公开(公告)号：US5723125A

公开(公告)日：1998-03-03

申请号：US719331

申请日：1996-09-25

Applicant: Tse Wen Chang ,  Liming Yu

Inventor： Tse Wen Chang ,  Liming Yu

IPC: C12N15/00 ,  A61K38/21 ,  A61K39/395 ,  A61P1/16 ,  A61P31/12 ,  A61P35/00 ,  A61P37/02 ,  C07K14/56 ,  C07K16/00 ,  C07K19/00 ,  C12N5/10 ,  C12P21/02 ,  C12R1/19 ,  A61K39/00 ,  C07K1/00

CPC classification number: C07K14/56 ,  C07K19/00 ,  C07K2319/00

Abstract: Disclosed is a hybrid recombinant protein consisting of human interferon, preferably interferon-.alpha. (IFN.alpha.), and human immunoglobulin Fc fragment, preferably .gamma.4 chain, joined by a peptide linker comprising the sequence Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser (SEQ ID NO:1).

Abstract translation: 公开了由人干扰素，优选干扰素-α（IFNα）和人免疫球蛋白Fc片段（优选γ4链）组成的杂交重组蛋白，其通过包含序列Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser（SEQ ID NO：1）。

公开(公告)号：US20080163656A1

公开(公告)日：2008-07-10

申请号：US11620222

申请日：2007-01-05

Applicant: Tse Wen Chang

Inventor： Tse Wen Chang

IPC: E05B19/04

CPC classification number: E05B19/24 ,  Y10T70/7876

Abstract: Disclosed is a method of swiftly identifying by touch or sight (including in low light) different keys in a set. Different keys are tagged with protrusions from their surface, and identifiers for different keys may differ in the total number of protrusions (which can vary in arrangement patterns in accordance with the number of protrusions), the shapes of the protrusions, and/or the color of the protrusions. The protrusions are fluorescent to allow identification in low light or dark. The set of protrusions for each key can be adhered to the key by placing them on one side of a support strip, which is coated with adhesive on the other side.

Abstract translation: 公开了一种通过触摸或瞄准（包括低光）快速识别一组中的不同键的方法。 不同的键被标记有来自其表面的突起，并且不同键的标识符可以在突起的总数（根据突起的数量可以在布置图案中变化），突起的形状和/或颜色 的突起。 突起是荧光的，以允许在低光或黑暗中识别。 每个键的一组凸起可以通过将其放置在另一侧涂覆有粘合剂的支撑条的一侧上而被粘附到键上。

公开(公告)号：US6106835A

公开(公告)日：2000-08-22

申请号：US11130

申请日：1993-01-28

Applicant: Tse Wen Chang

Inventor： Tse Wen Chang

IPC: A61K9/127 ,  A61K9/16 ,  A61K38/00 ,  A61K47/48 ,  C07K16/28 ,  A61K39/40

CPC classification number: A61K47/48823 ,  A61K47/48561 ,  A61K9/1271 ,  A61K9/167 ,  C07K16/28 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  A61K38/00 ,  C07K2317/74

Abstract: Several forms of immunoregulatory substances are derived from monoclonal antibodies (MAbs) that are specific for a T or B cell surface antigen, such as CD3, TCR, CD4, or CD8 on T cells or membrane-bound immunoglobulins on B cells. The substances include: a mixture of F(ab').sub.2 fragments (or other divalent binding molecules which lack Fc) which each bind noncompetitively to different monovalent antigenic epitopes on the same antigen; the F(ab').sub.2 fragment (or other divalent binding molecules which lack Fc) of a bispecific antibody which has each of its binding sites derived from one of the two MAbs that bind noncompetitively to monovalent antigenic epitopes on the same antigen; a conjugate including a polymeric backbone, such as polyethylene glycol ("PEG"), cellulose, dextran, agarose, or an amino acid copolymer or a liposome, that is coupled with the binding molecules, e.g., Fv, Fab, or F(ab').sub.2, which bind noncompetitively to monovalent antigenic epitopes on the same antigen.

