公开(公告)号：US20230175062A1

公开(公告)日：2023-06-08

申请号：US18062966

申请日：2022-12-07

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Jeremy LACKEY ,  Andres FERNANDEZ ,  David DODD ,  Alexander GORYAYNOV

IPC: C12Q1/6874 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2300/0645 ,  B01L2200/0652 ,  B01L2300/0819

Abstract: Provided herein are compositions, devices, systems and methods for single molecule sensing. Further provided are devices for nucleic acid sequencing. The compositions, devices, systems and methods described herein provide improved retrieval of biomolecule-based information.

公开(公告)号：US20220032256A1

公开(公告)日：2022-02-03

申请号：US17368697

申请日：2021-07-06

Applicant: Twist Bioscience Corporation

Inventor： Jeremy LACKEY ,  Scott INDERMUEHLE ,  David DODD ,  Stefan PITSCH

IPC: B01J19/00

Abstract: Provided herein are compositions, devices, systems and methods for generation and use of biomolecule-based information for storage. Further provided are devices comprising addressable LED arrays to control polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved storage, density, and retrieval of biomolecule-based information.

公开(公告)号：US20240344057A1

公开(公告)日：2024-10-17

申请号：US18647281

申请日：2024-04-26

Applicant: Twist Bioscience Corporation

Inventor： Jeremy LACKEY ,  David DODD

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/1093 ,  C12N15/102 ,  C12N15/113 ,  C12N2310/315 ,  C12N2320/33

Abstract: Disclosed herein are methods and compositions comprising a polymerase and a phosphorylated nucleoside, wherein the polymerase and the nucleoside are covalently linked by a cleavable linker at the terminal phosphate group. Further disclosed herein are enzymatic polynucleotide synthesis using polymerase and nucleotide conjugation strategies.

公开(公告)号：US20230158469A1

公开(公告)日：2023-05-25

申请号：US18051472

申请日：2022-10-31

Applicant: Twist Bioscience Corporation

Inventor： Jeremy LACKEY ,  David DODD ,  Stefan PITSCH

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00722

Abstract: Provided herein are compositions, devices, systems and methods for DNA oligomer synthesis. Further provided are devices comprising addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.). The compositions, devices, systems and methods described herein provide improved synthesis, storage, density, and retrieval of biomolecule-based information.

公开(公告)号：US20220145289A1

公开(公告)日：2022-05-12

申请号：US17504358

申请日：2021-10-18

Applicant: Twist Bioscience Corporation

Inventor： Jeremy LACKEY ,  David DODD

IPC: C12N15/10 ,  C12N15/113

Abstract: Disclosed herein are methods and compositions comprising a polymerase and a phosphorylated nucleoside, wherein the polymerase and the nucleoside are covalently linked by a cleavable linker at the terminal phosphate group. Further disclosed herein are enzymatic polynucleotide synthesis using polymerase and nucleotide conjugation strategies.