公开(公告)号：US08802835B2

公开(公告)日：2014-08-12

申请号：US13869660

申请日：2013-04-24

申请人： The United States of Americas as represented by the Department of Veterans Affairs ,  The Regents of the University of California ,  Cornell Research Foundation, Inc. ,  Fundação Oswaldo Cruz—FIOCRUZ

发明人： Albert I. Ko ,  Mitermayer Galvão Reis ,  Julio Henrique Rosa Croda ,  Isadora Cristina Siqueira ,  David A. Haake ,  James Matsunaga ,  Lee W. Riley ,  Michele Barocchi ,  Tracy Ann Young

IPC分类号： C07H21/02

CPC分类号： C07K16/1207 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/20 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2469/20 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

摘要翻译： 本发明涉及编码具有重复的细菌Ig样（Big）结构域的钩端螺菌属细菌中的蛋白质BigL1，BigL2和BigL3的三种分离的DNA分子，以及它们在诊断，治疗和疫苗应用中的应用。 根据本发明，编码BigL1，BigL2和BigL3蛋白的分离的分子用于诊断和预防能够在人和其他哺乳动物（包括具有兽医重要性的哺乳动物）中产生疾病的钩端螺旋体属物种的感染。

公开(公告)号：US20170037114A1

公开(公告)日：2017-02-09

申请号：US15334128

申请日：2016-10-25

申请人： U.S. Government represented by the Department of Veterans Affairs ,  The Regents of the University of California ,  Cornell Research Foundation, Inc. ,  Fundação Oswaldo Cruz - FIOCRUZ

发明人： Albert I. Ko ,  Mitermayer Galvão Reis ,  Julio Henrique Rosa Croda ,  Isadora Cristina Siqueira ,  David A. Haake ,  James Matsunaga ,  Lee W. Riley ,  Michele Barocchi ,  Tracy Ann Young

IPC分类号： C07K16/12 ,  G01N33/569 ,  C12Q1/68

CPC分类号： C07K16/1207 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/20 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2469/20 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

摘要翻译： 本发明涉及编码具有重复的细菌Ig样（Big）结构域的钩端螺菌属细菌中的蛋白质BigL1，BigL2和BigL3的三种分离的DNA分子，以及它们在诊断，治疗和疫苗应用中的应用。 根据本发明，编码BigL1，BigL2和BigL3蛋白的分离的分子用于诊断和预防能够在人和其他哺乳动物（包括具有兽医重要性的哺乳动物）中产生疾病的钩端螺旋体属物种的感染。

公开(公告)号：US09133250B2

公开(公告)日：2015-09-15

申请号：US14281580

申请日：2014-05-19

申请人： The United States of America represented by the Department of Veterans Affairs ,  The Regents of the University of California ,  Cornell Research Foundation, Inc. ,  Fundação Oswaldo Cruz—FIOCRUZ

发明人： Albert I. Ko ,  Mitermayer Galvão Reis ,  Julio Henrique Rosa Croda ,  Isadora Cristina Siqueira ,  David A. Haake ,  James Matsunaga ,  Lee W. Riley ,  Michele Barocchi ,  Tracy Ann Young

IPC分类号： C12N15/00 ,  C07K14/20 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C07K16/1207 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/20 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2469/20 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

摘要翻译： 本发明涉及编码具有重复的细菌Ig样（Big）结构域的钩端螺菌属细菌中的蛋白质BigL1，BigL2和BigL3的三种分离的DNA分子，以及它们在诊断，治疗和疫苗应用中的应用。 根据本发明，编码BigL1，BigL2和BigL3蛋白的分离的分子用于诊断和预防能够在人和其他哺乳动物（包括具有兽医重要性的哺乳动物）中产生疾病的钩端螺旋体属物种的感染。

公开(公告)号：US20140308734A1

公开(公告)日：2014-10-16

申请号：US14281580

申请日：2014-05-19

申请人： The United States Government represented by the Department of Veterans Affairs ,  The Regents of the University of California ,  Cornell Research Foundation, Inc. ,  Fundação Oswaldo Cruz - FIOCRUZ

发明人： Albert I. Ko ,  Mitermayer Galvão Reis ,  Julio Henrique Rosa Croda ,  Isadora Cristina Siqueira ,  David A. Haake ,  James Matsunaga ,  Lee W. Riley ,  Michele Barocchi ,  Tracy Ann Young

IPC分类号： C07K14/20

CPC分类号： C07K16/1207 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/20 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2469/20 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

摘要翻译： 本发明涉及编码具有重复的细菌Ig样（Big）结构域的钩端螺菌属细菌中的蛋白质BigL1，BigL2和BigL3的三种分离的DNA分子，以及它们在诊断，治疗和疫苗应用中的应用。 根据本发明，编码BigL1，BigL2和BigL3蛋白的分离的分子用于诊断和预防能够在人和其他哺乳动物（包括具有兽医重要性的哺乳动物）中产生疾病的钩端螺旋体属物种的感染。

公开(公告)号：US20180186867A1

公开(公告)日：2018-07-05

申请号：US15899805

申请日：2018-02-20

申请人： U.S. Government represented by the Department of Veterans Affairs ,  The Regents of the University of California ,  Cornell Research Foundation, Inc. ,  Fundação Oswaldo Cruz - FIOCRUZ

