immobilized on a regenerable weak cation exchange carrier without fixing additives, and recovering xylose from the solution. The invention also concerns an enzyme preparation to be used in the process, comprising a weak cation exchange carrier and a hemicellulolytic enzyme or mixture of enzymes immobilized on said carrier.

摘要翻译： PCT No.PCT / FI90 / 00206 Sec。 371日期：1992年3月27日 102（e）日期1992年3月27日PCT提交1990年9月21日PCT公布。 出版物WO91 / 03566 日期1991年3月21日本发明涉及通过将含有半纤维素寡糖和任选的离子组分的水溶液的pH调节至弱酸性范围内的值来水解具有固定化酶的含有低聚木糖的半纤维素底物的方法， 并将溶液与固定在可再生的弱阳离子交换载体上的半纤维素分解酶或酶的混合物接触而不固定添加剂，并从溶液中回收木糖。 本发明还涉及在该方法中使用的酶制剂，其包含弱阳离子交换载体和半纤维素分解酶或固定在所述载体上的酶的混合物。

公开(公告)号：US5432074A

公开(公告)日：1995-07-11

申请号：US75516

申请日：1993-08-13

申请人： Christopher T. Evans ,  Stephen P. Mann ,  Robert C. Charley ,  David Parfitt

发明人： Christopher T. Evans ,  Stephen P. Mann ,  Robert C. Charley ,  David Parfitt

IPC分类号： A23B4/00 ,  A23K1/165 ,  A23K3/03 ,  A23B7/10 ,  C12N9/24 ,  C12N9/26 ,  C12N9/38

CPC分类号： C12Y301/01011 ,  A23K10/14 ,  A23K30/18 ,  C12Y302/01001 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/01023 ,  C12Y302/01031 ,  C12Y302/01032 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01072 ,  C12Y302/01088 ,  C12Y302/01089 ,  C12Y402/0201

摘要： A formulation consisting essentially of -1,4-xylanase and -1,3-xylosidase, but essentially free of -1,4-glucanase and -1,4-cellobiohydrolase and on or more lactic acid-producing bacteria is disclosed. The formulation can be used in a silage composition to stabilize silage from cereal and other crops and to enhance its nutritive value and digestibility in a ruminant or other animal.

摘要翻译： PCT No.PCT / GB91 / 02246 Sec。 371日期：1993年8月13日 102（e）日期1993年8月13日PCT 1991年12月16日PCT PCT。 第WO92 / 10945号公报 日本1992年7月9日。基本上由-1,4-木聚糖酶和1,3-木糖苷酶组成的制剂，但基本上不含-1,4-葡聚糖酶和-1,4-纤维二糖水解酶，以及一种或多种乳酸 - 公开了生产细菌。 该制剂可用于青贮饲料组合物以稳定来自谷物和其他作物的青贮饲料，并增强其在反刍动物或其他动物中的营养价值和消化率。