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    METHOD TO PRODUCE ACETYLDIACYLGLYCEROLS (AC-TAGS) BY EXPRESSION OF AN ACETYLTRANSFERASE GENE ISOLATED FROM EUONYMUS ALATUS (BURNING BUSH)
    1.
    发明申请
    METHOD TO PRODUCE ACETYLDIACYLGLYCEROLS (AC-TAGS) BY EXPRESSION OF AN ACETYLTRANSFERASE GENE ISOLATED FROM EUONYMUS ALATUS (BURNING BUSH) 有权
    通过从EUONYMUS ALATUS（BURNING BUSH）分离出的乙酰胆碱酯酶基因的表达来生产乙酰胆碱酯（AC-标记）的方法

    公开(公告)号：US20130116462A1

    公开(公告)日：2013-05-09

    申请号：US13519660

    申请日：2010-12-29

    申请人： Timothy Durrett ,  John Ohlrogge ,  Michael Pollard

    发明人： Timothy Durrett ,  John Ohlrogge ,  Michael Pollard

    IPC分类号： C12N9/10 ,  C12P7/64 ,  C12N15/82

    CPC分类号： C12N9/1029 ,  C12N15/8247 ,  C12P7/6463 ,  Y02T50/678

    摘要： The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories.

    摘要翻译： 本发明涉及新的二酰基甘油酰基转移酶基因和蛋白质及其使用方法。 特别地，本发明描述了编码具有二酰基甘油乙酰转移酶活性的蛋白质的基因，特别用于将乙酰基转移到二酰基甘油底物以形成乙酰基三酰基甘油（ac-TAGS），例如3-乙酰基-1,2-二酰基-sn 甘油。 本发明包括转移酶的天然和重组野生型形式，以及突变体和变体形式。 本发明还涉及使用新的二酰基甘油酰基转移酶基因和蛋白质的方法，包括在商业上可行的水平上在转基因生物体中的表达，用于增加植物油中3-乙酰基-1,2-二酰基-sn-甘油的生产并改变 通过增加ac-TAG生产，通过微生物如酵母生产的油的组成。 此外，通过包含基因和蛋白质的本发明的方法产生的油被考虑用作生物柴油燃料，在聚合物生产中和作为具有降低的热量的天然产生的食用油。

    Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)
    2.
    发明授权
    Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush) 有权
    通过从卫矛（燃烧丛）分离的乙酰转移酶基因的表达产生乙酰二酰基甘油（ac-TAG）的方法

    公开(公告)号：US09328335B2

    公开(公告)日：2016-05-03

    申请号：US13519660

    申请日：2010-12-29

    申请人： Timothy Durrett ,  John Ohlrogge ,  Michael Pollard

    发明人： Timothy Durrett ,  John Ohlrogge ,  Michael Pollard

    IPC分类号： C12N9/10 ,  C12N15/82 ,  C12P7/64

    CPC分类号： C12N9/1029 ,  C12N15/8247 ,  C12P7/6463 ,  Y02T50/678

    摘要： The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories.

    摘要翻译： 本发明涉及新的二酰基甘油酰基转移酶基因和蛋白质及其使用方法。 特别地，本发明描述了编码具有二酰基甘油乙酰转移酶活性的蛋白质的基因，特别用于将乙酰基转移到二酰基甘油底物以形成乙酰基三酰基甘油（ac-TAGS），例如3-乙酰基-1,2-二酰基-sn 甘油。 本发明包括转移酶的天然和重组野生型形式，以及突变体和变体形式。 本发明还涉及使用新的二酰基甘油酰基转移酶基因和蛋白质的方法，包括在商业上可行的水平上在转基因生物体中的表达，用于增加植物油中3-乙酰基-1,2-二酰基-sn-甘油的生产并改变 通过增加ac-TAG生产，通过微生物如酵母生产的油的组成。 此外，通过包含基因和蛋白质的本发明的方法产生的油被考虑用作生物柴油燃料，在聚合物生产中和作为具有降低的热量的天然产生的食用油。

    Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof
    3.
    发明申请
    Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof 失效
    植物环丙烷脂肪酸合成酶基因，蛋白质及其用途

    公开(公告)号：US20060053512A1

    公开(公告)日：2006-03-09

    申请号：US10499246

    申请日：2002-12-20

    申请人： Xiaoming Bao ,  John Ohlrogge ,  Michael Pollard

    发明人： Xiaoming Bao ,  John Ohlrogge ,  Michael Pollard

    IPC分类号： A01H1/00 ,  C12N15/87 ,  C07H21/04 ,  C12N9/12

    CPC分类号： C12N9/1007 ,  C12N15/8247

    摘要： The present invention provides cyclopropane fatty acid synthase genes and proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also provides methods of using cyclopropane fatty acid synthase genes and proteins, including in their expression in transgenic organisms and in the production of cyclopropane fatty acids in plant oils, and in particular seed oils.

