Detection and amplification of target nucleic acid sequences
    1.
    发明授权
    Detection and amplification of target nucleic acid sequences 失效
    靶核酸序列的检测和扩增

    公开(公告)号:US5858652A

    公开(公告)日:1999-01-12

    申请号:US331298

    申请日:1994-10-28

    IPC分类号: C12Q1/68 C12P19/34

    CPC分类号: C12Q1/686

    摘要: A method and kits for amplifying and detecting target nucleic acid sequences in a sample is disclosed. The method employs primers which have reactive pair members linked to them. The reactive pair members can be attached to a solid phase and/or detected by labeled conjugate.

    摘要翻译: 公开了用于扩增和检测样品中靶核酸序列的方法和试剂盒。 该方法使用具有与其连接的反应对成员的引物。 活性对成员可以连接到固相和/或通过标记的缀合物检测。

    Nucleotide sequences and process for amplifying and detection of
hepatitis B viral DNA
    2.
    发明授权
    Nucleotide sequences and process for amplifying and detection of hepatitis B viral DNA 失效
    用于扩增和检测乙型肝炎病毒DNA的核苷酸序列和方法

    公开(公告)号:US5736334A

    公开(公告)日:1998-04-07

    申请号:US758626

    申请日:1996-11-27

    申请人: Uwe Spies

    发明人: Uwe Spies

    IPC分类号: C12Q1/70 C12Q1/68 C07H21/04

    CPC分类号: C12Q1/706

    摘要: Short nucleotide sequences of hepatitis B virus useful for the determination of the presence and type of hepatitis B virus present in a test sample. The sequences provided can be amplified by various DNA hybridization techniques including a modified polymerase chain reaction or ligase chain reaction. The sequences provided also can be hybridized by standard dot- or replica-blot procedures. Methods and kits also are provided for the detection of hepatitis B virus in a test sample and the determination of the type of hepatitis B virus present in the test sample.

    摘要翻译: 乙型肝炎病毒的短核苷酸序列可用于确定测试样品中存在的乙型肝炎病毒的存在和类型。 所提供的序列可以通过包括经修饰的聚合酶链反应或连接酶链反应的各种DNA杂交技术进行扩增。 提供的序列也可以通过标准点或复制印迹方法杂交。 还提供了用于在测试样品中检测乙型肝炎病毒的方法和试剂盒,以及确定测试样品中存在的乙型肝炎病毒的类型。