公开(公告)号：US20220193029A1

公开(公告)日：2022-06-23

申请号：US17599207

申请日：2020-05-19

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Paul Wender ,  Akira J. Shimizu ,  Clayton Hardman

IPC: A61K31/365

Abstract: Provided herein is the use of bryostatin agents to selectively enhance expression, translocation and/or cell surface presentation of an antigen in target cells of interest to modulate immunogenicity of the target cells. Aspects of the methods include, administering an effective amount of a bryostatin agent to a subject to modulate immunogenicity of target cells. The subject methods include a method of treating cancer, including administering to a subject an effective amount of a bryostatin agent to enhance cell surface antigen or neoantigen presentation on target cells of the subject, and administering to the subject a therapeutically effective amount of a therapeutic agent that specifically binds the cell surface antigen to treat the subject for cancer. Aspects of the subject methods also include use of the bryostatin agents to sensitize the target cells to clearance by the subject's immune system.

公开(公告)号：US20210292309A1

公开(公告)日：2021-09-23

申请号：US17162554

申请日：2021-01-29

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Paul Wender ,  Ryan Quiroz ,  Stephen Ho ,  Akira Shimizu ,  Steven Ryckbosch ,  Matthew C. Stevens ,  Matthew S. Jeffreys ,  Clayton Hardman ,  Jack Sloane

IPC: C07D407/14 ,  A61K31/351 ,  A61P25/28 ,  A61P31/18 ,  C07D493/22 ,  A61P35/00 ,  A61K31/365

Abstract: Methods for preparing a variety of bryostatin compounds are provided. The subject methods provide for preparation of bryostatin 1 in multi-gram quantities in a low and unprecedented number of convergent synthetic steps from commercially available materials. The subject methods are scalable with low estimated material costs and can provide enough material to meet clinical needs. Also provided are a variety of bryostatin analog compounds, and prodrug forms thereof, which are synthetically accessible via the subject methods and pharmaceutical compositions including the same.

公开(公告)号：US20190292172A1

公开(公告)日：2019-09-26

申请号：US16337221

申请日：2017-09-28

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Paul Wender ,  Ryan Quiroz ,  Stephen Ho ,  Akira Shimizu ,  Steven Ryckbosch ,  Matthew C. Stevens ,  Matthew S. Jeffreys ,  Clayton Hardman ,  Jack Sloane

IPC: C07D407/14

Abstract: Methods for preparing a variety of bryostatin compounds are provided. The subject methods provide for preparation of bryostatin 1 in multi-gram quantities in a low and unprecedented number of convergent synthetic steps from commercially available materials. The subject methods are scalable with low estimated material costs and can provide enough material to meet clinical needs. Also provided are a variety of bryostatin analog compounds, and prodrug forms thereof, which are synthetically accessible via the subject methods and pharmaceutical compositions including the same.

公开(公告)号：US20180028688A1

公开(公告)日：2018-02-01

申请号：US15662190

申请日：2017-07-27

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Robert M. Waymouth ,  Paul Wender ,  Jessica R. Vargas ,  Tim R. Blake ,  Colin McKinlay ,  Ronald Levy ,  Ole Audun Werner Haabeth ,  Nancy Benner ,  Katherine Near

IPC: A61K48/00 ,  C12N15/85 ,  A61K47/69 ,  C12N15/115

CPC classification number: A61K48/0041 ,  A61K47/593 ,  A61K47/6883 ,  A61K47/6907 ,  A61K47/6931 ,  C12N15/1135 ,  C12N15/115 ,  C12N15/85 ,  C12N15/88

Abstract: There are provided herein, inter alia, complexes, compositions and methods for the delivery of therapeutic, diagnostic and imaging agents, including nucleic acid, into a cell. The complexes, compositions and methods may facilitate complexation, protection, delivery and release of oligonucleotides and polyanionic cargos into target cells, tissues, and organs both in vitro and in vivo.

公开(公告)号：US09816066B2

公开(公告)日：2017-11-14

申请号：US14396274

申请日：2013-03-12

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Erika Geihe ,  Brian Trantow ,  Paul Wender ,  Joel M. Hyman ,  Bahram Parvin

IPC: C12N1/12 ,  A61K36/02 ,  A61K36/05 ,  C12Q1/24 ,  C12N15/87

CPC classification number: C12N1/12 ,  A61K36/02 ,  A61K36/05 ,  C07K2319/10 ,  C12N15/87 ,  C12Q1/24 ,  G01N2333/405

Abstract: The introduction of tools to study, control or expand the inner-workings of algae has been slow to develop. Provided are embodiments of a molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing molecular cargos into algal cells. The methods of the disclosure have been shown to work in wild-type algae that have an intact cell wall. Developed using Chlamydomonas reinhardtii, this method is also successful with less studied algae, including Neochloris oleoabundans and Scenedesmus dimorphus, thus providing a new and versatile tool for algal research and modification. The method of delivering a cargo compound to an algal cell comprises contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell, whereby the guanidinium-rich molecular transporter can traverse the algal cell wall, thereby delivering the cargo compound to the algal cell.

公开(公告)号：US12194105B2

公开(公告)日：2025-01-14

申请号：US16968834

申请日：2019-02-21

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Melanie Huttner ,  Paul Wender ,  Lynette Cegelski ,  Xiaoyu Zang ,  Alexandra Antonoplis

IPC: A61K47/64 ,  A61K38/14 ,  A61K47/54 ,  A61P31/04

Abstract: Antibiotic agents conjugated to a guanidinium-rich molecular transporter (GR-MoTr) are provided. The drug conjugates show surprising increases in efficacy compared to the unconjugated drug in difficult-to-treat bacterial infections including biofilms, stationary and persister cells, and multi-drug resistant bacteria, as well as intracellular bacteria.