    摘要翻译： 本发明提供了环丙烷脂肪酸合酶基因和蛋白质及其使用方法。 本发明包括合成酶的天然和重组野生型形式，以及突变体和变体形式，其中一些相对于野生型合酶具有改变的特征。 本发明还提供了使用环丙烷脂肪酸合酶基因和蛋白质的方法，包括在转基因生物体中的表达和在植物油中特别是种子油中的环丙烷脂肪酸的生产。

    Leg exercise device
    4.
    发明授权
    Leg exercise device 失效
    腿部锻炼装置

    公开(公告)号：US5221242A

    公开(公告)日：1993-06-22

    申请号：US725026

    申请日：1991-07-03

    申请人： James C. Weber ,  Clayton L. Nannen ,  Michael Pollard ,  Donald R. Pollard

    发明人： James C. Weber ,  Clayton L. Nannen ,  Michael Pollard ,  Donald R. Pollard

    IPC分类号： A63B21/015 ,  A63B22/14 ,  A63B23/00 ,  A63B23/035

    CPC分类号： A63B23/08 ,  A63B21/015 ,  A63B22/14 ,  A63B2023/003 ,  A63B2208/0233

    摘要： A device for exercising internal and external muscles of the leg is provided. The device includes a foot holder for pivotally supporting a user's foot. The foot holder is capable of pivoting around an axis which is substantially perpendicular to the foot, and near the front of the user's leg. The device also includes a tension control unit for providing constant resistance to pivoting of the foot holder. The device preferably has separate foot holders and hydraulic tension control units for each foot.

    摘要翻译： 提供了用于行使腿部的内部和外部肌肉的装置。 该装置包括用于枢转地支撑使用者的脚的脚架。 脚架能够围绕基本上垂直于脚并且靠近使用者腿部的前部的轴线枢转。 该装置还包括张力控制单元，用于提供对脚架的枢转的恒定阻力。 该装置优选地具有用于每个脚的单独的脚架和液压张力控制单元。

    Catalytic converter
    6.
    发明申请
    Catalytic converter 审中-公开
    催化转化器

    公开(公告)号：US20070122318A1

    公开(公告)日：2007-05-31

    申请号：US11289213

    申请日：2005-11-29

    申请人： Craig Habeger ,  Michael Pollard

    发明人： Craig Habeger ,  Michael Pollard

    IPC分类号： B01D50/00 ,  B21D51/16

    CPC分类号： F01N3/2814 ,  F01N3/2821 ,  F01N2330/02 ,  Y10T29/49345

    摘要： A catalytic converter, an exhaust system comprising a catalytic converter, and a method of manufacturing a catalytic converter are provided. The catalytic converter comprises at least one channel configured to receive gas flow and a catalyst coated on the at least one channel. In one of the disclosed embodiments, at least one channel is partially non-linear along its length.

    摘要翻译： 提供催化转化器，包括催化转化器的排气系统和制造催化转化器的方法。 催化转化器包括构造成接收气流的至少一个通道和涂覆在至少一个通道上的催化剂。 在所公开的实施例之一中，至少一个通道沿其长度部分是非线性的。

    Electrical connection for porous material
    9.
    发明申请
    Electrical connection for porous material 失效
    电气连接多孔材料

    公开(公告)号：US20060021303A1

    公开(公告)日：2006-02-02

    申请号：US10902290

    申请日：2004-07-30

    申请人： Matthew Williams ,  Matthew Kiser ,  Michael Pollard ,  Jo Costura ,  Cornelius Opris ,  Hind Abi-Akar ,  Robert Meyer

    发明人： Matthew Williams ,  Matthew Kiser ,  Michael Pollard ,  Jo Costura ,  Cornelius Opris ,  Hind Abi-Akar ,  Robert Meyer

    IPC分类号： B01D46/00

    CPC分类号： H01R13/533 ,  F01N3/027 ,  H01R4/64

    摘要： An electrical connection element for providing an electrical connection to a porous material may include a first electrically conductive plate disposed on at least a portion of a first side of the porous material. A second electrically conductive plate may be disposed on at least a portion of a second side of the porous material, opposite to the first side. An electrically conductive material may impregnate the porous material in a region between the first and second electrically conductive plates, and an electrical connector may be attached to at least one of the first and second electrically conductive plates.

    摘要翻译： 用于提供与多孔材料的电连接的电连接元件可以包括设置在多孔材料的第一侧的至少一部分上的第一导电板。 第二导电板可以设置在多孔材料的与第一侧相对的第二侧的至少一部分上。 导电材料可以在第一和第二导电板之间的区域中浸渍多孔材料，并且电连接器可以附接到第一和第二导电板中的至少一个。

    ORIENTABLE WHIPSTOCK TOOL AND METHOD
    10.
    发明申请
    ORIENTABLE WHIPSTOCK TOOL AND METHOD 有权
    方便的工具和方法

    公开(公告)号：US20050145381A1

    公开(公告)日：2005-07-07

    申请号：US10668731

    申请日：2003-09-23

    申请人： Michael Pollard

    发明人： Michael Pollard

    IPC分类号： E21B7/06

    CPC分类号： E21B7/061

    摘要： A whipstock assembly includes a whipstock body 8 having a whipstock center of gravity offset radially from the central axis of the whipstock body, a counterweight to releasably secured to the whipstock body, with a counterweight having an offset counterweight center of gravity, an orientation device 5, and a neutralizer 11. The neutralizer causes the combined whipstock/neutralizer center of gravity to be substantially closer to the central axis of the whipstock body compared to the whipstock center of gravity. An attaching mechanism 6, 12 allows the neutralizer to be returned to the surface after the whipstock body is set in the well.

    摘要翻译： 造斜器组件包括一个造斜器主体8，其具有从造斜器主体的中心轴线径向偏移的造斜器重心，配重可释放地固定到造斜器主体，配重具有偏置配重重心，定向装置5 和中和器11.中和器使得组合的造斜器/中和器重心基本上比造斜机重心更接近造斜器主体的中心轴线。 安装机构6,12允许在造斜器主体被放置在井中之后将中和器返回到表面。