公开(公告)号：US20050079494A1

公开(公告)日：2005-04-14

申请号：US10409993

申请日：2003-04-09

申请人： Tim Oulmassov ,  William Rapp ,  Kairong Tian ,  Jason Wicker ,  William Welsh ,  Xia Wang ,  Robert DeLisle ,  Tyamagondlu Venkatesh ,  Jihong Liang

发明人： Tim Oulmassov ,  William Rapp ,  Kairong Tian ,  Jason Wicker ,  William Welsh ,  Xia Wang ,  Robert DeLisle ,  Tyamagondlu Venkatesh ,  Jihong Liang

IPC分类号： A01H1/00 ,  C12N5/04 ,  C12N15/82 ,  C12Q1/68

CPC分类号： C12N15/8254 ,  C12N15/8253

摘要： The present invention generally relates to the field of plant genetics and protein biochemistry. More specifically, the present invention relates to modified proteins having an increased number of essential amino acids. The present invention provides proteins modified to have an increased number of essential amino acids, nucleic acid sequences encoding the enhanced proteins, and methods of designing, producing, and using the same. The present invention also includes compositions, transformed host cells, transgenic plants, and seeds containing the enhanced proteins, and methods for preparing and using the same.

摘要翻译： 本发明一般涉及植物遗传学和蛋白质生物化学领域。 更具体地说，本发明涉及具有增加必需氨基酸数量的修饰蛋白质。 本发明提供了修饰为具有增加数量的必需氨基酸的蛋白质，编码增强蛋白质的核酸序列，以及设计，生产和使用该蛋白质的方法。 本发明还包括组合物，转化的宿主细胞，转基因植物和含有增强蛋白质的种子，以及制备和使用该蛋白质的方法。

公开(公告)号：US06927321B2

公开(公告)日：2005-08-09

申请号：US10015637

申请日：2001-12-17

申请人： Qi Wang ,  Patrice Dubois ,  Jihong Liang ,  Tim Oulmassov

发明人： Qi Wang ,  Patrice Dubois ,  Jihong Liang ,  Tim Oulmassov

IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  C12N5/82 ,  A01H5/00 ,  C12N5/04 ,  C12N15/11

CPC分类号： C07K14/415 ,  C12N15/8216 ,  C12N15/8234

摘要： The present invention discloses Arcelin promoters capable of transcribing a heterologous nucleic acid sequence at high levels in plants. The promoters are particularly suited for use in soybean plants and plant cells. Methods of modifying, producing, and using the promoters are also disclosed. The invention further discloses compositions, transformed host cells, transgenic plants, and seeds containing the high-expression Arcelin promoters, and methods for preparing and using the same.

摘要翻译： 本发明公开了能够在植物中以高水平转录异源核酸序列的佐剂启动子。 启动子特别适用于大豆植物和植物细胞。 还公开了改性，制备和使用促进剂的方法。 本发明进一步公开了组合物，转化的宿主细胞，转基因植物和含有高表达Arcelin启动子的种子及其制备和使用方法。

公开(公告)号：US20100251433A1

公开(公告)日：2010-09-30

申请号：US12699442

申请日：2010-02-03

申请人： Qi Wang ,  Patrice Dubois ,  Jihong Liang ,  Tim Oulmassov

发明人： Qi Wang ,  Patrice Dubois ,  Jihong Liang ,  Tim Oulmassov

IPC分类号： A01H5/00 ,  C12N5/10

CPC分类号： C07K14/415 ,  C12N15/8216 ,  C12N15/8234

摘要： The present invention discloses Arcelin promoters capable of transcribing a heterologous nucleic acid sequence at high levels in plants. The promoters are particularly suited for use in soybean plants and plant cells. Methods of modifying, producing, and using the promoters are also disclosed. The invention further discloses compositions, transformed host cells, transgenic plants, and seeds containing the high-expression Arcelin promoters, and methods for preparing and using the same.

