公开(公告)号：US20050266568A1

公开(公告)日：2005-12-01

申请号：US11101658

申请日：2005-04-08

申请人： Tsuyoshi Fujihara ,  Shozo Fujita ,  Shunsaku Takeishi ,  Kenji Arinaga ,  Yoshitaka Yamaguchi ,  Tatsuya Usuki ,  Ulrich Rant ,  Karin Buchholz ,  Marc Tornow

发明人： Tsuyoshi Fujihara ,  Shozo Fujita ,  Shunsaku Takeishi ,  Kenji Arinaga ,  Yoshitaka Yamaguchi ,  Tatsuya Usuki ,  Ulrich Rant ,  Karin Buchholz ,  Marc Tornow

IPC分类号： A61K48/00 ,  A61N1/30 ,  C12M1/00 ,  C12N5/10 ,  C12N15/87 ,  C12Q1/68

CPC分类号： A61K48/0083

摘要： A molecule-releasing apparatus includes a releasing unit configured to release molecules, wherein the releasing unit comprises at least one conductive member and releases molecules electrically interacting with the conductive member to which a potential is applied, from the conductive member by changing the potential so as to remove the interaction. In a moleculue-releasing method, molecules electrically attracted to two or more conductive members are released by electrical repulsion from the conductive members at different times. The molecule-releasing apparatus and molecule-releasing method are capable of efficiently releasing or transferring various useful molecules such as DNA molecules, into targets such as cells and can be safely applied for use in gene therapy and other applications.

摘要翻译： 分子释放装置包括配置成释放分子的释放单元，其中释放单元包括至少一个导电构件，并且通过改变电位从导电构件释放与施加电位的导电构件电相互作用的分子，从而 去除互动。 在分子释放方法中，电吸引到两个或更多导电构件的分子在不同时间通过电导电从导电构件释放。 分子释放装置和分子释放方法能够有效地将诸如DNA分子的各种有用分子释放或转移到诸如细胞的靶中，并且可以安全地应用于基因治疗和其它应用中。

公开(公告)号：US20060003437A1

公开(公告)日：2006-01-05

申请号：US11223924

申请日：2005-09-13

申请人： Tsuyoshi Fujihara ,  Shozo Fujita ,  Shunsaku Takeishi ,  Kenji Arinaga ,  Yoshitaka Yamaguchi ,  Tatsuya Usuki

发明人： Tsuyoshi Fujihara ,  Shozo Fujita ,  Shunsaku Takeishi ,  Kenji Arinaga ,  Yoshitaka Yamaguchi ,  Tatsuya Usuki

IPC分类号： C12M3/00

CPC分类号： C12Q1/6816 ,  C12Q1/6825 ,  C12Q2565/607 ,  C12Q2565/107 ,  C12Q2523/308

摘要： The present invention provides, for example, a target detecting device comprising a target capturer, means for releasing the target capturer, light irradiating means and light detecting means, the target capturer at least partially containing a region interactive with an electrically conductive member, being capable of capturing a target, and being capable of emitting light upon irradiation with light in the case of not interacting with the electrically conductive member, the means for releasing the target capturer serving to release the target capturer from the electrically conductive member by ceasing the interaction between the target capturer and the electrically conductive member, the light irradiating means serving to apply light to the electrically conductive member, and the light detecting means serving to detect light emitted by the target capturer upon irradiation of light applied by the light irradiating means. It also provides a target capturer comprising an interacting section, a capturing section and a light emitting section, the interacting section at least partially containing a region interactive with an electrically conductive member, the capturing section capable of capturing a target, and the light emitting section capable of emitting light upon irradiation with light when the region in the interacting section does not interact with the electrically conductive member.

摘要翻译： 本发明提供例如包括目标捕获器的目标检测装置，用于释放目标捕获器的装置，光照射装置和光检测装置，目标捕获器至少部分地包含与导电构件交互的区域，能够 捕获目标，并且在不与导电构件相互作用的情况下能够在照射光时发光，所述用于释放目标捕获器的装置用于通过停止导电构件之间的相互作用而释放目标捕获器 目标捕获器和导电构件，用于向导电构件施加光的光照射装置，以及用于在由照射装置施加的光照射时检测由目标捕获器发射的光的光检测装置。 它还提供一种包括相互作用部分，捕获部分和发光部分的目标捕获器，所述相互作用部分至少部分地包含与导电部件交互的区域，所述捕获部分能够捕获目标，并且所述发光部分 当相互作用部分中的区域不与导电构件相互作用时能够用光照射发光。

公开(公告)号：US20050069932A1

公开(公告)日：2005-03-31

申请号：US10940746

申请日：2004-09-15

申请人： Kenji Arinaga ,  Ulrich Rant ,  Shozo Fujita ,  Tsuyoshi Fujihara ,  Marc Tornow ,  Gerhard Abstreiter

