公开(公告)号：US4849356A

公开(公告)日：1989-07-18

申请号：US217471

申请日：1988-07-11

申请人： Theodorus J. G. M. Van Dooren ,  Johannes A. M. Van Balken

发明人： Theodorus J. G. M. Van Dooren ,  Johannes A. M. Van Balken

IPC分类号： C12N9/10 ,  C12P19/18 ,  C12R1/66

CPC分类号： C12N9/1051 ,  C12P19/18

摘要： Process for preparing a fructosyl transferase enzyme preparation by cultivating an Aspergillus species and recovering from the culture a preparation having fructosyl transferase activity, wherein Aspergillus phoenicis is cultivated and the mycelium is recovered from the culture medium.

公开(公告)号：US5248608A

公开(公告)日：1993-09-28

申请号：US819129

申请日：1992-01-10

申请人： Theodorus J. G. M. Van Dooren ,  Wilhelmus J. J. Van Den Tweel

发明人： Theodorus J. G. M. Van Dooren ,  Wilhelmus J. J. Van Den Tweel

IPC分类号： C12P13/04 ,  C12P41/00 ,  C12R1/01 ,  C12R1/22

CPC分类号： C12P41/006 ,  C12P13/04 ,  Y10S435/822 ,  Y10S435/824

摘要： Enzyme-catalysed preparation of an .alpha.-substituted optically active carboxylic acid, using enantioselective hydrolysis of a mixture of the enantiomers of the corresponding amide, an Ochrobactrum anthropi or an Klebsiella sp. being used as the enzyme. During the hydrolysis both the L-carboxylic acid and the remaining D-carboxylic acid amide are obtained with high optical purity and high yield, while the activity of the micro-organism is high. A large number of .alpha.-substituted carboxylic acid amides with a remarkable structural variety can be hydrolysed in this manner.

公开(公告)号：US5527689A

公开(公告)日：1996-06-18

申请号：US366592

申请日：1994-12-29

申请人： Shigeaki Irino ,  Shin-ichiro Nakamura ,  Kiyotaka Oyama ,  Peter J. L. M. Quaedflieg ,  Theodorus J. G. M. Van Dooren

发明人： Shigeaki Irino ,  Shin-ichiro Nakamura ,  Kiyotaka Oyama ,  Peter J. L. M. Quaedflieg ,  Theodorus J. G. M. Van Dooren

IPC分类号： A23L27/30 ,  C07K5/072 ,  C12P21/02 ,  C12N9/50 ,  C12N9/52 ,  C12N9/54

CPC分类号： C07K5/0613

摘要： The invention relates to a process for the preparation of N-benzyloxycarbonyl-.alpha.-L-aspartyl-L-phenylalanine methyl ester by enzymatic coupling of N-benzyloxycarbonyl-L-aspartic acid and L-phenylalanine methyl ester in an aqueous medium with formation of a precipitate, the coupling reaction being effected with (virtually) equimolar quantities of N-benzyloxycarbonyl-L-aspartic acid and L-phenylalanine methyl ester under the influence of a neutral protease at an initial pH of from 4.5 to 6.0 and in the presence of from 3 to 25%, calculated as per cent by weight based on the total reaction mixture, of an alkali metal salt, alkaline earth metal salt or ammonium salt.

摘要翻译： 本发明涉及通过将N-苄氧羰基-L-天冬氨酸和L-苯基丙氨酸甲酯在水性介质中酶促偶联制备N-苄氧羰基-α-L-天冬氨酰基-L-苯丙氨酸甲酯的方法，形成 沉淀，偶联反应用（实际）等摩尔量的N-苄氧羰基-L-天冬氨酸和L-苯丙氨酸甲酯在中性蛋白酶的影响下在初始pH为4.5至6.0的条件下进行，并且在 3重量％至25重量％，以总反应混合物的重量计，碱金属盐，碱土金属盐或铵盐计算。