公开(公告)号：US20080227140A1

公开(公告)日：2008-09-18

申请号：US12040524

申请日：2008-02-29

申请人： Thijs Kaper ,  Michael Platten ,  Lawrence Steinman ,  Wolf Frommer

发明人： Thijs Kaper ,  Michael Platten ,  Lawrence Steinman ,  Wolf Frommer

IPC分类号： C12Q1/02 ,  C12N5/06

CPC分类号： G01N33/6872 ,  G01N33/5008 ,  G01N2333/70596 ,  G01N2800/24

摘要： The present invention provides methods for detecting changes in tryptophan concentrations in a cell and methods for identifying agents that modulate cellular tryptophan concentrations. In particular, the present invention provides methods for detecting cellular exchange between tryptophan and kynurenine, and methods for identifying agents that modulate this exchange. The present invention also provides methods for treating a disease associated with immunosuppression in a subject in need thereof. In particular, the present invention is directed toward a method of treating a disease associated with immunosuppression comprising contacting the disease with an agent that modulates cellular Trp/kynurenine exchange. Furthermore, the present invention provides methods for identifying an agent that modulates an immunosuppression.

摘要翻译： 本发明提供了用于检测细胞中色氨酸浓度变化的方法以及鉴定调节细胞色氨酸浓度的试剂的方法。 特别地，本发明提供了检测色氨酸和犬尿氨酸之间的细胞交换的方法，以及用于鉴定调节该交换的试剂的方法。 本发明还提供了在有需要的受试者中治疗与免疫抑制相关的疾病的方法。 特别地，本发明涉及一种治疗与免疫抑制相关的疾病的方法，其包括将疾病与调节细胞Trp /犬尿苷交换的药物接触。 此外，本发明提供了鉴定调节免疫抑制的药剂的方法。

公开(公告)号：US07935494B2

公开(公告)日：2011-05-03

申请号：US12040524

申请日：2008-02-29

申请人： Thijs Kaper ,  Michael Platten ,  Lawrence Steinman ,  Wolf Frommer

发明人： Thijs Kaper ,  Michael Platten ,  Lawrence Steinman ,  Wolf Frommer

IPC分类号： G01N33/53

CPC分类号： G01N33/6872 ,  G01N33/5008 ,  G01N2333/70596 ,  G01N2800/24

摘要： The present invention provides methods for detecting changes in tryptophan concentrations in a cell and methods for identifying agents that modulate cellular tryptophan concentrations. In particular, the present invention provides methods for detecting cellular exchange between tryptophan and kynurenine, and methods for identifying agents that modulate this exchange. The present invention also provides methods for treating a disease associated with immunosuppression in a subject in need thereof. In particular, the present invention is directed toward a method of treating a disease associated with immunosuppression comprising contacting the disease with an agent that modulates cellular Trp/kynurenine exchange. Furthermore, the present invention provides methods for identifying an agent that modulates an immunosuppression.

摘要翻译： 本发明提供了用于检测细胞中色氨酸浓度变化的方法以及鉴定调节细胞色氨酸浓度的试剂的方法。 特别地，本发明提供了检测色氨酸和犬尿氨酸之间的细胞交换的方法，以及用于鉴定调节该交换的试剂的方法。 本发明还提供了在有需要的受试者中治疗与免疫抑制相关的疾病的方法。 特别地，本发明涉及一种治疗与免疫抑制相关的疾病的方法，其包括将疾病与调节细胞Trp /犬尿苷交换的药物接触。 此外，本发明提供了鉴定调节免疫抑制的药剂的方法。