摘要翻译： 本发明公开了能够在植物中以高水平转录异源核酸序列的佐剂启动子。 启动子特别适用于大豆植物和植物细胞。 还公开了改性，制备和使用促进剂的方法。 本发明进一步公开了组合物，转化的宿主细胞，转基因植物和含有高表达Arcelin启动子的种子及其制备和使用方法。

公开(公告)号：US20050241018A1

公开(公告)日：2005-10-27

申请号：US11148661

申请日：2005-06-09

申请人： Qi Wang ,  Patrice Dubois ,  Jihong Liang ,  Tim Oulmassov

发明人： Qi Wang ,  Patrice Dubois ,  Jihong Liang ,  Tim Oulmassov

IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C12N5/04

CPC分类号： C07K14/415 ,  C12N15/8216 ,  C12N15/8234

摘要： The present invention discloses Arcelin promoters capable of transcribing a heterologous nucleic acid sequence at high levels in plants. The promoters are particularly suited for use in soybean plants and plant cells. Methods of modifying, producing, and using the promoters are also disclosed. The invention further discloses compositions, transformed host cells, transgenic plants, and seeds containing the high-expression Arcelin promoters, and methods for preparing and using the same.

公开(公告)号：US20060282922A1

公开(公告)日：2006-12-14

申请号：US11436903

申请日：2006-05-18

申请人： Qi Wang ,  Tanya Fagaly ,  Ronald Bassuner ,  Jihong Liang ,  Tim Oulmassov ,  John Dabrowski

发明人： Qi Wang ,  Tanya Fagaly ,  Ronald Bassuner ,  Jihong Liang ,  Tim Oulmassov ,  John Dabrowski

IPC分类号： C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8254

摘要： The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.

公开(公告)号：US20070180570A1

公开(公告)日：2007-08-02

申请号：US11625709

申请日：2007-01-22

申请人： Qi Wang ,  Lisa Weaver ,  Tim Oulmassov ,  Jeffrey Ahrens ,  Patrice Dubois ,  Jeffery Shen

发明人： Qi Wang ,  Lisa Weaver ,  Tim Oulmassov ,  Jeffrey Ahrens ,  Patrice Dubois ,  Jeffery Shen

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8234 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8254

摘要： The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression during a defined period of embryogenesis. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.

摘要翻译： 本发明涉及植物遗传工程领域。 更具体地，本发明涉及在胚胎发生的确定时期内的种子特异性基因表达。 本发明提供能够转录种子中的异源核酸序列的启动子，以及修饰，产生和使用它们的方法。

公开(公告)号：US20050227285A1

公开(公告)日：2005-10-13

申请号：US11145451

申请日：2005-06-03

申请人： Tim Oulmassov ,  Kevin McBride ,  Paula Miller ,  John Anderson ,  Lyle Crossland ,  Thomas Adams ,  Barbara Qurollo ,  Victoria Gavrias

发明人： Tim Oulmassov ,  Kevin McBride ,  Paula Miller ,  John Anderson ,  Lyle Crossland ,  Thomas Adams ,  Barbara Qurollo ,  Victoria Gavrias

IPC分类号： A01H5/00 ,  A01K67/027 ,  A61K31/7088 ,  A61K48/00 ,  A61P31/00 ,  C07D307/33 ,  C07H21/04 ,  C12N1/19 ,  C12N5/04 ,  C12N5/10 ,  C12N9/18 ,  C12N15/09 ,  C12N15/81 ,  C12N15/82 ,  C12N15/85 ,  C12Q1/02 ,  C12Q1/68

CPC分类号： C07D307/33 ,  A61K48/00 ,  C12N15/81 ,  C12N15/8214 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8238 ,  C12N15/85 ,  C12N2830/00 ,  C12N2830/001 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2830/30 ,  C12N2830/36 ,  C12N2840/105 ,  C12N2840/20 ,  C12N2840/203

摘要： DNA constructs and other compositions and methods for controlling gene expression in eukaryotic cells and organisms are derived from bacterial quorum sensing systems. One or more cis elements from the luxI promoter (“lux box”) or a functionally similar sequence are incorporated in a eukaryotic promoter. A receptor protein from the LuxR family of transcriptional regulators, upon binding an acylated homoserine lactone (AHL) compound, interacts with the lux box, modulating the activity of the promoter.