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公开(公告)号：US20060205006A1
公开(公告)日：2006-09-14
申请号：US11433722
申请日：2006-05-12
申请人： Tony Godfrey , James Luketich , Siva Raja , Lori Kelly , Sydney Finkelstein
发明人： Tony Godfrey , James Luketich , Siva Raja , Lori Kelly , Sydney Finkelstein
CPC分类号： C12Q1/6851 , C12Q1/6886 , C12Q2600/158 , C12Q2600/16 , C12Q2600/166 , C12Q2537/143 , C12Q2527/107 , C12Q2561/101
摘要： A method for balancing multiplexed PCR methods is provided. In the method, two or more sequential temporal PCR stages are used to effectively separate two or more PCR reactions in a single tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon by a first primer set and a second amplicon by a second primer set during the first and second amplification stages. Also provided are rapid RT-PCR methods that find particular use in intraoperative diagnoses and prognoses, for instance in diagnosing malignant esophageal adenocarcenoma by determining expression levels of carcinoembryonic antigen (CEA) in sentinel lymph nodes.
摘要翻译： 提供了一种平衡复用PCR方法的方法。 在该方法中，使用两个或多个连续的时间PCR阶段来有效地分离单个管中的两个或更多个PCR反应，作为引物限制的替代，以调节第一引物组和第二引物组的相对生产速率 在第一和第二扩增阶段期间由第二引物组扩增。 还提供了快速RT-PCR方法，其特别用于术中诊断和预后，例如通过确定前哨淋巴结中癌胚抗原（CEA）的表达水平来诊断恶性食管腺癌。
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2.发明申请Dynamic genomic deletion expansion and formulation of molecular marker panels for integrated molecular pathology diagnosis and characterization of tissue, cellular fluid, and pure fluid specimens 审中-公开动态基因组缺失扩增和分子标记物的制备用于组织，细胞液和纯液体标本的综合分子病理学诊断和表征公开(公告)号：US20060088871A1
公开(公告)日：2006-04-27
申请号：US11256152
申请日：2005-10-24
CPC分类号： G01N33/57438 , C12Q1/6806 , C12Q1/6827 , C12Q1/6886 , C12Q2600/106 , C12Q2600/112 , C12Q2600/118 , C12Q2600/154 , C12Q2600/156 , C12Q2545/114 , C12Q2527/125
摘要： Provided are materials, methods, platforms, and kits for diagnosing, prognosing, and/or determining the biological aggressiveness of a tumor (malignant and non-malignant) based in part on the temporal profile of genomic deletion expansion of the tumor. Also provided are methods of determining marker panels for different diseases and/or tissues and markers identified by these methods.
摘要翻译： 提供了部分基于肿瘤的基因组缺失扩增的时间特征的用于诊断，预测和/或确定肿瘤（恶性和非恶性）的生物攻击性的材料，方法，平台和试剂盒。 还提供了确定通过这些方法鉴定的不同疾病和/或组织和标志物的标记物组的方法。
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3.发明申请Molecular analysis of cellular fluid and liquid cytology specimens for clinical diagnosis, characterization, and integration with microscopic pathology evaluation 审中-公开细胞液和液体细胞学标本的分子分析用于临床诊断，表征和与微观病理学评估的整合公开(公告)号：US20060141497A1
公开(公告)日：2006-06-29
申请号：US11256150
申请日：2005-10-24
IPC分类号： C12Q1/68
CPC分类号： G01N33/57438 , C12Q1/6806 , C12Q1/6827 , C12Q1/6886 , C12Q2600/106 , C12Q2600/112 , C12Q2600/118 , C12Q2600/154 , C12Q2600/156 , C12Q2545/114 , C12Q2527/125
摘要： The application relates to methods, materials, kits, and devices for characterizing fluid specimens of any type or source that may or may not contain cells. The methods, materials, kits, and devices can be used for analyzing fluid obtained from various organs or near a purported tumor site, such as via breast ductal lavage or aspiration of a pancreatic cyst. The methods, materials, kits, and devices generate molecular information that can be used in conjunction with clinical, imaging, and microscopic pathology evaluation to provide a superior pathology diagnosis for clinical and research.
