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公开(公告)号：US20100041610A1
公开(公告)日：2010-02-18
申请号：US12581449
申请日：2009-10-19
申请人： Timothy Denver Hey , Thomas Meade , Stephanie Love Burton , Donald Joseph Merlo , Qihua Cai , Haley Jo Moon , Joel Jay Sheets , Aaron Todd Woosley
发明人： Timothy Denver Hey , Thomas Meade , Stephanie Love Burton , Donald Joseph Merlo , Qihua Cai , Haley Jo Moon , Joel Jay Sheets , Aaron Todd Woosley
CPC分类号： C12N15/8286 , C07K14/195 , C07K14/24 , C07K14/32 , C07K2319/00 , C07K2319/55 , C12N15/62 , Y02A40/162
摘要： The subject invention relates to insecticidal toxin complex (“TC”) fusion proteins and to polynucleotides that encode these fusion proteins. In some embodiments, the invention provides a fusion protein comprising a Class A protein, a Class B protein, and a Class C TC protein fused together to form a single protein. In some other embodiments, the invention provides a fusion protein comprising a Class B and a Class C TC proteins fused together. In the latter embodiments, the BC or CB fusion protein can be used to enhance or potentiate the anti-insect activity of a “Toxin A” or Class A protein. The subject invention also includes plants, cells (bacterial and plant cells for example), and seeds that comprise the polynucleotides. The subject invention also includes methods of controlling pests (preferably insects and other plant pests) with fusion proteins of the subject invention.
摘要翻译： 本发明涉及杀虫毒素复合物（“TC”）融合蛋白和编码这些融合蛋白的多核苷酸。 在一些实施方案中，本发明提供了融合蛋白，其包含一起融合以形成单一蛋白质的A类蛋白质，B类蛋白质和C类TC蛋白质。 在一些其他实施方案中，本发明提供了融合蛋白，其包含融合在一起的B类和C类TC蛋白。 在后一个实施方案中，BC或CB融合蛋白可用于增强或增强“毒素A”或A类蛋白的抗昆虫活性。 本发明还包括植物，细胞（例如细菌和植物细胞）和包含多核苷酸的种子。 本发明还包括用本发明的融合蛋白控制害虫（优选昆虫和其它植物害虫）的方法。
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公开(公告)号：US07605228B2
公开(公告)日：2009-10-20
申请号：US11070573
申请日：2005-03-02
申请人： Timothy Denver Hey , Thomas Meade , Stephanie Love Burton , Donald Joseph Merlo , Qihua Cai , Haley Jo Moon , Joel Jay Sheets , Aaron Todd Woosley
发明人： Timothy Denver Hey , Thomas Meade , Stephanie Love Burton , Donald Joseph Merlo , Qihua Cai , Haley Jo Moon , Joel Jay Sheets , Aaron Todd Woosley
CPC分类号： C12N15/8286 , C07K14/195 , C07K14/24 , C07K14/32 , C07K2319/00 , C07K2319/55 , C12N15/62 , Y02A40/162
摘要： The subject invention relates to insecticidal toxin complex (“TC”) fusion proteins and to polynucleotides that encode these fusion proteins. In some embodiments, the invention provide a fusion protein comprising a Class A protein, a Class B protein, and a Class C TC protein fused together to form a single protein. In some other embodiments, the invention provides a fusion protein compromising a Class B and a Class C TC proteins fused together. In the latter embodiments, the BC and CB fusion protein can be used to enhance or potentate the anti-insect activity of a “Toxin A” or Class A protein. The subject invention also includes plants, cells (bacterial and plant cells for example) and seeds that comprise the polynueleotides. The subject invention also includes methods of controlling pests (preferably insects and other plant pests) with fusion proteins of the subject invention.
