公开(公告)号：US20230264191A1

公开(公告)日：2023-08-24

申请号：US18112279

申请日：2023-02-21

Applicant: The Texas A&M University System

Inventor： Abhishek Jain ,  Amirali Selahi

IPC: B01L3/00 ,  C12N5/071

CPC classification number: B01L3/502707 ,  B01L3/502761 ,  C12N5/0602 ,  B01L3/502715 ,  B01L2300/0816 ,  B01L2400/0487 ,  B01L2300/0864 ,  B01L2200/025 ,  B01L2200/10

Abstract: A method for fabricating an in vitro vessel includes forming a substrate that defines a microfluidic passage therein extending along a longitudinal axis and defined by an inner surface, positioning the substrate in a vertical orientation whereby an acute angle is formed between the longitudinal axis of the microfluidic passage and the direction of gravity, and culturing a plurality of first cells in the microfluidic passage while the substrate is disposed in the vertical orientation whereby an annular layer of the plurality of first cells is formed in the microfluidic channel, wherein the layer of the plurality of first cells defines a lumen extending longitudinally through the microfluidic channel.