公开(公告)号：US06906859B2

公开(公告)日：2005-06-14

申请号：US10453660

申请日：2003-06-04

申请人： Toshiaki Nihoshi ,  Hisashi Okugawa

发明人： Toshiaki Nihoshi ,  Hisashi Okugawa

IPC分类号： G02B21/06 ,  G02B21/08 ,  G02B21/16

CPC分类号： G02B21/08 ,  G02B21/06 ,  G02B21/16

摘要： An epi-illumination apparatus for fluorescent observation, adjusting light intensities of a plurality of illuminations on a sample over a wide wavelength band continuously and being configured inexpensively, and a fluorescence microscope having the same are provided. A light source, extracting means, an aperture stop, and a filter are arranged on a predetermined optical axis. The aperture stop is arranged on a plane generally conjugated with a pupil plane of an objective. The filter is placed near the aperture stop. The extracting means extracts a plurality of narrow wavelength bands from the wavelength band of the illumination emitted from the light source. The filter has regions of different spectral transmission characteristics to the narrow wavelength bands. The adjusting means for adjusting light intensities of transmitted light from the filter in the narrow wavelength bands independently by moving the filter in a direction orthogonal to the optical axis is provided.

摘要翻译： 一种用于荧光观察的外照明装置，连续地调节宽波长带上的样品上的多个照明的光强度并且被廉价地配置，并且提供具有该照明装置的荧光显微镜。 在预定的光轴上布置有光源，提取装置，孔径光阑和滤光器。 孔径光阑布置在通常与物镜的光瞳平面共轭的平面上。 过滤器放置在孔径光阑附近。 提取装置从从光源发射的照明的波长带中提取多个窄波长带。 滤波器具有与窄波长带不同的频谱传输特性的区域。 提供了一种调节装置，用于通过沿与光轴正交的方向移动滤光器来独立地调节来自窄波长带中的滤光器的透射光的光强度。

公开(公告)号：US20090201366A1

公开(公告)日：2009-08-13

申请号：US12429181

申请日：2009-04-23

申请人： Ichiro Sase ,  Toshiaki Nihoshi

发明人： Ichiro Sase ,  Toshiaki Nihoshi

IPC分类号： H04N7/18

CPC分类号： G02B21/361 ,  G03B17/48

摘要： Providing an imaging apparatus and a microscope capable of taking two-dimensional images of a sample at a plurality of observation positions different in the optical axis direction at the same time. The apparatus includes an image-forming lens 15 that forms images of a sample 4 on a plurality of image-forming places; an optical-path-dividing member 17, 18, 19 that divides an optical path from the same area in a plane perpendicular to an optical axis of the sample 4 so as to form the plurality of image-forming places; and an optical-path-length-changing member 27, 28, 29 that is provided on at least one optical path between the plurality of image-forming places and the imaging lens 15.

摘要翻译： 提供能够同时在光轴方向上的多个观察位置拍摄样品的二维图像的成像装置和显微镜。 该装置包括在多个成像位置形成样品4的图像的图像形成透镜15; 在垂直于样品4的光轴的平面中从相同的区域分割光路以形成多个图像形成位置的光路分割部件17,18,19; 以及设置在多个图像形成处与成像透镜15之间的至少一个光路上的光路长度变化部件27,28,29。

公开(公告)号：US06128128A

公开(公告)日：2000-10-03

申请号：US854465

申请日：1997-05-12

申请人： Tatsuro Otaki ,  Toshiaki Nihoshi ,  Manabu Sato ,  Hitoshi Kaizu ,  Yumiko Ouchi

发明人： Tatsuro Otaki ,  Toshiaki Nihoshi ,  Manabu Sato ,  Hitoshi Kaizu ,  Yumiko Ouchi

IPC分类号： G02B21/24 ,  G02B21/02 ,  G02B21/00

CPC分类号： G02B21/02

摘要： A microscope system for observing an image of a sample at desired magnifications includes a first objective lens having an objective side surface which is telecentric, a focusing lens, disposed in an optical path along which optical rays emitted from the first objective lens travel, for focusing the optical rays and forming a sample image and a second objective lens having a magnification factor different from that of the first objective lens. An interchanging member holds the first objective lens and the second objective lens and is used for placing one of the first and second objective lenses in an observational optical path between the sample and the focusing lens. The inequality 0.29

