公开(公告)号：US20210180041A1

公开(公告)日：2021-06-17

申请号：US17130114

申请日：2020-12-22

Applicant: Translate Bio, Inc.

Inventor： Frank DeRosa ,  Michael Heartlein ,  Jonathan Abysalh ,  Daniel Crawford ,  Anusha Dias ,  Shrirang Karve

IPC: C12N15/10 ,  C07H21/04

Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, a step of centrifuging an mRNA suspension in a centrifuge comprising a porous substrate at a speed sufficient to remove process contaminants and to precipitate purified mRNA composition onto the porous substrate.

公开(公告)号：US20210123041A1

公开(公告)日：2021-04-29

申请号：US17070877

申请日：2020-10-14

Applicant: Translate Bio, Inc.

Inventor： Jonathan Abysalh ,  Daniel Crawford ,  Frank DeRosa ,  Shrirang Karve ,  Anusha Dias ,  Michael Heartlein

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6806

Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.

公开(公告)号：US20240018514A1

公开(公告)日：2024-01-18

申请号：US18065952

申请日：2022-12-14

Applicant: Translate Bio, Inc.

Inventor： Jonathan Abysalh ,  Daniel Crawford ,  Frank DeRosa ,  Anusha Dias ,  Michael Heartlein

IPC: C12N15/10 ,  C12P19/34 ,  C12N9/12 ,  G01N27/447

CPC classification number: C12N15/1096 ,  C12Y207/07006 ,  G01N27/44778 ,  C12N9/1247 ,  C12N15/1068 ,  C12P19/34

Abstract: The present invention provides methods for large-scale production of a composition enriched for full-length mRNA molecules using an SP6 RNA polymerase and compositions produced using such methods and uses thereof.

公开(公告)号：US10266843B2

公开(公告)日：2019-04-23

申请号：US15482431

申请日：2017-04-07

Applicant: Translate Bio, Inc.

Inventor： Frank DeRosa ,  Michael Heartlein ,  Daniel Crawford ,  Shrirang Karve

IPC: C07H21/02 ,  C07H21/04 ,  C12N15/85 ,  C07K14/505 ,  C12N9/10 ,  C12N9/02 ,  C07K14/705 ,  A61K48/00 ,  A61K38/17 ,  A61K38/44 ,  A61K38/45 ,  A61K38/18 ,  A61K31/7115 ,  A61K31/7125 ,  C12P21/00 ,  C12N15/67 ,  A01K67/00

Abstract: The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3′ ends such that the multimeric coding nucleic acid compound comprises two or more 5′ ends.

公开(公告)号：US10975369B2

公开(公告)日：2021-04-13

申请号：US15907086

申请日：2018-02-27

Applicant: Translate Bio, Inc.

Inventor： Frank DeRosa ,  Michael Heartlein ,  Jonathan Abysalh ,  Daniel Crawford ,  Anusha Dias ,  Shrirang Karve

IPC: C12Q1/68 ,  C07H21/00 ,  C12N15/10 ,  C07H21/04

Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, a step of centrifuging an mRNA suspension in a centrifuge comprising a porous substrate at a speed sufficient to remove process contaminants and to precipitate purified mRNA composition onto the porous substrate.

公开(公告)号：US11976272B2

公开(公告)日：2024-05-07

申请号：US17070877

申请日：2020-10-14

Applicant: Translate Bio, Inc.

Inventor： Jonathan Abysalh ,  Daniel Crawford ,  Frank DeRosa ,  Shrirang Karve ,  Anusha Dias ,  Michael Heartlein

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6806

CPC classification number: C12N15/1017 ,  B01L3/502753 ,  C12Q1/6806 ,  B01L2200/028 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2400/0409 ,  C12Q2523/113 ,  C12Q2523/308 ,  C12Y201/03003

Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.

公开(公告)号：US11560562B2

公开(公告)日：2023-01-24

申请号：US15907163

申请日：2018-02-27

Applicant: Translate Bio, Inc.

Inventor： Anusha Dias ,  Daniel Crawford ,  Frank DeRosa ,  Jonathan Abysalh ,  Michael Heartlein

IPC: C12N15/10 ,  C12P19/34 ,  C12N9/12 ,  G01N27/447

Abstract: The present invention provides methods for large-scale production of a composition enriched for full-length mRNA molecules using an SP6 RNA polymerase and compositions produced using such methods and uses thereof.

公开(公告)号：US20220073944A1

公开(公告)日：2022-03-10

申请号：US17404283

申请日：2021-08-17

Applicant: TRANSLATE BIO, INC.

Inventor： Frank DeRosa ,  Michael Heartlein ,  Daniel Crawford ,  Shrirang Karve

IPC: C12N15/85 ,  C07K14/505 ,  C12N9/10 ,  C12N9/02 ,  C07K14/705 ,  A61K48/00 ,  A61K38/17 ,  A61K38/44 ,  A61K38/45 ,  A61K38/18 ,  A61K31/7115 ,  A61K31/7125 ,  C07H21/02 ,  C12N15/67 ,  C12P21/00

Abstract: The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3′ ends such that the multimeric coding nucleic acid compound comprises two or more 5′ ends.

公开(公告)号：US20200172923A1

公开(公告)日：2020-06-04

申请号：US16540791

申请日：2019-08-14

Applicant: Translate Bio, Inc.

Inventor： Frank DeRosa ,  Michael Heartlein ,  Daniel Crawford ,  Shrirang Karve

IPC: C12N15/85 ,  C12P21/00 ,  C12N15/67 ,  C07H21/02 ,  A61K31/7125 ,  A61K31/7115 ,  A61K38/18 ,  A61K38/45 ,  A61K38/44 ,  A61K38/17 ,  A61K48/00 ,  C07K14/705 ,  C12N9/02 ,  C12N9/10 ,  C07K14/505

Abstract: The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3′ ends such that the multimeric coding nucleic acid compound comprises two or more 5′ ends.

公开(公告)号：US11124804B2

公开(公告)日：2021-09-21

申请号：US16540791

申请日：2019-08-14

Applicant: Translate Bio, Inc.

Inventor： Frank DeRosa ,  Michael Heartlein ,  Daniel Crawford ,  Shrirang Karve

IPC: C07H21/02 ,  A61K31/7115 ,  A61K31/7125 ,  C12N15/85 ,  C07K14/505 ,  C12N9/10 ,  C12N9/02 ,  C07K14/705 ,  A61K48/00 ,  A61K38/17 ,  A61K38/44 ,  A61K38/45 ,  A61K38/18 ,  C12N15/67 ,  C12P21/00 ,  A01K67/00

Abstract: The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3′ ends such that the multimeric coding nucleic acid compound comprises two or more 5′ ends.