Internal Calibration Standards for Electrophoretic Analyses
    3.
    发明申请
    Internal Calibration Standards for Electrophoretic Analyses 有权
    电泳分析的内部校准标准

    公开(公告)号:US20100206730A1

    公开(公告)日:2010-08-19

    申请号:US12562070

    申请日:2009-09-17

    IPC分类号: G01N27/26

    CPC分类号: G01N27/44726

    摘要: The present teachings relate, among other things, to polynucleotide sequencing, fragment analysis and sample/lane tracking, and to polynucleotide sequencers and analyzers that employ optical detection techniques. Embodiments of the present teachings are described which include, for example, the addition of a calibration standard to a sequencing reaction. Information such as peak spacing and peak shape can be extracted from the standard.

    摘要翻译: 本教导涉及多核苷酸测序,片段分析和样品/泳道跟踪,以及使用光学检测技术的多核苷酸测序仪和分析仪。 描述了本教导的实施例,其包括例如向测序反应添加校准标准。 可以从标准中提取峰间距和峰形等信息。

    METHOD OF GENERATING LONG NUCLEIC ACID MOLECULES OF DEFINED SEQUENCE
    6.
    发明申请
    METHOD OF GENERATING LONG NUCLEIC ACID MOLECULES OF DEFINED SEQUENCE 有权
    产生定义序列的长核酸分子的方法

    公开(公告)号:US20070254343A1

    公开(公告)日:2007-11-01

    申请号:US11770787

    申请日:2007-06-29

    IPC分类号: C12P19/34

    CPC分类号: C12N15/1031

    摘要: A method of generating a single-stranded nucleic acid molecule comprising (a) combining in a mixture under conditions suitable for a polymerase extension reaction, (i) a polymerase, (ii) an initial polynucleotide comprising a 5′ portion and a 3′ portion, wherein the polynucleotide forms the nucleic acid molecule 5′ end; and (iii) a plurality of overlapping template oligonucleotides each having a 5′ template portion and a 3′ portion. The method further comprises (b) hybridizing the initial polynucleotide or the extension polynucleotide and one of the template oligonucleotides; (c) incubating the mixture for sufficient time to allow an extension polynucleotide to be synthesized; (d) adding a competimer that competes with the template oligonucleotide in step (b); (e) denaturing the extension polynucleotide and template oligonucleotide; and (f) repeating steps (b), (c), (d), and (e) to generate the single-stranded nucleic acid molecule, wherein the number of repeated cycles equals the number of different template oligonucleotides.

    摘要翻译: 一种产生单链核酸分子的方法,包括(a)在适于聚合酶延伸反应的条件下在混合物中混合,(i)聚合酶,(ii)包含5'部分和3'部分的初始多核苷酸 ,其中所述多核苷酸形成核酸分子5'末端; 和(iii)多个具有5'模板部分和3'部分的重叠模板寡核苷酸。 该方法还包括(b)使初始多核苷酸或延伸多核苷酸与模板寡核苷酸之一杂交; (c)将混合物温育足够的时间以允许合成延伸的多核苷酸; (d)在步骤(b)中加入与模板寡核苷酸竞争的竞争体; (e)使延伸多核苷酸和模板寡核苷酸变性; 和(f)重复步骤(b),(c),(d)和(e)以产生单链核酸分子,其中重复循环的数量等于不同模板寡核苷酸的数目。

    Methods and compositions for improving the loading of analytical instruments
    7.
    发明授权
    Methods and compositions for improving the loading of analytical instruments 有权
    提高分析仪器负荷的方法和组成

    公开(公告)号:US06168701A

    公开(公告)日:2001-01-02

    申请号:US09303789

    申请日:1999-04-30

    IPC分类号: G01N2726

    摘要: The invention relates to improved methods and compositions for loading samples into an analytical instrument having a plurality of sample receiving loading ports. In a principle embodiment of the invention, first and second sample markers are added to sample to be loaded onto an analytical instrument having a plurality of sample receiving loading ports. The first and second samples are compounds are selected so as to produce a distinctive signal upon combination thus a sample containing a first sample marker and a sample containing a second marker are mistakenly loaded into the same sample receiving loading port, a detectable signal indicative of the misloading is produced.