摘要翻译： 本申请涉及用于表征可能含有或可能不含有细胞的任何类型或来源的流体标本的方法，材料，试剂盒和装置。 方法，材料，试剂盒和装置可用于分析从各种器官或附近的所称肿瘤部位获得的流体，例如通过乳管导管灌洗或胰腺囊肿抽吸。 方法，材料，试剂盒和装置产生可与临床，成像和微观病理学评估结合使用的分子信息，为临床和研究提供优越的病理诊断。
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公开(公告)号：US20060154274A1
公开(公告)日：2006-07-13
申请号：US11289624
申请日：2005-11-30
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/6806 , C12Q1/6886 , C12Q2600/156
摘要： The present invention pertains to a method for topographic genotyping. The method comprises the steps of placing a biological specimen having DNA of a patient under a microscope. Then there is the step of inspecting the biological specimen microscopically with the microscope. Next there is the step of choosing a microscope size target on the biological specimen based on its histopathologic characteristics. Next there is the step of separating the target from the specimen. Then there is the step of obtaining DNA sequences from the target so the DNA sequences can be amplified. Next there is the step of amplifying the DNA sequences. Then there is the step of detecting mutations in the DNA sequences. The present invention pertains to a method for topographic genotyping. The method comprises the steps of separating a section from a specimen of fixative treated tissue. Then there is the step of obtaining DNA sequences from the section. Next there is the step of amplifying the DNA sequences by cyphaling them in a PCR machine, with each cycle heating them to a temperature no greater than 99° C., and then back to a temperature of 55° C. in 5 minutes. Next there is the step of detecting mutations in the DNA sequences. Preferably, the separating step includes the step of cutting one to three 2-6 micron thick histeologic sections from the specimen.
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5.发明申请Enhanced amplifiability of minute fixative-treated tissue samples, minute stained cytology samples, and other minute sources of DNA 审中-公开分子固定治疗组织样本，细胞染色细胞学样本和其他细小的DNA来源的增强的可扩增性公开(公告)号：US20060115844A1
公开(公告)日：2006-06-01
申请号：US11255980
申请日：2005-10-24
CPC分类号： G01N33/57438 , C12Q1/6806 , C12Q1/6827 , C12Q1/6886 , C12Q2600/106 , C12Q2600/112 , C12Q2600/118 , C12Q2600/154 , C12Q2600/156 , C12Q2545/114 , C12Q2527/125
摘要： The specification provides materials and methods for enhancing the amplifiability of nucleic acids that are present in minute amounts. Preferably, the materials and methods are utilized in combination with pathology techniques to amplify nucleic acids obtained from fixative and/or stained tissue or cell samples. The amplified nucleic acids can then be utilized for rendering a diagnosis or prognosis to the subject from which the tissue or cellular sample was obtained.
摘要翻译： 本说明书提供用于提高以微量存在的核酸的可扩增性的材料和方法。 优选地，将材料和方法与病理技术结合使用以扩增从固定和/或染色的组织或细胞样品获得的核酸。 然后可以将扩增的核酸用于对获得组织或细胞样品的受试者进行诊断或预后。
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6.发明申请Topographic genotyping for determining the diagnosis, malignant potential, and biologic behavior of pancreatic cysts and related conditions 审中-公开用于确定胰腺囊肿的诊断，恶性潜力和生物学行为的地形基因分型及相关条件公开(公告)号：US20060088870A1
公开(公告)日：2006-04-27
申请号：US11255978
申请日：2005-10-24
CPC分类号： G01N33/57438 , C12Q1/6806 , C12Q1/6827 , C12Q1/6886 , C12Q2600/106 , C12Q2600/112 , C12Q2600/118 , C12Q2600/154 , C12Q2600/156 , C12Q2545/114 , C12Q2527/125
摘要： The application relates to a method of a predicting the presence of invasive pancreatic cancer or high grade dysplasia, pre-cancerous pancreatic states and non-neoplastic conditions comprising detailed molecular analysis incorporating DNA quality and quantity, K-ras mutational analysis and a broad spectrum of tumor suppressor gene linked microsatellite LOH. Methods of diagnosing, determining prognosis of and determining a course of treatment for pancreatic cancer or high grade dysplasia, pre-cancerous pancreatic states and non-neoplastic conditions are also provided.
摘要翻译： 该应用涉及一种预测侵袭性胰腺癌或高级别不典型增生，癌前胰腺状态和非肿瘤病变的存在的方法，其包括结合DNA质量和数量的详细分子分析，K-ras突变分析和广谱 肿瘤抑制基因连锁微卫星LOH。 还提供了诊断，确定胰腺癌或高级别发育异常，癌前胰腺状态和非肿瘤状况的预后和确定治疗过程的方法。
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