摘要翻译： 用于以期望的倍率观察样品的图像的显微镜系统包括具有远心的物镜侧表面的第一物镜，设置在从第一物镜行进的光线穿过的光路中的聚焦透镜，用于聚焦 并且形成样本图像和具有与第一物镜不同的放大倍率的第二物镜。 交换构件保持第一物镜和第二物镜，并用于将第一和第二物镜之一放置在样品和聚焦透镜之间的观察光路中。 假设D为相当于样品的表面与物镜的附着面之间的距离的ff的焦距，fI为聚焦透镜的焦距，则满足不等式0.29

公开(公告)号：US4527869A

公开(公告)日：1985-07-09

申请号：US499549

申请日：1983-05-31

申请人： Toshiaki Nihoshi

发明人： Toshiaki Nihoshi

IPC分类号： G02B21/00 ,  G02B21/18 ,  G02B21/36

CPC分类号： G02B21/361 ,  G02B21/18

摘要： A microscope provided with a photographing device comprises an objective lens, prism means having a first surface opposed to the objective lens, a second surface parallel to the first surface, and a third and a fourth surface inclined by a predetermined angle with respect to the first and second surfaces and parallel to each other, the prism means introducing thereinto the emergent light from the objective lens through said first surface and causing the light to emerge from said second surface, light guide means for causing the emergent light from the second surface of the prism means to enter the third surface thereof, change-over means operated to change an optical path determined by the light guide means and direct the emergent light from said second surface to the photographing device, and an eyepiece system for receiving the emergent light from the fourth surface of the prism means.

摘要翻译： 具有拍摄装置的显微镜包括物镜，具有与物镜相对的第一表面的棱镜装置，与第一表面平行的第二表面，以及相对于第一透镜倾斜预定角度的第三和第四表面 和第二表面并且彼此平行，所述棱镜装置通过所述第一表面将来自所述物镜的出射光引入所述第一表面，并使所述光从所述第二表面出射;导光装置，用于使来自所述第一表面的所述第二表面的出射光 棱镜装置进入其第三表面，转换装置被操作以改变由导光装置确定的光路并将出射光从所述第二表面引导到拍摄装置，以及目镜系统，用于接收来自 棱镜装置的第四表面。

公开(公告)号：US08098279B2

公开(公告)日：2012-01-17

申请号：US12429181

申请日：2009-04-23

申请人： Ichiro Sase ,  Toshiaki Nihoshi

发明人： Ichiro Sase ,  Toshiaki Nihoshi

IPC分类号： H04N7/18

CPC分类号： G02B21/361 ,  G03B17/48

摘要： Providing an imaging apparatus and a microscope capable of taking two-dimensional images of a sample at a plurality of observation positions different in the optical axis direction at the same time. The apparatus includes an image-forming lens 15 that forms images of a sample 4 on a plurality of image-forming places; an optical-path-dividing member 17, 18, 19 that divides an optical path from the same area in a plane perpendicular to an optical axis of the sample 4 so as to form the plurality of image-forming places; and an optical-path-length-changing member 27, 28, 29 that is provided on at least one optical path between the plurality of image-forming places and the imaging lens 15.

摘要翻译： 提供能够同时在光轴方向上的多个观察位置拍摄样品的二维图像的成像装置和显微镜。 该装置包括在多个成像位置形成样品4的图像的图像形成透镜15; 在垂直于样品4的光轴的平面中从相同的区域分割光路以形成多个图像形成位置的光路分割部件17,18,19; 以及设置在多个图像形成处与成像透镜15之间的至少一个光路上的光路长度变化部件27,28,29。