    摘要翻译: 本发明涉及用于将样品装载到具有多个样品接收加载端口的分析仪器中的改进的方法和组合物。 在本发明的主要实施例中,将第一和第二样品标记物添加到待加载到具有多个样品接收加载端口的分析仪器上的样品中。 选择第一和第二样品是为了在组合时产生独特的信号,因此含有第一样品标记物和含有第二标记物的样品的样品被错误地加载到相同的样品接收加载端口中,可检测信号指示 产生错位。

    Methods for the analysis of proximity binding assay data

    公开(公告)号:US10208335B2

    公开(公告)日:2019-02-19

    申请号:US12851532

    申请日:2010-08-05

    摘要: Various embodiments of methods for analyzing proximity binding assay (PBA) data are disclosed. Proximity binding assays as a class of analyses offer the advantages of the sensitivity and specificity of biorecognition binding, along with the exponential signal amplification offered by a variety of oligonucleotide amplification reactions, such as the polymerase chain reaction (PCR). However, as various proximity binding assays have reaction kinetics governed by an additional step of the binding of a biorecognition probe (BRP) with a target molecule, there is a need for methods for the analysis of PBA data that are particularly suited to the unique characteristics of such data.

    Methods for the Analysis of Proximity Binding Assay Data
    9.
    发明申请
    Methods for the Analysis of Proximity Binding Assay Data 审中-公开
    接近结合测定数据分析方法

    公开(公告)号:US20110208441A1

    公开(公告)日:2011-08-25

    申请号:US12851532

    申请日:2010-08-05

    IPC分类号: G06F19/00

    摘要: Various embodiments of methods for analyzing proximity binding assay (PBA) data are disclosed. Proximity binding assays as a class of analyses offer the advantages of the sensitivity and specificity of biorecognition binding, along with the exponential signal amplification offered by a variety of oligonucleotide amplification reactions, such as the polymerase chain reaction (PCR). However, as various proximity binding assays have reaction kinetics governed by an additional step of the binding of a biorecognition probe (BRP) with a target molecule, there is a need for methods for the analysis of PBA data that are particularly suited to the unique characteristics of such data.

    摘要翻译: 公开了用于分析邻近结合测定(PBA)数据的方法的各种实施方案。 作为一类分析的接近结合测定提供了生物识别结合的灵敏度和特异性以及由多种寡核苷酸扩增反应(例如聚合酶链反应(PCR))提供的指数信号扩增的优点。 然而,由于各种邻近结合测定具有由生物识别探针(BRP)与靶分子结合的附加步骤所决定的反应动力学,因此需要分析特别适合于独特特征的PBA数据的方法 的数据。

    Method of generating size standard nucleic acids
    10.
    发明申请
    Method of generating size standard nucleic acids 审中-公开
    产生大小标准核酸的方法

    公开(公告)号:US20050095610A1

    公开(公告)日:2005-05-05

    申请号:US10819657

    申请日:2004-04-07

    摘要: Methods for generating nucleic acid size standards are disclosed. The methods comprise providing a template polynucleotide which comprises periodic sequences of from about 5 to about 50 contiguous nucleotides containing not more than three types of nucleotides and wherein adjacent periodic sequences are separated by a terminator complement nucleotide that differs from the not more than three types of nucleotides, and performing a primer extension reaction on the template polynucleotide in the presence of nucleoside triphosphate molecules and a 3′ terminating nucleoside triphosphate which is complementary to the terminator complement nucleotide.

    摘要翻译: 公开了产生核酸尺寸标准的方法。 所述方法包括提供模板多核苷酸,其包含约5至约50个连续核苷酸的周期性序列,所述连续核苷酸含有不超过3种类型的核苷酸,并且其中相邻的周期性序列由终止子补体核苷酸分开,其不同于不超过三种类型 核苷酸,并且在核苷三磷酸分子和与终止子补体核苷酸互补的3'末端核苷三磷酸存在下对模板多核苷酸进行引物延伸反应。