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公开(公告)号：US20090155760A1
公开(公告)日：2009-06-18
申请号：US10562833
申请日：2004-07-02
IPC分类号： A01N1/02
摘要： A method of injecting cells into a biological tissue comprising thrusting injection needle (200) under microvibration into tissue (5) secured by suction to tissue suction means (210) and effecting injection of a cell suspension into the tissue through the needle. There is also provided an apparatus for cell injection into the biological tissue.
摘要翻译： 将细胞注入生物组织中的方法，所述生物组织包括在微振动下的推进注射针（200）进入组织（5），所述组织通过抽吸固定到组织抽吸装置（210），并通过所述针实现细胞悬浮液注入所述组织。 还提供了用于细胞注入生物组织的装置。
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公开(公告)号：US20060115900A1
公开(公告)日：2006-06-01
申请号：US10538716
申请日：2003-12-11
IPC分类号： C12N5/00
CPC分类号： A61L27/3691 , A61L27/3604 , A61L27/3683 , A61L27/3687 , A61L2430/40
摘要： A method of efficiently removing or fixing donor cells from a native tissue of mammalian origin comprises immersing the tissue in a treating solution, and irradiating the tissue with microwave while maintaining the temperature thereof in the range between 0° C. and 40° C.
摘要翻译： 从哺乳动物来源的天然组织有效地去除或固定供体细胞的方法包括将组织浸入处理溶液中，并用微波照射组织，同时将其温度保持在0℃至40℃的范围内。
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3.发明申请Device and method for uniformly inoculating a substrate having three-dimensional surfaces 有权用于均匀接种具有三维表面的基材的装置和方法公开(公告)号：US20070166818A1
公开(公告)日：2007-07-19
申请号：US10568701
申请日：2004-08-18
申请人： Toshiya Fujisato , Takuro Kogoshi , Yusuke Suga , Akio Kishida , Takeshi Nakatani , Soichiro Kitamura
发明人： Toshiya Fujisato , Takuro Kogoshi , Yusuke Suga , Akio Kishida , Takeshi Nakatani , Soichiro Kitamura
CPC分类号： C12M27/10
摘要： An apparatus and method for inoculating a biological substrate with cells include provision for rotating a container in which the substrate and a cell suspension are received about first and second axes of rotation. The apparatus comprises said container, first drive means for rotatably supporting the container about the first axis, and second drive means for rotatably supporting the first drive means about the second axis. The first and second axes of rotation do not lie in the same plane and extend in different directions so that the container revolves around the second axis while the container is rotating about the first axis. The inoculation method utilizes this apparatus.
摘要翻译： 用于用细胞接种生物底物的装置和方法包括提供用于旋转其中容纳基质和细胞悬浮液的容器绕第一和第二旋转轴旋转的装置。 该装置包括所述容器，用于围绕第一轴线可旋转地支撑容器的第一驱动装置和用于围绕第二轴线可旋转地支撑第一驱动装置的第二驱动装置。 第一和第二旋转轴线不位于相同的平面中并且在不同方向上延伸，使得当容器围绕第一轴线旋转时，容器围绕第二轴线旋转。 接种方法利用该装置。
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4.发明授权Device and method for uniformly inoculating a substrate having three-dimensional surfaces 有权用于均匀接种具有三维表面的基材的装置和方法公开(公告)号：US07910360B2
公开(公告)日：2011-03-22
申请号：US10568701
申请日：2004-08-18
申请人： Toshiya Fujisato , Takuro Kogoshi , Yusuke Suga , Akio Kishida , Takeshi Nakatani , Soichiro Kitamura
发明人： Toshiya Fujisato , Takuro Kogoshi , Yusuke Suga , Akio Kishida , Takeshi Nakatani , Soichiro Kitamura
CPC分类号： C12M27/10
摘要： An apparatus and method for inoculating a biological substrate with cells include provision for rotating a container in which the substrate and a cell suspension are received about first and second axes of rotation. The apparatus comprises said container, first drive means for rotatably supporting the container about the first axis, and second drive means for rotatably supporting the first drive means about the second axis. The first and second axes of rotation do not lie in the same plane and extend in different directions so that the container revolves around the second axis while the container is rotating about the first axis. The inoculation method utilizes this apparatus.
摘要翻译： 用于用细胞接种生物底物的装置和方法包括提供用于旋转其中容纳基质和细胞悬浮液的容器绕第一和第二旋转轴旋转的装置。 该装置包括所述容器，用于围绕第一轴线可旋转地支撑容器的第一驱动装置和用于围绕第二轴线可旋转地支撑第一驱动装置的第二驱动装置。 第一和第二旋转轴线不位于相同的平面中并且在不同方向上延伸，使得当容器围绕第一轴线旋转时，容器围绕第二轴线旋转。 接种方法利用该装置。
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公开(公告)号：US08323881B2
公开(公告)日：2012-12-04
申请号：US10562833
申请日：2004-07-02
摘要： A method of injecting cells into a biological tissue comprising thrusting injection needle (200) under microvibration into tissue (5) secured by suction to tissue suction means (210) and effecting injection of a cell suspension into the tissue through the needle. There is also provided an apparatus for cell injection into the biological tissue.
摘要翻译： 将细胞注入生物组织中的方法，所述生物组织包括在微振动下的推进注射针（200）进入组织（5），所述组织通过抽吸固定到组织抽吸装置（210），并通过所述针实现细胞悬浮液注入所述组织。 还提供了用于细胞注入生物组织的装置。
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公开(公告)号：US07883864B2
公开(公告)日：2011-02-08
申请号：US10538716
申请日：2003-12-11
CPC分类号： A61L27/3691 , A61L27/3604 , A61L27/3683 , A61L27/3687 , A61L2430/40
摘要： A method of efficiently removing or fixing donor cells from a native tissue of mammalian origin comprises immersing the tissue in a treating solution, and irradiating the tissue with microwave while maintaining the temperature thereof in the range between 0° C. and 40° C.
摘要翻译： 从哺乳动物来源的天然组织有效地去除或固定供体细胞的方法包括将组织浸入处理溶液中，并用微波照射组织，同时将其温度保持在0℃至40℃的范围内。
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7.发明申请Method of treating biological tissue for transplantation by applying ultrahigh hydrostatic pressure 审中-公开通过应用超高静水压力处理生物组织进行移植的方法公开(公告)号：US20060110720A1
公开(公告)日：2006-05-25
申请号：US10527312
申请日：2003-09-09
CPC分类号： A61L27/3691 , A61K35/34 , A61K35/44 , A61L27/3683 , A61L2430/40
摘要： Native tissue of mammalian origin are treated for transplantation by applying ultrahigh hydrostatic pressure to the tissue in a liquid medium. The connective tissue is rendered isolatable from the remainder of the tissue including cellular components while keeping intact the structure and biomechanical properties thereof.
摘要翻译： 哺乳动物来源的本地组织通过在液体培养基中对组织施加超高静水压力来处理移植。 结缔组织与组织的其余部分可分离，包括细胞组分，同时保持其结构和生物力学性质的完整性。
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