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公开(公告)号：US20100206730A1
公开(公告)日：2010-08-19
申请号：US12562070
申请日：2009-09-17
IPC分类号： G01N27/26
CPC分类号： G01N27/44726
摘要： The present teachings relate, among other things, to polynucleotide sequencing, fragment analysis and sample/lane tracking, and to polynucleotide sequencers and analyzers that employ optical detection techniques. Embodiments of the present teachings are described which include, for example, the addition of a calibration standard to a sequencing reaction. Information such as peak spacing and peak shape can be extracted from the standard.
摘要翻译： 本教导涉及多核苷酸测序，片段分析和样品/泳道跟踪，以及使用光学检测技术的多核苷酸测序仪和分析仪。 描述了本教导的实施例，其包括例如向测序反应添加校准标准。 可以从标准中提取峰间距和峰形等信息。
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公开(公告)号：US08268558B2
公开(公告)日：2012-09-18
申请号：US12562070
申请日：2009-09-17
CPC分类号： G01N27/44726
摘要： The present teachings relate, among other things, to polynucleotide sequencing, fragment analysis and sample/lane tracking, and to polynucleotide sequencers and analyzers that employ optical detection techniques. Embodiments of the present teachings are described which include, for example, the addition of a calibration standard to a sequencing reaction. Information such as peak spacing and peak shape can be extracted from the standard.
摘要翻译： 本教导涉及多核苷酸测序，片段分析和样品/泳道跟踪，以及使用光学检测技术的多核苷酸测序仪和分析仪。 描述了本教导的实施例，其包括例如向测序反应添加校准标准。 可以从标准中提取峰间距和峰形等信息。
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公开(公告)号：US07593819B2
公开(公告)日：2009-09-22
申请号：US10193776
申请日：2002-07-11
CPC分类号： G01N27/44726
摘要： The present teachings relate, among other things, to polynucleotide sequencing, fragment analysis and sample/lane tracking, and to polynucleotide sequencers and analyzers that employ optical detection techniques. Embodiments of the present teachings are described which include, for example, the addition of a calibration standard to a sequencing reaction. Information such as peak spacing and peak shape can he extracted from the standard.
摘要翻译： 本教导涉及多核苷酸测序，片段分析和样品/泳道跟踪，以及使用光学检测技术的多核苷酸测序仪和分析仪。 描述了本教导的实施例，其包括例如向测序反应添加校准标准。 可以从标准中提取峰间距和峰形等信息。
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公开(公告)号：US20050059046A1
公开(公告)日：2005-03-17
申请号：US10871081
申请日：2004-06-18
申请人： James LaBrenz , Jon Sorenson , Curtis Gehman
发明人： James LaBrenz , Jon Sorenson , Curtis Gehman
CPC分类号： G16B30/00 , G01N27/44721 , G16B40/00
摘要： Nucleic acid sequence determination is a method whereby peaks in data traces representing the detection of labeled nucleotides are classified as either noise or specific nucleotides. Embodiments are described herein that formulate this classification as a graph theory problem whereby graph edges encode peak characteristics. The graph can then be traversed to find the shortest path. Various embodiments formulate the graph in such a way as to minimize computational time. In various cases it is desirable that such classification allow for the possibility of mixed bases in the nucleotide sequence. Embodiments are described herein that address the classification of mixed-bases. Embodiments are also described that detail methods and systems for processing the data in order to make the classification step robust and reliable.
摘要翻译： 核酸序列测定是将代表标记的核苷酸检测的数据踪迹中的峰分类为噪声或特定核苷酸的方法。 本文描述了将该分类制定为图论的问题，其中图形边缘编码峰值特性。 然后可以遍历图形以找到最短路径。 各种实施例以使得计算时间最小化的方式来制定图形。 在各种情况下，希望这种分类允许在核苷酸序列中混合碱基的可能性。 本文描述了解混合基底的分类的实施例。 还描述了用于处理数据以便使分类步骤稳健和可靠的详细方法和系统的实施例。
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