摘要翻译： 本发明涉及杀虫毒素复合物（“TC”）融合蛋白和编码这些融合蛋白的多核苷酸。 在一些实施方案中，本发明提供了包含A类蛋白，B类蛋白和C类TC蛋白的融合蛋白，其融合在一起以形成单一蛋白。 在一些其它实施方案中，本发明提供了融合蛋白质，并融合在一起的B类和C类TC蛋白质。 在后一个实施方案中，BC和CB融合蛋白可用于增强或增强“毒素A”或A类蛋白的抗昆虫活性。 本发明还包括植物，细胞（例如细菌和植物细胞）和包含多核苷酸的种子。 本发明还包括用本发明的融合蛋白控制害虫（优选昆虫和其它植物害虫）的方法。
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3.发明授权Sources for, and types of, insecticidally active proteins, and polynucleotides that encode the proteins 有权杀虫活性蛋白质的来源和类型，以及编码蛋白质的多核苷酸公开(公告)号：US07812131B2
公开(公告)日：2010-10-12
申请号：US11366918
申请日：2006-03-02
申请人： Ignacio Mario Larrinua , Aaron Todd Woosley , Thomas Meade , Donald Joseph Merlo , Timothy Denver Hey , Stephanie Love Burton
发明人： Ignacio Mario Larrinua , Aaron Todd Woosley , Thomas Meade , Donald Joseph Merlo , Timothy Denver Hey , Stephanie Love Burton
IPC分类号： C07K14/415 , A61K38/56 , A61K38/00
CPC分类号： C07K14/195 , A01N63/00 , A01N63/04 , C07K14/20 , C07K14/21 , C07K14/37 , C07K16/14 , C07K2319/00 , Y10S530/858
摘要： The subject invention provides exciting new sources for surprising, new types of toxin complex (“TC”) proteins. The subject invention includes these new classes and types of TC proteins. The subject invention also includes polynucleotides that encode the subject proteins. The subject invention further provides vectors and cells comprising these polynucleotides. The subject invention also provides novel methods of controlling insects. The subject invention relates in part to the surprising discovery that new types of TC proteins can be obtained from a widely diverse phylogenetic spectrum of organisms including, most notably and surprisingly, eukaryotic fungus.
摘要翻译： 本发明为令人惊奇的新型毒素复合物（“TC”）蛋白质提供令人兴奋的新来源。 本发明包括这些新类型和TC蛋白。 本发明还包括编码受试蛋白的多核苷酸。 本发明进一步提供了包含这些多核苷酸的载体和细胞。 本发明还提供了控制昆虫的新方法。 本发明部分地涉及令人惊奇的发现，即可以从广泛多样的生物系统发育谱获得新型TC蛋白质，包括最显着和令人惊讶的真核真菌。
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4.发明申请Use of untranslated region of osmotin gene to enhance transgene expression in plants 有权使用非翻译区的osmotin基因来增强植物中的转基因表达公开(公告)号：US20090064376A1
公开(公告)日：2009-03-05
申请号：US12012813
申请日：2008-02-05
CPC分类号： C12N15/8216 , C07H21/04
摘要： The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5′ and 3′ untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.
摘要翻译： 本发明提供用于增强转基因植物和植物组织中重组核酸序列表达的方法，载体和基因构建体。 根据本发明，获得核酸序列和/或衍生自编码osmotin蛋白的基因的5'和3'非翻译区，并被工程改造成位于载体的选定编码区的相应部分。 载体构建体可以通过常规方法引入植物和/或植物组织中，导致所选编码区的表达增强。 在优选的实施方案中，选择的编码区是表达一种或多种已知赋予转基因植物和/或植物组织杀虫活性水平的蛋白质的嵌合基因或基因片段。
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5.发明授权Use of untranslated region of osmotin gene to enhance transgene expression in plants 有权使用非翻译区的osmotin基因来增强植物中的转基因表达公开(公告)号：US08008466B2
公开(公告)日：2011-08-30
申请号：US11448061
申请日：2006-06-06
IPC分类号： C07H21/04 , C12N15/00 , C12N15/113 , C12N15/63
CPC分类号： C12N15/8216 , C07H21/04
摘要： The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5′ and 3′ untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.
摘要翻译： 本发明提供用于增强转基因植物和植物组织中重组核酸序列表达的方法，载体和基因构建体。 根据本发明，获得核酸序列和/或衍生自编码osmotin蛋白的基因的5'和3'非翻译区，并被工程改造成位于载体的选定编码区的相应部分。 载体构建体可以通过常规方法引入植物和/或植物组织中，导致所选编码区的表达增强。 在优选的实施方案中，选择的编码区是表达一种或多种已知赋予转基因植物和/或植物组织杀虫活性水平的蛋白质的嵌合基因或基因片段。
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