公开(公告)号：US08071744B2

公开(公告)日：2011-12-06

申请号：US13096643

申请日：2011-04-28

申请人： Thijs Kaper ,  Michael Platten ,  Lawrence Steinman ,  Wolf Frommer

发明人： Thijs Kaper ,  Michael Platten ,  Lawrence Steinman ,  Wolf Frommer

IPC分类号： C12N15/62

CPC分类号： G01N33/6872 ,  G01N33/5008 ,  G01N2333/70596 ,  G01N2800/24

摘要： The present invention provides methods for detecting changes in tryptophan concentrations in a cell and methods for identifying agents that modulate cellular tryptophan concentrations. In particular, the present invention provides methods for detecting cellular exchange between tryptophan and kynurenine, and methods for identifying agents that modulate this exchange. The present invention also provides methods for treating a disease associated with immunosuppression in a subject in need thereof. In particular, the present invention is directed toward a method of treating a disease associated with immunosuppression comprising contacting the disease with an agent that modulates cellular Trp/kynurenine exchange. Furthermore, the present invention provides methods for identifying an agent that modulates an immunosuppression.

摘要翻译： 本发明提供了用于检测细胞中色氨酸浓度变化的方法以及鉴定调节细胞色氨酸浓度的试剂的方法。 特别地，本发明提供了检测色氨酸和犬尿氨酸之间的细胞交换的方法，以及用于鉴定调节该交换的试剂的方法。 本发明还提供了在有需要的受试者中治疗与免疫抑制相关的疾病的方法。 特别地，本发明涉及一种治疗与免疫抑制相关的疾病的方法，其包括将疾病与调节细胞Trp /犬尿苷交换的药物接触。 此外，本发明提供了鉴定调节免疫抑制的药剂的方法。

公开(公告)号：US20110223657A1

公开(公告)日：2011-09-15

申请号：US13096643

申请日：2011-04-28

申请人： Thijs Kaper ,  Michael Platten ,  Lawrence Steinman ,  Wolf Frommer

发明人： Thijs Kaper ,  Michael Platten ,  Lawrence Steinman ,  Wolf Frommer

IPC分类号： C07H21/00 ,  C12N15/63 ,  C12N5/10 ,  C12N1/00

CPC分类号： G01N33/6872 ,  G01N33/5008 ,  G01N2333/70596 ,  G01N2800/24

摘要： The present invention provides methods for detecting changes in tryptophan concentrations in a cell and methods for identifying agents that modulate cellular tryptophan concentrations. In particular, the present invention provides methods for detecting cellular exchange between tryptophan and kynurenine, and methods for identifying agents that modulate this exchange. The present invention also provides methods for treating a disease associated with immunosuppression in a subject in need thereof. In particular, the present invention is directed toward a method of treating a disease associated with immunosuppression comprising contacting the disease with an agent that modulates cellular Trp/kynurenine exchange. Furthermore, the present invention provides methods for identifying an agent that modulates an immunosuppression.

摘要翻译： 本发明提供了用于检测细胞中色氨酸浓度变化的方法以及鉴定调节细胞色氨酸浓度的试剂的方法。 特别地，本发明提供了检测色氨酸和犬尿氨酸之间的细胞交换的方法，以及用于鉴定调节该交换的试剂的方法。 本发明还提供了在有需要的受试者中治疗与免疫抑制相关的疾病的方法。 特别地，本发明涉及一种治疗与免疫抑制相关的疾病的方法，其包括将疾病与调节细胞Trp /犬尿苷交换的药物接触。 此外，本发明提供了鉴定调节免疫抑制的药剂的方法。

公开(公告)号：US20080019920A1

公开(公告)日：2008-01-24

申请号：US11665343

申请日：2005-10-14

申请人： Sakiko Okumoto ,  Loren Looger ,  Wolf Frommer

发明人： Sakiko Okumoto ,  Loren Looger ,  Wolf Frommer

IPC分类号： C12Q1/68 ,  A01K67/00 ,  A61K49/00 ,  C07H21/00 ,  C12N1/00 ,  C12N1/16 ,  C12N1/20 ,  C12N15/63 ,  C12N5/06

CPC分类号： G01N33/542 ,  G01N33/9406 ,  G01N2500/00

摘要： Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.

摘要翻译： 公开了神经递质生物传感器，包括基于YbeJ的谷氨酸结合生物传感器，其包含与供体和荧光部分缀合的神经递质结合结构域，其允许在结合神经递质时检测和测量荧光共振能量转移。 这种生物传感器可用于体内和培养物中神经递质浓度的检测。