公开(公告)号：US20100014158A1

公开(公告)日：2010-01-21

申请号：US12563141

申请日：2009-09-20

申请人： Toshiaki Nihoshi

发明人： Toshiaki Nihoshi

IPC分类号： G02B21/06

CPC分类号： G02B21/0076 ,  G01N21/6458 ,  G02B21/008

摘要： A microscope apparatus 1 comprises: an illumination optical system illuminating a sample with laser light from laser light sources 32, 42; a fluorescence detection optical system that detects fluorescence from the sample; a plurality of fluorescence cubes 61 that are provided on an optical path of the illumination optical system and leads the laser light to the sample; and an objective lens 16. At least one of the fluorescence cube includes an optical member 60 that makes a principal ray of the laser light substantially parallel to an optical axis of the illumination optical system and concentrates the laser light on a given position that is on a pupil position P of the objective lens and separates from the optical axis, thereby providing a microscope apparatus capable of changing from a confocal microscope to a total-internal-reflection fluorescence microscope by exchanging a fluorescence cube used in the fluorescence microscope.

摘要翻译： 显微镜装置1包括：照明光学系统，用来自激光光源32,42的激光照射样品; 检测来自样品的荧光的荧光检测光学系统; 设置在照明光学系统的光路上并将激光导向样品的多个荧光立方体61; 和物镜16.荧光立方体中的至少一个包括光学构件60，其使激光的主光线基本上平行于照明光学系统的光轴并将激光聚集在给定的位置上 物镜的光瞳位置P并与光轴分离，从而提供能够通过交换荧光显微镜中使用的荧光立方体从共聚焦显微镜改变为全内反射荧光显微镜的显微镜装置。

公开(公告)号：US06963446B2

公开(公告)日：2005-11-08

申请号：US10315162

申请日：2002-12-10

申请人： Toshiaki Nihoshi

发明人： Toshiaki Nihoshi

IPC分类号： G02B21/06 ,  G02B21/08

CPC分类号： G02B21/082

摘要： An epi-illumination apparatus has, in the following order from an light source device side, a first imaging lens which forms an image of the light source and a field lens which projects the image of the light source to an object to be illuminated. Either one of a first magnification varying unit or a second magnification varying unit is selectively disposed between the first imaging lens and the field lens in order to vary a magnification of the image of the light source. The first magnification varying unit has a first aperture stop and a second imaging lens that is disposed in the field lens side of the first aperture stop, while the second magnification varying unit has a second aperture stop and a third imaging lens that is disposed in the first imaging lens side of the second aperture stop.

摘要翻译： 外照射装置从光源装置侧依次具有形成光源的图像的第一摄像镜头和将光源的图像投影到被照射物体的场透镜。 选择性地将第一变倍单位或第二变倍单位中的任一个选择性地设置在第一成像透镜和场透镜之间，以便改变光源的图像的放大率。 第一放大率变化单元具有设置在第一孔径光阑的场透镜侧中的第一孔径光阑和第二成像透镜，而第二倍率改变单元具有第二孔径光阑和第三成像透镜， 第二孔径光阑的第一成像透镜侧。

公开(公告)号：US4512640A

公开(公告)日：1985-04-23

申请号：US496402

申请日：1983-05-20

申请人： Toshiaki Nihoshi

发明人： Toshiaki Nihoshi

IPC分类号： G02B21/00 ,  G02B21/24 ,  G02B27/28 ,  G02B15/10 ,  G02B27/00

CPC分类号： G02B21/0004 ,  G02B21/24

摘要： A polarizing microscope comprises an orthoscopic system having an objective lens for forming the image of a sample, and a conoscopic system including polarizer means, analyzer means and an image forming lens disposed between the objective lens and the image plane of the image of the sample. The image forming lens is disposed at a position whereat the exit pupil of the objective lens and said image plane are conjugate with respect to the image forming lens and has a sufficiently small diameter relative to the width of the optical path of the orthoscopic system in said position. Thus, the conoscopic system forms the image of the exit pupil of the objective lens on the image plane and the observation light beam by the orthoscopic system passes through the outside of the image forming lens and forms the image of the sample around the image of the exit pupil on said image plane.