发明人： Kenji Arinaga ,  Ulrich Rant ,  Shozo Fujita ,  Tsuyoshi Fujihara ,  Marc Tornow ,  Gerhard Abstreiter

IPC分类号： G01N33/543 ,  C12Q1/68 ,  G01N21/64 ,  G01N21/78 ,  G01N33/53 ,  G01N37/00 ,  C12M1/34 ,  G01J3/30

CPC分类号： G01N21/6452 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N2021/6441

摘要： In an analyte evaluating device comprising a carrier body that can be bound with an analyte having a fluorescence-labeled part that can emit fluorescence by light received when the distance between the fluorescence-labeled part and the carrier body is enlarged, at least one factor selected from the group consisting of a light irradiation angle, a light irradiation intensity, a light irradiation area, a fluorescence detection angle, a fluorescence detection area, the shape of the carrier body, the surface area of the carrier body, a salt concentration in a medium for use in the detection, and the adhesion density of analytes on the carrier body, is made to be adjustable. A high sensitivity is realized. Evaluation is possible without introducing fluorescence-labeled parts. Evaluation for a tiny amount of sample is possible. It is also possible to evaluate objects in a mixed state. Miniaturized, complex, and integrated devices are possible.

摘要翻译： 在分析物评价装置中，所述分析物评估装置包括载体主体，所述载体主体可以与具有荧光标记部分的分析物结合，所述分析物可以在荧光标记部分和载体体之间的距离被扩大时由接收到的光发射荧光，选择至少一个因素 由照射照射角度，光照射强度，光照射面积，荧光检测角度，荧光检测范围，载体的形状，载体的表面积， 用于检测的介质和载体上分析物的粘附密度被调整。 实现了高灵敏度。 评估是可能的，而不引入荧光标记的部分。 可以对少量样品进行评估。 也可以评估混合状态下的对象。 小型化，复杂和集成的设备是可能的。

公开(公告)号：US20070048760A1

公开(公告)日：2007-03-01

申请号：US11430045

申请日：2006-05-09

申请人： Kenji Arinaga ,  Ulrich Rant ,  Tsuyoshi Fujihara ,  Shozo Fujita

发明人： Kenji Arinaga ,  Ulrich Rant ,  Tsuyoshi Fujihara ,  Shozo Fujita

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M3/00

CPC分类号： G01N33/5438 ,  B01J2219/00605 ,  C12Q2527/137 ,  C12Q2527/143 ,  C12Q2565/518 ,  G01N33/543

摘要： A device for quantitatively determining an analyte is provided to conspicuously improve the performance of the quantitative determination. This device is equipped with a flow channel, an analyte detecting unit for capturing and detecting the analyte, and a quantitative measurement unit for quantitatively determining the analyte, wherein a signal generated when the analyte detecting unit has detected the analyte is divided into a plurality of parts in the direction of the flow in the flow channel at the quantitative measurement unit for processing. Also provided are technologies including one for controlling the density of molecules attached to the surface of a solid. In these technologies, when molecules are attached to a substrate, the density of attached molecules is controlled, by having an electrolyte also present in a solution containing the molecules to adjust the screening effect by the electrolyte, and by taking into consideration the effective size of a molecule.

摘要翻译： 提供了一种用于定量测定分析物的装置，以显着提高定量测定的性能。 该装置配备有流路，用于捕获和检测分析物的分析物检测单元和用于定量测定分析物的定量测量单元，其中当分析物检测单元检测到分析物时产生的信号被分成多个 在定量测量单元处的流动通道中的流动方向上的部件进行处理。 还提供了包括用于控制附着在固体表面上的分子的密度的技术。 在这些技术中，当分子连接到基底上时，通过在含有分子的溶液中也存在电解质来调节附着分子的密度，以调节电解质的筛选效果，并考虑到有效尺寸 一个分子

公开(公告)号：US20060040378A1

公开(公告)日：2006-02-23

申请号：US11036367

申请日：2005-01-18

申请人： Kenji Arinaga ,  Ulrich Rant ,  Tsuyoshi Fujihara ,  Shozo Fujita

发明人： Kenji Arinaga ,  Ulrich Rant ,  Tsuyoshi Fujihara ,  Shozo Fujita

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/34

CPC分类号： G01N33/5438 ,  B01J2219/00605 ,  C12Q2527/137 ,  C12Q2527/143 ,  C12Q2565/518 ,  G01N33/543

摘要： A device for quantitatively determining an analyte is provided to conspicuously improve the performance of the quantitative determination. This device is equipped with a flow channel, an analyte detecting unit for capturing and detecting the analyte, and a quantitative measurement unit for quantitatively determining the analyte, wherein a signal generated when the analyte detecting unit has detected the analyte is divided into a plurality of parts in the direction of the flow in the flow channel at the quantitative measurement unit for processing. Also provided are technologies including one for controlling the density of molecules attached to the surface of a solid. In these technologies, when molecules are attached to a substrate, the density of attached molecules is controlled, by having an electrolyte also present in a solution containing the molecules to adjust the screening effect by the electrolyte, and by taking into consideration the effective size of a molecule.