Abstract translation: 免疫调节物质的几种形式来源于T细胞表面抗原如T细胞上的CD3，TCR，CD4或CD8或B细胞膜结合的免疫球蛋白特异的单克隆抗体（MAb）。 这些物质包括：F（ab'）2片段（或缺乏Fc的其他二价结合分子）的混合物，其各自不依赖于相同抗原上不同的单价抗原表位结合; 双特异性抗体的F（ab'）2片段（或缺少Fc的其它二价结合分子），其具有各自的结合位点，其衍生自两个MAb中的一个与同一抗原上的单价抗原表位结合; 包含聚合物主链，例如聚乙二醇（“PEG”），纤维素，葡聚糖，琼脂糖或氨基酸共聚物或脂质体，其与结合分子例如Fv，Fab或F（ab '）2，其在相同抗原上非竞争性地结合于单价抗原表位。

公开(公告)号：US20080189996A1

公开(公告)日：2008-08-14

申请号：US12051199

申请日：2008-03-19

Applicant: Tse Wen Chang

Inventor： Tse Wen Chang

IPC: G09F3/00

CPC classification number: E05B19/24 ,  Y10T70/7876

Abstract: Disclosed is a method of swiftly identifying by touch or sight (including in low light) different keys in a set. Different keys are tagged with protrusions from their surface, and identifiers for different keys may differ in the total number of protrusions (which can vary in arrangement patterns in accordance with the number of protrusions), the shapes of the protrusions, and/or the color of the protrusions. The protrusions are fluorescent to allow identification in low light or dark. The set of protrusions for each key can be adhered to the key by placing them on one side of a support strip, which is coated with adhesive on the other side.

Abstract translation: 公开了一种通过触摸或瞄准（包括低光）快速识别一组中的不同键的方法。 不同的键被标记有来自其表面的突起，并且不同键的标识符可以在突起的总数（根据突起的数量可以在布置图案中变化），突起的形状和/或颜色 的突起。 突起是荧光的，以允许在低光或黑暗中识别。 每个键的一组凸起可以通过将其放置在另一侧涂覆有粘合剂的支撑条的一侧上而被粘附到键上。

公开(公告)号：US06685930B1

公开(公告)日：2004-02-03

申请号：US08855744

申请日：1997-05-08

Applicant: Tse Wen Chang

Inventor： Tse Wen Chang

IPC: A61K4500

CPC classification number: C07K16/468 ,  A61K9/1271 ,  A61K38/00 ,  A61K47/6879 ,  A61K47/6893 ,  A61K47/6913 ,  B82Y5/00 ,  C07K2319/00

Abstract: Disclosed is a method of using bifunctional binding molecules, such as two linked VH-VL single chain binding molecules, to recruit a therapeutic agent to a solid tissue site. The therapeutic agent is administered separately from the binding molecules and following the administration of a remover substance which aids in clearing free binding molecules in the circulation. In the preferred mode of the invention, the binding molecules have one specificity for antigens at the target site and one for the therapeutic agent. The binding molecules are administered and allowed time to approach a maximum concentration in the extravascular space. A remover substance, preferably a liposome conjugated with antibodies which are reactive with an antigenic epitope on the binding molecules, is then administered to remove excess binding molecules from the circulation and the extravascular space. A therapeutic agent, preferably a cytotoxic drug such as ricin A chain modified so as to enable it to enter the target cells once delivered to the target site, is then administered.

Abstract translation: 公开了使用双功能结合分子（例如两个连接的VH-VL单链结合分子）将治疗剂募集到固体组织部位的方法。 治疗剂与结合分子分开施用，并且在施用有助于清除循环中的游离结合分子的去除剂物质后。 在本发明的优选方式中，结合分子对靶位点的抗原具有一个特异性，而对治疗剂具有一个特异性。 施用结合分子并允许时间接近血管外空间的最大浓度。 然后施用去除剂物质，优选与抗体结合的脂质体，其与结合分子上的抗原表位反应，以从循环和血管外空间中除去过量的结合分子。 然后施用治疗剂，优选细胞毒性药物如蓖麻毒蛋白A链修饰，以使其能够一旦递送至靶位点进入靶细胞。