公开(公告)号：US20240277870A1

公开(公告)日：2024-08-22

申请号：US18602410

申请日：2024-03-12

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Robert M Waymouth ,  Paul Wender ,  Jessica R. Vargas ,  Tim R. Blake ,  Colin J. McKinlay ,  Ronald Levy ,  Ole Audun Werner Haabeth ,  Nancy Benner ,  Katherine Near

IPC: A61K48/00 ,  A61K47/59 ,  A61K47/68 ,  A61K47/69 ,  C12N15/113 ,  C12N15/115 ,  C12N15/85 ,  C12N15/88

CPC classification number: A61K48/0041 ,  A61K47/593 ,  A61K47/6907 ,  A61K47/6931 ,  C12N15/115 ,  C12N15/85 ,  C12N15/88 ,  A61K47/6883 ,  C12N15/1135

Abstract: There are provided herein, inter alia, complexes, compositions and methods for the delivery of therapeutic, diagnostic and imaging agents, including nucleic acid, into a cell. The complexes, compositions and methods may facilitate complexation, protection, delivery and release of oligonucleotides and polyanionic cargos into target cells, tissues, and organs both in vitro and in vivo.

公开(公告)号：US11931416B2

公开(公告)日：2024-03-19

申请号：US17427362

申请日：2020-01-31

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

Inventor： Tim R. Blake ,  Paul Wender ,  Robert M. Waymouth ,  Ronald Levy ,  Ole Audun Werner Haabeth ,  Rebecca McClellan ,  Adrienne Sallets

IPC: A61K48/00 ,  A61K9/00 ,  A61K47/54 ,  A61K47/59 ,  C08G63/08 ,  C08G63/64 ,  C08G63/685 ,  C08G73/02

CPC classification number: A61K47/593 ,  A61K9/007 ,  A61K47/549 ,  A61K48/0041 ,  C08G63/08 ,  C08G63/64 ,  C08G63/685 ,  C08G73/028 ,  C08G73/0293

Abstract: There are provided herein, inter alia, cationic amphipathic polymers, complexes, and compositions comprising same, and methods for their use including for the delivery of therapeutic, diagnostic and imaging agents, including nucleic acids, into a cell. The complexes, compositions and methods may facilitate delivery and targeted release of the therapeutic, diagnostic and imaging agents to particular cell types and tissues.

公开(公告)号：US11746105B2

公开(公告)日：2023-09-05

申请号：US17162554

申请日：2021-01-29

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Paul Wender ,  Ryan Quiroz ,  Stephen Ho ,  Akira Shimizu ,  Steven Ryckbosch ,  Matthew C. Stevens ,  Matthew S. Jeffreys ,  Clayton Hardman ,  Jack Sloane

IPC: C07D407/14 ,  A61K31/351 ,  A61P25/28 ,  A61P31/18 ,  C07D493/22 ,  A61P35/00 ,  A61K31/365

CPC classification number: C07D407/14 ,  A61K31/351 ,  A61K31/365 ,  A61P25/28 ,  A61P31/18 ,  A61P35/00 ,  C07D493/22

Abstract: Methods for preparing a variety of bryostatin compounds are provided. The subject methods provide for preparation of bryostatin 1 in multi-gram quantities in a low and unprecedented number of convergent synthetic steps from commercially available materials. The subject methods are scalable with low estimated material costs and can provide enough material to meet clinical needs. Also provided are a variety of bryostatin analog compounds, and prodrug forms thereof, which are synthetically accessible via the subject methods and pharmaceutical compositions including the same.

公开(公告)号：US20150118704A1

公开(公告)日：2015-04-30

申请号：US14396274

申请日：2013-03-12

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Erika Geihe ,  Brian Trantow ,  Paul Wender ,  Joel M. Hyman ,  Bahram Parvin

IPC: C12N1/12 ,  C12Q1/24

CPC classification number: C12N1/12 ,  A61K36/02 ,  A61K36/05 ,  C07K2319/10 ,  C12N15/87 ,  C12Q1/24 ,  G01N2333/405

Abstract: The introduction of tools to study, control or expand the inner-workings of algae has been slow to develop. Provided are embodiments of a molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing molecular cargos into algal cells. The methods of the disclosure have been shown to work in wild-type algae that have an intact cell wall. Developed using Chlamydomonas reinhardtii, this method is also successful with less studied algae, including Neochloris oleoabundans and Scenedesmus dimorphus, thus providing a new and versatile tool for algal research and modification. The method of delivering a cargo compound to an algal cell comprises contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell, whereby the guanidinium-rich molecular transporter can traverse the algal cell wall, thereby delivering the cargo compound to the algal cell.

Abstract translation: 引入研究，控制或扩大藻类内部工作的工具进展缓慢。 提供了一种基于富含胍盐的分子转运体（GR-MoTrs）的分子方法的实施方案，用于将分子货物引入藻类细胞。 已经显示了本公开的方法在具有完整细胞壁的野生型藻类中起作用。 开发使用莱茵衣藻，这种方法也成功地研究了较少研究的藻类，包括新氯酸盐和双翅目，从而为藻类研究和修饰提供了一个新的和多功能的工具。 将货物化合物递送到藻类细胞的方法包括使藻类细胞与富含胍盐的递送载体接触，所述载体包含富含胍盐的分子转运蛋白（GR-MoTr），其与期待输送至藻类细胞的货物化合物连接，由此 富卟啉分子转运蛋白可以穿过藻细胞壁，从而将货物化合物递送到藻类细胞。