公开(公告)号：US20240352501A1

公开(公告)日：2024-10-24

申请号：US18757644

申请日：2024-06-28

申请人： The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization

发明人： Maxim ITKIN ,  Rachel DAVIDOVICH-RIKANATI ,  Shahar COHEN ,  Vitaly PORTNOY ,  Adi DORON-FAIGENBOIM ,  Marina PETREIKOV ,  Shmuel SHEN ,  Yaakov TADMOR ,  Yosef BURGER ,  Efraim LEWINSOHN ,  Nurit KATZIR ,  Arthur Aaron SCHAFFER ,  Elad OREN

IPC分类号： C12P33/12 ,  A23L2/60 ,  A23L27/30 ,  C07J17/00 ,  C12N9/02 ,  C12N9/14 ,  C12N9/88 ,  C12N9/90 ,  C12P19/56 ,  C12P33/20

CPC分类号： C12P33/12 ,  A23L2/60 ,  A23L27/36 ,  C07J17/005 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/14 ,  C12N9/88 ,  C12N9/90 ,  C12P19/56 ,  C12P33/20 ,  C12Y114/99007 ,  C12Y204/02017 ,  C12Y402/01 ,  C12Y504/99033 ,  A23V2002/00

摘要： Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.

公开(公告)号：US20220170063A1

公开(公告)日：2022-06-02

申请号：US17563127

申请日：2021-12-28

申请人： The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization

发明人： Maxim ITKIN ,  Rachel DAVIDOVICH-RIKANATI ,  Shahar COHEN ,  Vitaly PORTNOY ,  Adi DORON-FAIGENBOIM ,  Marina PETREIKOV ,  Shmuel SHEN ,  Yaakov TADMOR ,  Yosef BURGER ,  Efraim LEWINSOHN ,  Nurit KATZIR ,  Arthur A. SCHAFFER ,  Elad OREN

IPC分类号： C12P33/12 ,  A23L27/30 ,  C12N9/90 ,  A23L2/60 ,  C12N9/14 ,  C12P33/20 ,  C12P19/56 ,  C07J17/00 ,  C12N9/02 ,  C12N9/88

摘要： Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.