公开(公告)号：US06309880B1

公开(公告)日：2001-10-30

申请号：US08089990

申请日：1993-07-09

Applicant: Tse Wen Chang ,  Michael S. C. Fung ,  Bill N. C. Sun ,  Cecily R. Y. Sun ,  Nancy T. Chang

Inventor： Tse Wen Chang ,  Michael S. C. Fung ,  Bill N. C. Sun ,  Cecily R. Y. Sun ,  Nancy T. Chang

IPC: C12N506

CPC classification number: C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K47/6425 ,  A61K47/6839 ,  C07K16/1063 ,  C07K2317/34 ,  C12N2740/16122

Abstract: A particular epitope located within the CD4-binding region of gpl20 of HIV-1, and antibodies specific for the epitope which can inhibit HIV-1 infection of human cells by diverse strains and isolates of the virus, is disclosed. The antibodies are useful for a number of purposes, including diagnosis of HIV-1 infection.

Abstract translation: 公开了位于HIV-1的gp120的CD4结合区域内的特定表位，以及能够通过多种毒株和病毒分离株抑制人细胞HIV-1感染的表位的特异抗体。 抗体可用于许多目的，包括HIV-1感染的诊断。

公开(公告)号：US06197298B1

公开(公告)日：2001-03-06

申请号：US08035723

申请日：1993-03-23

Applicant: Tse Wen Chang

Inventor： Tse Wen Chang

IPC: A61K3940

CPC classification number: C07K16/2809 ,  A61K2039/505

Abstract: Several forms of immunoregulatory substances are derived from monoclonal antibodies (MAbs) that are specific for a T cell surface antigen, such as CD3, TCR, CD4, or CD8 on T cells. The substances include: a mixture of F(ab′)2 fragments (or other divalent binding molecules which lack Fc) which each bind noncompetitively to different monovalent antigenic epitopes on the same antigen; the F(ab′)2 fragment (or other divalent binding molecules which lack Fc) of a bispecific antibody which has each of its binding sites derived from one of the two MAbs that bind noncompetitively to monovalent antigenic epitopes on the same antigen; a conjugate including a polymeric backbone, such as polyethylene glycol (“PEG”), cellulose, dextran, agarose, or an amino acid copolymer or a liposome, that is coupled with the binding molecules, e.g., Fv, Fab, or F(ab′)2, which bind noncompetitively to monovalent antigenic epitopes on the same antigen.

Abstract translation: 免疫调节物质的几种形式来自T细胞表面抗原如CD3，TCR，CD4或CD8特异性的单克隆抗体（MAb）。 这些物质包括：F（ab'）2片段（或缺乏Fc的其他二价结合分子）的混合物，其各自不依赖于相同抗原上不同的单价抗原表位结合; 双特异性抗体的F（ab'）2片段（或缺少Fc的其它二价结合分子），其具有各自的结合位点，其衍生自两个MAb中的一个与同一抗原上的单价抗原表位结合; 包含聚合物主链，例如聚乙二醇（“PEG”），纤维素，葡聚糖，琼脂糖或氨基酸共聚物或脂质体，其与结合分子例如Fv，Fab或F（ab '）2，其在相同抗原上非竞争性地结合于单价抗原表位。

公开(公告)号：US5872222A

公开(公告)日：1999-02-16

申请号：US993291

申请日：1992-12-18

Applicant: Tse Wen Chang

Inventor： Tse Wen Chang

IPC: A61K9/127 ,  A61K9/16 ,  A61K38/00 ,  A61K47/48 ,  C07K16/28 ,  C07K16/00

CPC classification number: A61K9/1271 ,  A61K47/48561 ,  A61K47/48823 ,  A61K9/167 ,  C07K16/28 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  A61K38/00

Abstract: Disclosed are conjugates including a substantially nonimmunogenic polymer backbone or microbead and binding molecules, such as Fv, Fab, or F(ab').sub.2 fragments of monoclonal antibodies or whole antibodies that are bound through their Fc carbohydrate moieties or have their Fc portion modified so that they cannot effect ADCC or complement-mediated cytolysis, and that are specific for a T cell surface antigen, such as CD3, TCR, CD4, CD8, or CD28 on T cells. The polymer or microbead is preferably made of cross-linked dextran, ficoll, latex, or agarose, and is preferably of 0.1 to 10 .mu.m in size, so that it can be suspended in fluids for in vivo applications. These conjugates can be used as adjuvants to enhance the antibody response against an administered immunogen.

