公开(公告)号：US10980872B2

公开(公告)日：2021-04-20

申请号：US16425725

申请日：2019-05-29

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter L. Collins ,  Cindy L. Luongo ,  Ursula J. Buchholz ,  Brian R. Murphy

IPC分类号： A61K39/155 ,  A61K39/12 ,  C07K14/005 ,  C12N7/00 ,  A61K39/00

摘要： Provided herein are recombinant respiratory syncytial viruses that contain mutations that make the disclosed viruses attractive vaccine candidates. The viruses disclosed contain attenuating mutations designed to have increased genetic and phenotypic stability. Desired combinations of these mutations can be made to achieve desired levels of attenuation. Exemplary vaccine candidates are described. Also provided are polynucleotides capable of encoding the described viruses, as wells as methods for producing the viruses and methods of use.

公开(公告)号：US10058602B2

公开(公告)日：2018-08-28

申请号：US14305572

申请日：2014-06-16

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services ,  The United States of America, as Represented by The Secretary of The Army

发明人： Alexander G. Pletnev ,  Joseph R. Putnak ,  Robert M. Chanock ,  Brian R. Murphy ,  Stephen S. Whitehead ,  Joseph E. Blaney

IPC分类号： A61K45/06 ,  C12N7/00 ,  A61K39/12 ,  C07K14/005 ,  C12N15/86 ,  C12Q1/6888 ,  A61K39/00

CPC分类号： A61K39/12 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/24122 ,  C12N2770/24134 ,  C12N2770/24143 ,  C12N2770/24162 ,  C12Q1/6888 ,  Y02A50/386 ,  Y02A50/394

摘要： The present invention relates to attenuated, immunogenic West Nile virus chimeras built on a dengue virus backbone for the production of immunogenic, live, attenuated West Nile virus vaccines.

公开(公告)号：US09884104B2

公开(公告)日：2018-02-06

申请号：US15018857

申请日：2016-02-08

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Stephen S. Whitehead ,  Richard S. Bennett ,  Brian R. Murphy

IPC分类号： A61K39/12 ,  C12Q1/70 ,  C12N7/00 ,  C07K14/005 ,  A61K39/00

CPC分类号： A61K39/12 ,  A61K39/00 ,  A61K2039/5252 ,  A61K2039/5254 ,  C07K14/005 ,  C12N7/00 ,  C12N2760/12022 ,  C12N2760/12034 ,  C12Q1/701

摘要： The invention relates to vaccine compositions including CEV serogroup immunogens, attenuated and inactivated viruses of the CEV serogroup and chimeric Bunyaviridae. Also disclosed are methods of treating or preventing CEV serogroup infection in a mammalian host, methods of producing a subunit vaccine composition or an immunogenic composition, isolated polynucleotides comprising a nucleotide sequence encoding a CEV serogroup immunogen, methods for detecting La Crosse virus (LACY) infection in a biological sample and infectious chimeric Bunyaviridae.

公开(公告)号：US09624475B2

公开(公告)日：2017-04-18

申请号：US14394226

申请日：2013-03-13

申请人： THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： Peter L. Collins ,  Cindy L. Luongo ,  Ursula J. Buchholz ,  Brian R. Murphy

IPC分类号： C12N7/00 ,  A61K39/12 ,  C07K14/005 ,  A61K39/00

CPC分类号： A61K39/155 ,  A61K39/12 ,  A61K2039/5254 ,  C07K14/005 ,  C12N7/00 ,  C12N2760/18521 ,  C12N2760/18522 ,  C12N2760/18534 ,  C12N2760/18562

摘要： Provided herein are recombinant respiratory syncytial viruses that contain mutations that make the disclosed viruses attractive vaccine candidates. The viruses disclosed contain attenuating mutations designed to have increased genetic and phenotypic stability. Desired combinations of these mutations can be made to achieve desired levels of attenation. Exemplary vaccine candidates are described. Also provided are polynucleotides capable of encoding the described viruses, as wells as methods for producing the viruses and methods of use.

公开(公告)号：US10307476B2

公开(公告)日：2019-06-04

申请号：US15455438

申请日：2017-03-10

申请人： THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： Peter L. Collins ,  Cindy L. Luongo ,  Ursula J. Buchholz ,  Brian R. Murphy

IPC分类号： A61K39/155 ,  A61K39/12 ,  C07K14/005 ,  C12N7/00 ,  A61K39/00

摘要： Provided herein are recombinant respiratory syncytial viruses that contain mutations that make the disclosed viruses attractive vaccine candidates. The viruses disclosed contain attenuating mutations designed to have increased genetic and phenotypic stability. Desired combinations of these mutations can be made to achieve desired levels of attenation. Exemplary vaccine candidates are described. Also provided are polynucleotides capable of encoding the described viruses, as wells as methods for producing the viruses and methods of use.

公开(公告)号：US10160957B2

公开(公告)日：2018-12-25

申请号：US14742533

申请日：2015-06-17

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Stephen S. Whitehead ,  Joseph E. Blaney ,  Brian R. Murphy ,  Ching-Juh Lai

IPC分类号： C12N7/04 ,  C12N7/00 ,  C07K14/18 ,  C07K14/005 ,  A61K39/00

摘要： The invention is related to a dengue virus or chimeric dengue virus that contains a mutation in the 3′ untranslated region (3′-UTR) comprising a Δ30 mutation that removes the TL-2 homologous structure in each of the dengue virus serotypes 1, 2, 3, and 4, and nucleotides additional to the Δ30 mutation deleted from the 3′-UTR that removes sequence in the 5′ direction as far as the 5′ boundary of the TL-3 homologous structure in each of the dengue virus serotypes 1, 2, 3, and 4, or a replacement of the 3′-UTR of a dengue virus of a first serotype with the 3′-UTR of a dengue virus of a second serotype, optionally containing the Δ30 mutation and nucleotides additional to the Δ30 mutation deleted from the 3′-UTR; and immunogenic compositions, methods of inducing an immune response, and methods of producing a dengue virus or chimeric dengue virus.