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    METHOD TO PRODUCE ACETYLDIACYLGLYCEROLS (AC-TAGS) BY EXPRESSION OF AN ACETYLTRANSFERASE GENE ISOLATED FROM EUONYMUS ALATUS (BURNING BUSH)
    1.
    发明申请
    METHOD TO PRODUCE ACETYLDIACYLGLYCEROLS (AC-TAGS) BY EXPRESSION OF AN ACETYLTRANSFERASE GENE ISOLATED FROM EUONYMUS ALATUS (BURNING BUSH) 有权
    通过从EUONYMUS ALATUS（BURNING BUSH）分离出的乙酰胆碱酯酶基因的表达来生产乙酰胆碱酯（AC-标记）的方法

    公开(公告)号：US20130116462A1

    公开(公告)日：2013-05-09

    申请号：US13519660

    申请日：2010-12-29

    申请人： Timothy Durrett ,  John Ohlrogge ,  Michael Pollard

    发明人： Timothy Durrett ,  John Ohlrogge ,  Michael Pollard

    IPC分类号： C12N9/10 ,  C12P7/64 ,  C12N15/82

    CPC分类号： C12N9/1029 ,  C12N15/8247 ,  C12P7/6463 ,  Y02T50/678

    摘要： The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories.

    摘要翻译： 本发明涉及新的二酰基甘油酰基转移酶基因和蛋白质及其使用方法。 特别地，本发明描述了编码具有二酰基甘油乙酰转移酶活性的蛋白质的基因，特别用于将乙酰基转移到二酰基甘油底物以形成乙酰基三酰基甘油（ac-TAGS），例如3-乙酰基-1,2-二酰基-sn 甘油。 本发明包括转移酶的天然和重组野生型形式，以及突变体和变体形式。 本发明还涉及使用新的二酰基甘油酰基转移酶基因和蛋白质的方法，包括在商业上可行的水平上在转基因生物体中的表达，用于增加植物油中3-乙酰基-1,2-二酰基-sn-甘油的生产并改变 通过增加ac-TAG生产，通过微生物如酵母生产的油的组成。 此外，通过包含基因和蛋白质的本发明的方法产生的油被考虑用作生物柴油燃料，在聚合物生产中和作为具有降低的热量的天然产生的食用油。

    Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)
    2.
    发明授权
    Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush) 有权
    通过从卫矛（燃烧丛）分离的乙酰转移酶基因的表达产生乙酰二酰基甘油（ac-TAG）的方法

    公开(公告)号：US09328335B2

    公开(公告)日：2016-05-03

    申请号：US13519660

    申请日：2010-12-29

    申请人： Timothy Durrett ,  John Ohlrogge ,  Michael Pollard

    发明人： Timothy Durrett ,  John Ohlrogge ,  Michael Pollard

    IPC分类号： C12N9/10 ,  C12N15/82 ,  C12P7/64

    CPC分类号： C12N9/1029 ,  C12N15/8247 ,  C12P7/6463 ,  Y02T50/678

    摘要： The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories.

    摘要翻译： 本发明涉及新的二酰基甘油酰基转移酶基因和蛋白质及其使用方法。 特别地，本发明描述了编码具有二酰基甘油乙酰转移酶活性的蛋白质的基因，特别用于将乙酰基转移到二酰基甘油底物以形成乙酰基三酰基甘油（ac-TAGS），例如3-乙酰基-1,2-二酰基-sn 甘油。 本发明包括转移酶的天然和重组野生型形式，以及突变体和变体形式。 本发明还涉及使用新的二酰基甘油酰基转移酶基因和蛋白质的方法，包括在商业上可行的水平上在转基因生物体中的表达，用于增加植物油中3-乙酰基-1,2-二酰基-sn-甘油的生产并改变 通过增加ac-TAG生产，通过微生物如酵母生产的油的组成。 此外，通过包含基因和蛋白质的本发明的方法产生的油被考虑用作生物柴油燃料，在聚合物生产中和作为具有降低的热量的天然产生的食用油。

    Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof
    3.
    发明申请
    Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof 失效
    植物环丙烷脂肪酸合成酶基因，蛋白质及其用途

    公开(公告)号：US20060053512A1

    公开(公告)日：2006-03-09

    申请号：US10499246

    申请日：2002-12-20

    申请人： Xiaoming Bao ,  John Ohlrogge ,  Michael Pollard

    发明人： Xiaoming Bao ,  John Ohlrogge ,  Michael Pollard

    IPC分类号： A01H1/00 ,  C12N15/87 ,  C07H21/04 ,  C12N9/12

    CPC分类号： C12N9/1007 ,  C12N15/8247

    摘要： The present invention provides cyclopropane fatty acid synthase genes and proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also provides methods of using cyclopropane fatty acid synthase genes and proteins, including in their expression in transgenic organisms and in the production of cyclopropane fatty acids in plant oils, and in particular seed oils.

    摘要翻译： 本发明提供了环丙烷脂肪酸合酶基因和蛋白质及其使用方法。 本发明包括合成酶的天然和重组野生型形式，以及突变体和变体形式，其中一些相对于野生型合酶具有改变的特征。 本发明还提供了使用环丙烷脂肪酸合酶基因和蛋白质的方法，包括在转基因生物体中的表达和在植物油中特别是种子油中的环丙烷脂肪酸的生产。

    Compositions and methods for using acyltransferases for altering lipid production on the surface of plants
    6.
    发明申请
    Compositions and methods for using acyltransferases for altering lipid production on the surface of plants 审中-公开
    使用酰基转移酶改变植物表面脂质生成的组合物和方法

    公开(公告)号：US20090163729A1

    公开(公告)日：2009-06-25

    申请号：US11821182

    申请日：2007-06-22

    申请人： Yonghua Li ,  Fred Beisson ,  Mike Pollard ,  John Ohlrogge

    发明人： Yonghua Li ,  Fred Beisson ,  Mike Pollard ,  John Ohlrogge

    IPC分类号： C11B1/00 ,  C12N15/63 ,  A01H5/00 ,  C12N15/82

    CPC分类号： C12N9/1029 ,  C12N15/8247

    摘要： The present invention relates to compositions comprising acyltransferase nucleic acid molecules for altering lipids on the surface of plants, and related methods. In particular, the present invention provides compositions and methods for increasing the amount of free fatty acids, acylglycerols, and other lipids on the surface of a plant. In a preferred embodiment, the present invention relates to increasing activity of a GPAT acyltransferase for altering lipid on the plant surface, for increasing surface lipids, for enhancing environmental stress tolerance, increasing resistance to biotic stress, and providing novel plant lipids for commercial products. In further embodiments, the present invention relates to using an Arabidopsis thaliana GPAT acyltransferase for altering lipid compounds on the surface of a plant.

    摘要翻译： 本发明涉及包含用于改变植物表面脂质的酰基转移酶核酸分子的组合物及相关方法。 特别地，本发明提供了用于增加植物表面上的游离脂肪酸，酰基甘油和其它脂质的量的组合物和方法。 在优选的实施方案中，本发明涉及增加GPAT酰基转移酶的活性，以改变植物表面上的脂质，增加表面脂质，增强环境胁迫耐受性，增加对生物胁迫的抗性，以及为商业产品提供新的植物脂质。 在另外的实施方案中，本发明涉及使用拟南芥GPAT酰基转移酶来改变植物表面上的脂质化合物。