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公开(公告)号:US20210180041A1
公开(公告)日:2021-06-17
申请号:US17130114
申请日:2020-12-22
Applicant: Translate Bio, Inc.
Inventor: Frank DeRosa , Michael Heartlein , Jonathan Abysalh , Daniel Crawford , Anusha Dias , Shrirang Karve
Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, a step of centrifuging an mRNA suspension in a centrifuge comprising a porous substrate at a speed sufficient to remove process contaminants and to precipitate purified mRNA composition onto the porous substrate.
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公开(公告)号:US20210123041A1
公开(公告)日:2021-04-29
申请号:US17070877
申请日:2020-10-14
Applicant: Translate Bio, Inc.
Inventor: Jonathan Abysalh , Daniel Crawford , Frank DeRosa , Shrirang Karve , Anusha Dias , Michael Heartlein
IPC: C12N15/10 , B01L3/00 , C12Q1/6806
Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.
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公开(公告)号:US20240018514A1
公开(公告)日:2024-01-18
申请号:US18065952
申请日:2022-12-14
Applicant: Translate Bio, Inc.
Inventor: Jonathan Abysalh , Daniel Crawford , Frank DeRosa , Anusha Dias , Michael Heartlein
IPC: C12N15/10 , C12P19/34 , C12N9/12 , G01N27/447
CPC classification number: C12N15/1096 , C12Y207/07006 , G01N27/44778 , C12N9/1247 , C12N15/1068 , C12P19/34
Abstract: The present invention provides methods for large-scale production of a composition enriched for full-length mRNA molecules using an SP6 RNA polymerase and compositions produced using such methods and uses thereof.
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公开(公告)号:US10266843B2
公开(公告)日:2019-04-23
申请号:US15482431
申请日:2017-04-07
Applicant: Translate Bio, Inc.
Inventor: Frank DeRosa , Michael Heartlein , Daniel Crawford , Shrirang Karve
IPC: C07H21/02 , C07H21/04 , C12N15/85 , C07K14/505 , C12N9/10 , C12N9/02 , C07K14/705 , A61K48/00 , A61K38/17 , A61K38/44 , A61K38/45 , A61K38/18 , A61K31/7115 , A61K31/7125 , C12P21/00 , C12N15/67 , A01K67/00
Abstract: The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3′ ends such that the multimeric coding nucleic acid compound comprises two or more 5′ ends.
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公开(公告)号:US10975369B2
公开(公告)日:2021-04-13
申请号:US15907086
申请日:2018-02-27
Applicant: Translate Bio, Inc.
Inventor: Frank DeRosa , Michael Heartlein , Jonathan Abysalh , Daniel Crawford , Anusha Dias , Shrirang Karve
Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, a step of centrifuging an mRNA suspension in a centrifuge comprising a porous substrate at a speed sufficient to remove process contaminants and to precipitate purified mRNA composition onto the porous substrate.
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公开(公告)号:US11976272B2
公开(公告)日:2024-05-07
申请号:US17070877
申请日:2020-10-14
Applicant: Translate Bio, Inc.
Inventor: Jonathan Abysalh , Daniel Crawford , Frank DeRosa , Shrirang Karve , Anusha Dias , Michael Heartlein
IPC: C12N15/10 , B01L3/00 , C12Q1/6806
CPC classification number: C12N15/1017 , B01L3/502753 , C12Q1/6806 , B01L2200/028 , B01L2200/16 , B01L2300/0681 , B01L2400/0409 , C12Q2523/113 , C12Q2523/308 , C12Y201/03003
Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.
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公开(公告)号:US11560562B2
公开(公告)日:2023-01-24
申请号:US15907163
申请日:2018-02-27
Applicant: Translate Bio, Inc.
Inventor: Anusha Dias , Daniel Crawford , Frank DeRosa , Jonathan Abysalh , Michael Heartlein
IPC: C12N15/10 , C12P19/34 , C12N9/12 , G01N27/447
Abstract: The present invention provides methods for large-scale production of a composition enriched for full-length mRNA molecules using an SP6 RNA polymerase and compositions produced using such methods and uses thereof.
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公开(公告)号:US20220073944A1
公开(公告)日:2022-03-10
申请号:US17404283
申请日:2021-08-17
Applicant: TRANSLATE BIO, INC.
Inventor: Frank DeRosa , Michael Heartlein , Daniel Crawford , Shrirang Karve
IPC: C12N15/85 , C07K14/505 , C12N9/10 , C12N9/02 , C07K14/705 , A61K48/00 , A61K38/17 , A61K38/44 , A61K38/45 , A61K38/18 , A61K31/7115 , A61K31/7125 , C07H21/02 , C12N15/67 , C12P21/00
Abstract: The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3′ ends such that the multimeric coding nucleic acid compound comprises two or more 5′ ends.
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公开(公告)号:US20200172923A1
公开(公告)日:2020-06-04
申请号:US16540791
申请日:2019-08-14
Applicant: Translate Bio, Inc.
Inventor: Frank DeRosa , Michael Heartlein , Daniel Crawford , Shrirang Karve
IPC: C12N15/85 , C12P21/00 , C12N15/67 , C07H21/02 , A61K31/7125 , A61K31/7115 , A61K38/18 , A61K38/45 , A61K38/44 , A61K38/17 , A61K48/00 , C07K14/705 , C12N9/02 , C12N9/10 , C07K14/505
Abstract: The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3′ ends such that the multimeric coding nucleic acid compound comprises two or more 5′ ends.
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公开(公告)号:US11124804B2
公开(公告)日:2021-09-21
申请号:US16540791
申请日:2019-08-14
Applicant: Translate Bio, Inc.
Inventor: Frank DeRosa , Michael Heartlein , Daniel Crawford , Shrirang Karve
IPC: C07H21/02 , A61K31/7115 , A61K31/7125 , C12N15/85 , C07K14/505 , C12N9/10 , C12N9/02 , C07K14/705 , A61K48/00 , A61K38/17 , A61K38/44 , A61K38/45 , A61K38/18 , C12N15/67 , C12P21/00 , A01K67/00
Abstract: The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3′ ends such that the multimeric coding nucleic acid compound comprises two or more 5′ ends.
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