公开(公告)号：US20110100822A1

公开(公告)日：2011-05-05

申请号：US12923588

申请日：2010-09-29

申请人： Kenji Arinaga ,  Ulrich Rant ,  Tsuyoshi Fujihara ,  Shozo Fujita

发明人： Kenji Arinaga ,  Ulrich Rant ,  Tsuyoshi Fujihara ,  Shozo Fujita

IPC分类号： G01N27/447 ,  C12Q1/37

CPC分类号： G01N33/5438 ,  B01J2219/00605 ,  C12Q2527/137 ,  C12Q2527/143 ,  C12Q2565/518 ,  G01N33/543

摘要： A device for quantitatively determining an analyte is provided to conspicuously improve the performance of the quantitative determination. This device is equipped with a flow channel, an analyte detecting unit for capturing and detecting the analyte, and a quantitative measurement unit for quantitatively determining the analyte, wherein a signal generated when the analyte detecting unit has detected the analyte is divided into a plurality of parts in the direction of the flow in the flow channel at the quantitative measurement unit for processing. Also provided are technologies including one for controlling the density of molecules attached to the surface of a solid. In these technologies, when molecules are attached to a substrate, the density of attached molecules is controlled, by having an electrolyte also present in a solution containing the molecules to adjust the screening effect by the electrolyte, and by taking into consideration the effective size of a molecule.

摘要翻译： 提供了一种用于定量测定分析物的装置，以显着提高定量测定的性能。 该装置配备有流路，用于捕获和检测分析物的分析物检测单元和用于定量测定分析物的定量测量单元，其中当分析物检测单元检测到分析物时产生的信号被分成多个 在定量测量单元处的流动通道中的流动方向上的部件进行处理。 还提供了包括用于控制附着在固体表面上的分子的密度的技术。 在这些技术中，当分子连接到基底上时，通过在含有分子的溶液中也存在电解质来调节附着分子的密度，以调节电解质的筛选效果，并考虑到有效尺寸 一个分子

公开(公告)号：US07311889B2

公开(公告)日：2007-12-25

申请号：US10464847

申请日：2003-06-19

申请人： Yuji Awano ,  Yoshitaka Yamaguchi ,  Kenji Arinaga ,  Shozo Fujita

发明人： Yuji Awano ,  Yoshitaka Yamaguchi ,  Kenji Arinaga ,  Shozo Fujita

IPC分类号： D01F9/12

CPC分类号： D01F9/12 ,  B82Y30/00 ,  D01F9/127 ,  D01F9/1272 ,  D01F9/133 ,  Y10S977/843

摘要： The invention provides a process for production of carbon nanotubes whereby a laminate prepared by alternating lamination of a metal catalyst and a material other than the metal catalyst is cut to expose the laminated structure, and carbon nanotubes are grown on the metal catalyst at the cut surface of the laminate. The process results in high-quality carbon nanotubes, with minimized bundle growth, which are each individually and independently arranged in a highly precise manner at prescribed locations.The invention also provides a carbon nanotube production process comprising a step of preparing a substrate which is inclined in one or two dimensions from a specific highly symmetrical crystal orientation and vapor depositing a metal catalyst along the atomic steps appearing on the surface of the substrate, and a step of growing the carbon nanotubes by chemical vapor deposition (CVD) using the metal catalyst as nuclei. It is thus possible to control the growth locations, diameters, orientation and chirality of carbon nanotubes.

摘要翻译： 本发明提供一种碳纳米管的制造方法，其中通过交替层压金属催化剂和除了金属催化剂之外的材料制成的层压体被切割以暴露层压结构，并且碳纳米管在切割表面上在金属催化剂上生长 的层压板。 该方法产生高质量的碳纳米管，其最小化束生长，它们在规定的位置上以高度精确的方式各自独立地排列。 本发明还提供一种碳纳米管制造方法，其包括以下步骤：制备从特定的高度对称的晶体取向倾斜一维或二维的衬底，并沿着出现在衬底表面上的原子台阶气相沉积金属催化剂，以及 通过使用金属催化剂作为核的化学气相沉积（CVD）来生长碳纳米管的步骤。 因此可以控制碳纳米管的生长位置，直径，取向和手性。

