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    Compositions and methods for self-regulated inducible gene expression
    6.
    发明授权
    Compositions and methods for self-regulated inducible gene expression 有权

    公开(公告)号：US10392622B2

    公开(公告)日：2019-08-27

    申请号：US15500591

    申请日：2015-07-31

    申请人： The Trustees of the University of Pennsylvania

    发明人： Mitchell Lewis ,  Jean Bennett ,  Luk Vandenberghe ,  Matthew Sochor ,  Theodore G. Drivas

    IPC分类号： C12N15/63

    摘要： A self-regulating gene expression construct comprises a single promoter in operative association with a repressor sequence (e.g., bacterial repressor lacI or gaiR), operator sequence(s) responsive to the expressed repressor protein, and a transgene. A dual-regulating construct comprises a single promoter controlling expression of a bacterial repressor sequence and a transgene, and which, in the presence of a first inducer molecule, transcribes the transgene and repressor; and a ribozyme in association with an aptamer sequence, the aptamer sequence capable of interacting with a second inducer molecule to terminate mRNA degradation by the ribozyme. Also provided are recombinant vectors or viruses containing the self-regulating or dual self-regulating constructs and cells containing the vectors. Such compositions are useful in methods of treating a diseases using gene therapy.