公开(公告)号：US08372616B2

公开(公告)日：2013-02-12

申请号：US12593184

申请日：2008-03-19

申请人： Tomoki Sando ,  Norie Watanabe ,  Koichiro Gyokusen ,  Eiichiro Fukuzaki ,  Akio Kobayashi ,  Haska Nadirman ,  Purbowasito Wahyu

发明人： Tomoki Sando ,  Norie Watanabe ,  Koichiro Gyokusen ,  Eiichiro Fukuzaki ,  Akio Kobayashi ,  Haska Nadirman ,  Purbowasito Wahyu

IPC分类号： C12N9/10

CPC分类号： C12P17/06 ,  C12N9/00 ,  C12N9/001 ,  C12N9/0069 ,  C12N9/1007 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/52 ,  C12N15/8243

摘要： It is a subject of this invention to isolate a gene group of enzymes involved in the biosynthesis of vitamin E of Para rubber tree, and to determine the base sequence of each gene. According to this invention, genes encoding enzymes involved in the vitamin E biosynthesis were isolated from Para rubber tree and the base sequences of these genes were determined. Since vitamin E is an antioxidant existing in nature, it is expected that transformation of a plant by using the genes obtained in the invention would result in an increase in the vitamin E content of the plant and, in its turn, contribute to the prevention of rubber from aging.

摘要翻译： 本发明的主题是分离参与Para橡胶树维生素E生物合成的基因组，并确定每个基因的碱基序列。 根据本发明，从Para橡胶树中分离参与维生素E生物合成的酶的基因，并测定这些基因的碱基序列。 由于维生素E是自然界中存在的抗氧化剂，预计通过使用本发明中获得的基因转化植物将导致植物的维生素E含量增加，反过来也有助于预防 橡胶老化。

公开(公告)号：US20100218272A1

公开(公告)日：2010-08-26

申请号：US12550863

申请日：2009-08-31

申请人： Yoshihisa Nakazawa ,  Yoko Harada ,  Hirotaka Uefuji ,  Ren Chen ,  Takeshi Bamba ,  Akio Kobayashi ,  Eiichiro Fukusaki ,  Kazumasa Hirata ,  Koichiro Gyokusen

发明人： Yoshihisa Nakazawa ,  Yoko Harada ,  Hirotaka Uefuji ,  Ren Chen ,  Takeshi Bamba ,  Akio Kobayashi ,  Eiichiro Fukusaki ,  Kazumasa Hirata ,  Koichiro Gyokusen

IPC分类号： C12N15/82 ,  C12N15/11 ,  C12N9/10 ,  C12N15/00 ,  A01H5/00

CPC分类号： C12N15/8243 ,  C12N9/1085

摘要： A method for increasing the amount of trans-1,4-polyisoprene contained in a plant, and a method for effectively producing trans-1,4-polyisoprene using a plant are provided. A long-chain trans-prenyl diphosphate synthase gene that comprises DNA having at least one base sequence selected from the group consisting of a base sequence from positions 88 to 1134 of the base sequence of SEQ ID NO: 1 or a complementary sequence thereof, a base sequence from positions 42 to 1088 of the base sequence of SEQ D NO: 3 or a complementary sequence thereof, and a base sequence from positions 91 to 1140 of the base sequence of SEQ ID NO: 5 or a complementary sequence thereof are disclosed; as well as a plant transformed with an expression vector containing the gene.

摘要翻译： 提供了增加植物中所含的反式-1,4-聚异戊二烯的量的方法以及使用植物有效生产反式-1,4-聚异戊二烯的方法。 一种长链反式异戊二烯基二磷酸合成酶基因，其包含具有选自SEQ ID NO：1的碱基序列的第88至1134位的碱基序列或其互补序列的至少一种碱基序列的DNA， 公开了SEQ ID NO：3的碱基序列的位置42至1088的碱基序列或其互补序列，以及SEQ ID NO：5的碱基序列的第91至1140位的碱基序列或其互补序列; 以及用含有该基因的表达载体转化的植物。

公开(公告)号：US09127286B2

公开(公告)日：2015-09-08

申请号：US12550863

申请日：2009-08-31

申请人： Yoshihisa Nakazawa ,  Yoko Harada ,  Hirotaka Uefuji ,  Ren Chen ,  Takeshi Bamba ,  Akio Kobayashi ,  Eiichiro Fukusaki ,  Kazumasa Hirata ,  Koichiro Gyokusen

发明人： Yoshihisa Nakazawa ,  Yoko Harada ,  Hirotaka Uefuji ,  Ren Chen ,  Takeshi Bamba ,  Akio Kobayashi ,  Eiichiro Fukusaki ,  Kazumasa Hirata ,  Koichiro Gyokusen

IPC分类号： C12N15/82 ,  C12N9/10

CPC分类号： C12N15/8243 ,  C12N9/1085

摘要： A method for increasing the amount of trans-1,4-polyisoprene contained in a plant, and a method for effectively producing trans-1,4-polyisoprene using a plant are provided. A long-chain trans-prenyl diphosphate synthase gene that comprises DNA having at least one base sequence selected from the group consisting of a base sequence from positions 88 to 1134 of the base sequence of SEQ ID NO: 1 or a complementary sequence thereof, a base sequence from positions 42 to 1088 of the base sequence of SEQ ID NO: 3 or a complementary sequence thereof, and a base sequence from positions 91 to 1140 of the base sequence of SEQ ID NO: 5 or a complementary sequence thereof are disclosed; as well as a plant transformed with an expression vector containing the gene.

摘要翻译： 提供了增加植物中所含的反式-1,4-聚异戊二烯的量的方法以及使用植物有效生产反式-1,4-聚异戊二烯的方法。 一种长链反式异戊二烯基二磷酸合成酶基因，其包含具有选自SEQ ID NO：1的碱基序列的第88至1134位的碱基序列或其互补序列的至少一种碱基序列的DNA， 公开了SEQ ID NO：3的碱基序列的位置42至1088的碱基序列或其互补序列，以及SEQ ID NO：5的碱基序列的第91至1140位的碱基序列或其互补序列; 以及用含有该基因的表达载体转化的植物。