公开(公告)号：US20070253889A1

公开(公告)日：2007-11-01

申请号：US10464847

申请日：2003-06-19

申请人： Yuji Awano ,  Yoshitaka Yamaguchi ,  Kenji Arinaga ,  Shozo Fujita

发明人： Yuji Awano ,  Yoshitaka Yamaguchi ,  Kenji Arinaga ,  Shozo Fujita

IPC分类号： D01F9/12

CPC分类号： D01F9/12 ,  B82Y30/00 ,  D01F9/127 ,  D01F9/1272 ,  D01F9/133 ,  Y10S977/843

摘要： The invention provides a process for production of carbon nanotubes whereby a laminate prepared by alternating lamination of a metal catalyst and a material other than the metal catalyst is cut to expose the laminated structure, and carbon nanotubes are grown on the metal catalyst at the cut surface of the laminate. The process results in high-quality carbon nanotubes, with minimized bundle growth, which are each individually and independently arranged in a highly precise manner at prescribed locations. The invention also provides a carbon nanotube production process comprising a step of preparing a substrate which is inclined in one or two dimensions from a specific highly symmetrical crystal orientation and vapor depositing a metal catalyst along the atomic steps appearing on the surface of the substrate, and a step of growing the carbon nanotubes by chemical vapor deposition (CVD) using the metal catalyst as nuclei. It is thus possible to control the growth locations, diameters, orientation and chirality of carbon nanotubes.

摘要翻译： 本发明提供一种碳纳米管的制造方法，其中通过交替层压金属催化剂和除了金属催化剂之外的材料制成的层压体被切割以暴露层压结构，并且碳纳米管在切割表面上在金属催化剂上生长 的层压板。 该方法产生高质量的碳纳米管，其最小化束生长，它们在规定的位置上以高度精确的方式各自独立地排列。 本发明还提供一种碳纳米管制造方法，其包括以下步骤：制备从特定的高度对称的晶体取向倾斜一维或二维的衬底，并沿着出现在衬底的表面上的原子台阶气相沉积金属催化剂，以及 通过使用金属催化剂作为核的化学气相沉积（CVD）来生长碳纳米管的步骤。 因此可以控制碳纳米管的生长位置，直径，取向和手性。

公开(公告)号：US08568966B2

公开(公告)日：2013-10-29

申请号：US11700264

申请日：2007-01-31

申请人： Kenji Arinaga ,  Ulrich Rant ,  Erika Pringsheim ,  Jelena Knezevic

发明人： Kenji Arinaga ,  Ulrich Rant ,  Erika Pringsheim ,  Jelena Knezevic

IPC分类号： C12Q1/00

CPC分类号： G01N33/54353

摘要： An object of the present invention is to provide a technique that can adjust a molecular density of the film of functional molecules (e.g. DNA molecules), which is utilized for biochips such as DNA chip, to a desired degree efficiently and easily. The method for producing a molecular film with an adjusted density according to the present invention includes forming a molecular film and adjusting a molecular density. In the forming a molecular film, a molecular film composed of molecules is formed on a conductive substrate, wherein the molecule includes a region capable of binding to the conductive substrate at least in a portion thereof. In the density adjusting, a molecular density of the molecular film is adjusted by desorbing a part of the molecules which make up the molecular film from the conductive substrate.

摘要翻译： 本发明的一个目的是提供一种可以有效且容易地调整用于生物芯片如DNA芯片的功能分子（例如DNA分子）的膜的分子密度达到期望的程度的技术。 根据本发明的具有调节密度的分子膜的制备方法包括形成分子膜并调节分子密度。 在形成分子膜时，在导电性基板上形成由分子构成的分子膜，其中，所述分子具有能够至少部分地与导电性基板结合的区域。 在密度调节中，分子膜的分子密度通过从导电性基材上解吸构成分子膜的一部分分子来调节。

公开(公告)号：US08445262B2

公开(公告)日：2013-05-21

申请号：US12239987

申请日：2008-09-29

申请人： Kenji Arinaga ,  Ulrich Rant ,  Simon Scherer ,  Erika Pringsheim

发明人： Kenji Arinaga ,  Ulrich Rant ,  Simon Scherer ,  Erika Pringsheim

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： G01N21/6428 ,  G01N33/5438 ,  G01N2021/6432 ,  Y10T436/143333

摘要： In an analyte evaluation method for evaluating an analyte, AC voltage is applied between a substrate electrode on a substrate and a counter electrode, and signals obtained from a marker provided on an analyte bound to the substrate electrode are observed, wherein the frequency of the AC voltage is changed and the behavior of the average value of the marker signals is observed. A novel, highly-selective, low-noise method of evaluating a object of evaluation is thus achieved.

摘要翻译： 在用于评价分析物的分析物评价方法中，在基板上的基板电极和对置电极之间施加AC电压，并且观察到从与基板电极结合的分析物上提供的标记获得的信号，其中AC的频率 电压被改变，并且观察到标记信号的平均值的行为。 从而实现了评价评价对象的新颖，高选择性，低噪声的方法。