公开(公告)号：US11828755B2

公开(公告)日：2023-11-28

申请号：US16307125

申请日：2017-06-07

申请人： The Regents of the University of California

发明人： Daniel Takashi Kamei ,  Benjamin Ming Wu ,  Daniel William Bradbury ,  Shin Ting Sherine Frieda Cheung

IPC分类号： G01N33/543 ,  C12Q1/6844 ,  C12Q1/6813

CPC分类号： G01N33/54388 ,  C12Q1/6813 ,  C12Q1/6844 ,  G01N33/543 ,  G01N33/54306 ,  C12Q1/6813 ,  C12Q2563/159 ,  C12Q2565/625 ,  C12Q1/6844 ,  C12Q2521/513 ,  C12Q2531/119 ,  C12Q2531/143 ,  C12Q2563/159

摘要： In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a “one-pot” system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.

公开(公告)号：US20190250156A1

公开(公告)日：2019-08-15

申请号：US16307125

申请日：2017-06-07

申请人： The Regents of the University of California

发明人： Daniel Takashi Kamei ,  Benjamin Ming Wu ,  Daniel William Bradbury ,  Shin Ting Sherine Frieda Cheung

IPC分类号： G01N33/543

CPC分类号： G01N33/54386 ,  C12Q1/6813 ,  C12Q1/6844 ,  G01N33/53 ,  G01N33/543 ,  G01N33/54306 ,  G01N33/558 ,  C12Q2563/159 ,  C12Q2565/625 ,  C12Q2521/513 ,  C12Q2531/119 ,  C12Q2531/143

摘要： In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a “one-pot” system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.