公开(公告)号：US6143533A

公开(公告)日：2000-11-07

申请号：US525323

申请日：2000-03-13

申请人： Toshihisa Sumi ,  Hideki Ohba ,  Toru Ikegami ,  Masao Shibata ,  Tsuyoshi Sakaki ,  Imre Sallay ,  Sung Soo Park

发明人： Toshihisa Sumi ,  Hideki Ohba ,  Toru Ikegami ,  Masao Shibata ,  Tsuyoshi Sakaki ,  Imre Sallay ,  Sung Soo Park

IPC分类号： C07H7/027 ,  A61K35/56 ,  C07H1/08 ,  C12P7/58 ,  C12P7/40 ,  A61K35/12

CPC分类号： C12P7/58

摘要： Disclosed is an efficient and economical method for the preparation of N-glycolyl neuraminic acid in a high purity from an inexpensive abundant source material. The method comprises dispersing body tissues of an echinodermatous marine animal Cucumaria echinata in an aqueous medium, preferably, using a dry powder of the tissues prepared in advance, in which the tissues are proteolytically decomposed to isolate N-glycolyl neuraminic acid in the form of an aqueous solution containing polypeptides as a by-product, followed by separation of N-glycolyl neuraminic acid from the aqueous solution by removing the polypeptides and purification of the compound in a process utilizing an ion-exchange treatments.

摘要翻译： 公开了一种从廉价丰富的原料制备高纯度的N-羟乙酰神经氨酸的有效和经济的方法。 该方法包括将棘皮动物海洋动物黄瓜（Cucumaria echinata）的身体组织分散在水性介质中，优选使用预先制备的组织的干粉末，其中组织被蛋白水解分解以分离出N-羟乙酰神经氨酸 水溶液，其含有作为副产物的多肽，随后通过在使用离子交换处理的方法中除去多肽和化合物的纯化从水溶液中分离N-羟乙酰神经氨酸。

公开(公告)号：US06342367B1

公开(公告)日：2002-01-29

申请号：US09513191

申请日：2000-02-25

申请人： Toshihisa Sumi ,  Hideki Ohba ,  Toru Ikegami ,  Masao Shibata ,  Tsuyoshi Sakaki ,  Imre Sallay ,  Sung Soo Park

发明人： Toshihisa Sumi ,  Hideki Ohba ,  Toru Ikegami ,  Masao Shibata ,  Tsuyoshi Sakaki ,  Imre Sallay ,  Sung Soo Park

IPC分类号： C12P2106

CPC分类号： C12P19/26

摘要： Disclosed is an economical method for the preparation of chondroitin sulfates A and C useful as an effective ingredient of medicaments from fish scales as a waste material discharged from fishery in large quantities. Fish scales are enzymatically decomposed in an aqueous medium in the presence of a protease to isolate the chondroitin sulfate compounds and by-product polypeptides followed by removal of the by-product polypeptides from the aqueous solution by a cation-exchange treatment and then the aqueous solution of the chondroitin sulfate compounds is subjected to fractional precipitation by the addition of ethyl alcohol as the precipitant.

摘要翻译： 公开了一种用于制备硫酸软骨素A和C的经济方法，其用作作为大量从渔业排放的废物的鱼鳞的药物的有效成分。 鱼鳞在蛋白酶存在下在水性介质中酶促分解，分离出硫酸软骨素化合物和副产物多肽，然后通过阳离子交换处理从水溶液中除去副产物多肽，然后将水溶液 的硫酸软骨素化合物通过加入乙醇作为沉淀剂进行分级沉淀。