发明人： Albert I. Ko ,  Mitermayer Galvão Reis ,  Julio Henrique Rosa Croda ,  Isadora Cristina Siqueira ,  David A. Haake ,  James Matsunaga ,  Lee W. Riley ,  Michele Barocchi ,  Tracy Ann Young

IPC分类号： C07K16/12 ,  C12Q1/689 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C07K16/1207 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/20 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2469/20 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

公开(公告)号：US20130230903A1

公开(公告)日：2013-09-05

申请号：US13869660

申请日：2013-04-24

申请人： The United States Government represented by the Department of Veterans Affairs ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  CORNELL RESEARCH FOUNDATION, INC. ,  FUNDAÇÃO OSWALDO CRUZ - FIOCRUZ

发明人： Albert I. Ko ,  Mitermayer Galvão Reis ,  Julio Henrique Rosa Croda ,  Isadora Cristina Siqueira ,  David A. Haake ,  James Matsunaga ,  Lee W. Riley ,  Michele Barocchi ,  Tracy Ann Young

IPC分类号： C07K14/20

CPC分类号： C07K16/1207 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/20 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2469/20 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

公开(公告)号：US09932391B2

公开(公告)日：2018-04-03

申请号：US15334128

申请日：2016-10-25

申请人： The United States of America represented by the Department of Veterans Affairs ,  The Regents of the University of California ,  Cornell Research Foundation, Inc. ,  Fundação Oswaldo Cruz—FIOCRUZ

发明人： Albert I. Ko ,  Mitermayer Galvão Reis ,  Julio Henrique Rosa Croda ,  Isadora Cristina Siqueira ,  David A. Haake ,  James Matsunaga ,  Lee W. Riley ,  Michele Barocchi ,  Tracy Ann Young

IPC分类号： G01N33/53 ,  C07K16/12 ,  C12Q1/68 ,  C07H21/04 ,  C07K14/20 ,  G01N33/569 ,  A61K39/00

CPC分类号： C07K16/1207 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/20 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2469/20 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

公开(公告)号：US20160222069A1

公开(公告)日：2016-08-04

申请号：US15134168

申请日：2016-04-20

申请人： U.S. Government represented by the Department of Veterans Affairs ,  The Regents of the University of California ,  Cornell Research Foundation, Inc. ,  Fundação Oswaldo Cruz - FIOCRUZ

发明人： Albert I. Ko ,  Mitermayer Galvão Reis ,  Julio Henrique Rosa Croda ,  Isadora Cristina Siqueira ,  David A. Haake ,  James Matsunaga ,  Lee W. Riley ,  Michele Barocchi ,  Tracy Ann Young

IPC分类号： C07K14/20

CPC分类号： C07K16/1207 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/20 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2469/20 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

公开(公告)号：US09346858B2

公开(公告)日：2016-05-24

申请号：US14819045

申请日：2015-08-05

申请人： The United States of America as represented by the Department of Veterans Affairs ,  The Regents of the University of California ,  Cornell Research Foundation, Inc. ,  Fundação Oswaldo Cruz—FIOCRUZ

发明人： Albert I. Ko ,  Mitermayer Galvão Reis ,  Julio Henrique Rosa Croda ,  Isadora Cristina Siqueira ,  David A. Haake ,  James Matsunaga ,  Lee W. Riley ,  Michele Barocchi ,  Tracy Ann Young

IPC分类号： C07H21/04 ,  C07K14/20 ,  C12Q1/68

CPC分类号： C07K16/1207 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/20 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2469/20 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

摘要翻译： 本发明涉及编码具有重复的细菌Ig样（Big）结构域的钩端螺菌属细菌中的蛋白质BigL1，BigL2和BigL3的三种分离的DNA分子，以及它们在诊断，治疗和疫苗应用中的应用。 根据本发明，编码BigL1，BigL2和BigL3蛋白的分离的分子用于诊断和预防能够在人和其他哺乳动物（包括具有兽医重要性的哺乳动物）中产生疾病的钩端螺旋体属物种的感染。

公开(公告)号：US20150329599A1

公开(公告)日：2015-11-19

申请号：US14819045

申请日：2015-08-05

申请人： The United States Government represented by the Department of Veterans Affairs ,  The Regents of the University of California ,  Cornell Research Foundation, Inc. ,  Fundação Oswaldo Cruz - FIOCRUZ

发明人： Albert I. Ko ,  Mitermayer Galvao Reis ,  Julio Henrique Rosa Croda ,  Isadora Cristina Siqueira ,  David A. Haake ,  James Matsunaga ,  Lee W. Riley ,  Michele Barocchi ,  Tracy Ann Young

IPC分类号： C07K14/20

CPC分类号： C07K16/1207 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/20 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2469/20 ,  Y02A50/56

摘要： The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in the Leptospira sp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection with Leptospira species that are capable of producing disease in humans and other mammals, including those of veterinary importance.

摘要翻译： 本发明涉及编码具有重复的细菌Ig样（Big）结构域的钩端螺菌属细菌中的蛋白质BigL1，BigL2和BigL3的三种分离的DNA分子，以及它们在诊断，治疗和疫苗应用中的应用。 根据本发明，编码BigL1，BigL2和BigL3蛋白的分离的分子用于诊断和预防能够在人和其他哺乳动物（包括具有兽医重要性的哺乳动物）中产生疾病的钩端螺旋体属物种的感染。