Abstract translation: 公开了包括基本上非免疫原性的聚合物骨架或微珠和结合分子（例如单克隆抗体的Fv，Fab或F（ab'）2）片段或通过其Fc碳水化合物部分结合或将其Fc部分修饰的整个抗体的结合物 它们不能影响ADCC或补体介导的细胞溶解，并且对于T细胞上的T细胞表面抗原如CD3，TCR，CD4，CD8或CD28是特异性的。 聚合物或微珠优选由交联的葡聚糖，ficoll，胶乳或琼脂糖制成，并且其尺寸优选为0.1-10μm，从而可将其悬浮在用于体内应用的流体中。 这些缀合物可以用作佐剂以增强针对施用的免疫原的抗体应答。

公开(公告)号：US5670626A

公开(公告)日：1997-09-23

申请号：US263258

申请日：1994-06-21

Applicant: Tse Wen Chang

Inventor： Tse Wen Chang

IPC: C12N15/09 ,  A61K38/00 ,  A61K39/395 ,  A61K47/48 ,  A61P37/08 ,  C07K16/16 ,  C07K16/18 ,  C07K17/10 ,  C12P21/08 ,  C07K16/14

CPC classification number: C07K16/18 ,  A61K47/48546 ,  A61K38/00

Abstract: Disclosed are pharmaceutical preparations containing human monoclonal IgA antibodies specific for major allergenic proteins found in ragweed, house dust mites, and cat and dog dander. Also disclosed are constructs comprising physiological compatible polymer backbones or microbeads and a plurality of covalently conjugated allergen-specific binding molecules. Such binding molecules are IgG or IgA, or their F(ab').sub.2, Fab, or Fv fragments, specific to the major allergenic proteins mentioned above. Also disclosed are methods for treating a patient with allergic rhinitis, asthma, or conjunctivitis by applying a pharmaceutical preparation containing the antibodies specific for the allergenic molecules, to which the patient is sensitized, to the patient's affected mucosal tissues, such as the nasal linings, the respiratory tract, or the eyes.

Abstract translation: 公开了含有针对豚草，房尘螨和猫和狗皮屑中发现的主要过敏原蛋白特异性的人单克隆IgA抗体的药物制剂。 还公开了包含生理相容的聚合物主链或微珠和多个共价偶联的变应原特异性结合分子的构建体。 这样的结合分子是对上述主要变应原蛋白特异的IgG或IgA或其F（ab'）2，Fab或Fv片段。 还公开了通过将患有致敏的致敏性分子的特异性抗体的药物制剂施用于患者的受影响的粘膜组织（例如鼻内衬）来治疗患有过敏性鼻炎，哮喘或结膜炎的患者的方法， 呼吸道或眼睛。

公开(公告)号：US20140212500A1

公开(公告)日：2014-07-31

申请号：US14239924

申请日：2012-08-23

Applicant: Tse Wen Chang

Inventor： Tse Wen Chang

IPC: A61K39/385 ,  A61K39/00 ,  A23L1/30 ,  A61K9/14

CPC classification number: A61K39/385 ,  A23L33/10 ,  A61K9/14 ,  A61K39/00 ,  A61K39/0002 ,  A61K39/0003 ,  A61K39/35 ,  A61K39/36

Abstract: Compositions containing sterilized antigens with a high diversity, which can be collected from primitive jungle areas, and uses thereof for balancing immune responses and treating immunological diseases in a subject.

Abstract translation: 含有高度多样性的灭菌抗原的组合物，其可以从原始丛林地区收集，以及其用于平衡免疫应答和治疗受试者的免疫疾病的用途。