摘要翻译： 偏振显微镜包括具有用于形成样本图像的物镜的无畸变系统，以及包括偏振器装置，分析器装置和设置在物镜和图像的图像平面之间的图像形成透镜的圆锥镜系统。 成像透镜设置在物镜的出射光瞳和所述像平面相对于图像形成透镜共轭的位置处，并且具有相对于所述无畸变系统的光路的宽度具有足够小的直径 位置。 因此，虹膜系统在像面上形成物镜的出射光瞳的图像，并且由无畸变系统观察的光束通过成像透镜的外部，并且形成围绕图像的图像的样本的图像 在所述图像平面上出射光瞳。

公开(公告)号：US08014065B2

公开(公告)日：2011-09-06

申请号：US12563141

申请日：2009-09-20

申请人： Toshiaki Nihoshi

发明人： Toshiaki Nihoshi

IPC分类号： G02B21/06

CPC分类号： G02B21/0076 ,  G01N21/6458 ,  G02B21/008

摘要： A microscope apparatus has an illumination optical system illuminating a sample with laser light from laser light sources. A fluorescence detection optical system detects fluorescence from the sample. Fluorescence cubes are interchangeably provided in an optical path of the illumination optical system and lead the laser light to the sample. An objective lens is also provided. At least one of the fluorescence cubes includes an optical member that makes a principal ray of the laser light substantially parallel to an optical axis of the illumination optical system and concentrates the laser light on a given position that is on a pupil position of the objective lens and separated from the optical axis, thereby providing a microscope apparatus capable of changing from a confocal microscope to a total-internal-reflection fluorescence microscope by exchanging a fluorescence cube used in the fluorescence microscope.

摘要翻译： 显微镜装置具有用来自激光光源的激光照射样品的照明光学系统。 荧光检测光学系统检测来自样品的荧光。 荧光立方体可互换地设置在照明光学系统的光路中，并将激光引导到样品。 还提供物镜。 荧光立方体中的至少一个包括使激光的主光线基本上平行于照明光学系统的光轴并将激光集中在位于物镜的光瞳位置的给定位置的光学部件 并且与光轴分离，从而提供一种能够通过交换荧光显微镜中使用的荧光立方体从共聚焦显微镜改变为全内反射荧光显微镜的显微镜装置。

公开(公告)号：US07071451B2

公开(公告)日：2006-07-04

申请号：US10461374

申请日：2003-06-16

申请人： Aiichi Ishikawa ,  Toshiaki Nihoshi

发明人： Aiichi Ishikawa ,  Toshiaki Nihoshi

IPC分类号： G02B27/40

CPC分类号： G02B21/245

摘要： An autofocus system according to the present invention comprises: a light source; a focusing illumination optical system that forms an optical image generated with light from the light source on a target object through an objective lens; a focusing image forming optical system that receives through the objective lens reflected light generated as the optical image is reflected off the target object and forms a reflected image of the optical image; a photoelectric converter that is provided at an image forming position at which the reflected image is formed by the focusing image forming optical system to detect the reflected image; a signal output device that outputs a signal for controlling a focus actuator based upon a signal corresponding to the reflected image obtained at the photoelectric converter; and an image forming position adjustment device that adjusts an offset quantity between a focus position of the objective lens and an image forming position of the optical image by moving at least one of the image forming position of the optical image and the image forming position of the reflected image along an optical axis.

摘要翻译： 根据本发明的自动对焦系统包括：光源; 聚焦照明光学系统，其通过物镜形成由来自光源的目标物体的光产生的光学图像; 通过物镜接收的聚焦图像形成光学系统作为光学图像产生的反射光从目标物体反射并形成光学图像的反射图像; 光电转换器，设置在图像形成位置处，由所述聚焦图像形成光学系统形成所述反射图像以检测所述反射图像; 信号输出装置，其基于与在所述光电转换器处获得的反射图像相对应的信号输出用于控制聚焦致动器的信号; 以及图像形成位置调整装置，其通过移动所述光学图像的图像形成位置和所述图像形成位置中的至少一个来调整所述物镜的焦点位置与所述光学图像的图像形成位置之间的偏移量 沿着光轴的反射图像。