公开(公告)号：US20250057951A1

公开(公告)日：2025-02-20

申请号：US18719780

申请日：2022-12-16

Applicant: UNIVERSITÄT BASEL ,  CIMEIO THERAPEUTICS AG

Inventor： Stefanie URLINGER ,  Rosalba LEPORE ,  Lukas JEKER ,  Amélie WIEDERKEHR ,  Alessandro SINOPOLI ,  Anna CAMUS ,  Lisa WELLINGER ,  Romina MATTER-MARONE

IPC: A61K39/00

Abstract: The present disclosure relates to the use of cells having discernible surface protein with engineered or naturally occurring mutation(s) but functional surface protein for use in therapy. The present invention also relates to the use of cells having discernible CD117 surface protein variants but functional surface protein for use in therapy, in particular adoptive cell therapy.

公开(公告)号：US20220177882A1

公开(公告)日：2022-06-09

申请号：US17426385

申请日：2020-01-31

Applicant: UNIVERSITÄT BASEL

Inventor： Lukas JEKER ,  Marianne DÖLZ

IPC: C12N15/113 ,  A61K35/17 ,  A61P35/00 ,  C12N15/87

Abstract: The present invention relates to immune cells in which the regulatory activity of miR-17˜92 cluster or paralogs thereof is increased to confer calcineurin inhibitor resistance. In particular said immune cell is engineered to overexpress at least one mi RNA of miR-17˜92 cluster or paralogs thereof or to inactivate at least one miR-17˜92 cluster target gene to confer calcineurin inhibitor resistance. Particularly, the present invention relates to the use of calcineurin inhibitor-resistant immune cells in combination with calcineurin inhibitor in adoptive cell transfer therapy in a patient in need thereof.

公开(公告)号：US20230193320A1

公开(公告)日：2023-06-22

申请号：US17948313

申请日：2022-09-20

Applicant: UNIVERSITÄT BASEL

Inventor： Mara KORNETE ,  Lukas JEKER

IPC: C12N15/90 ,  C12N15/10 ,  A61P35/00 ,  A61K35/17 ,  A61K35/28 ,  C12N5/0783 ,  C12N5/0789

CPC classification number: C12N15/902 ,  C12N15/102 ,  A61P35/00 ,  A61K35/17 ,  A61K35/28 ,  C12N5/0636 ,  C12N5/0647

Abstract: The invention relates to a method to determine a homology directed repair (HDR) event within a eukaryotic cell, wherein the cell expresses a first isoform of a surface protein, which is different from a second isoform of said surface protein with regard to an amino acid marker. The method comprises the steps of inducing a DNA double strand break, providing a HDR template DNA construct comprising the amino acid marker corresponding to the second isoform of the surface protein and subsequently determining the expression of the first or second isoform of said surface protein on said cell, wherein expression of the second isoform indicates a successful HDR event. The invention also relates to a method for editing a genomic location of interest within a eukaryotic cell, and to a method of selectively depleting or enriching an edited cell in a composition of non-edited and edited cells.

公开(公告)号：US20190136263A1

公开(公告)日：2019-05-09

申请号：US16096074

申请日：2017-04-25

Applicant: UNIVERSITÄT BASEL

Inventor： Mara KORNETE ,  Lukas JEKER

IPC: C12N15/90 ,  A61K35/28 ,  A61K35/17 ,  A61P35/00 ,  C12N5/0789 ,  C12N5/0783

Abstract: The invention relates to a method to determine a homology directed repair (HDR) event within a eukaryotic cell, wherein the cell expresses a first isoform of a surface protein, which is different from a second isoform of said surface protein with regard to an amino acid marker. The method comprises the steps of inducing a DNA double strand break, providing a HDR template DNA construct comprising the amino acid marker corresponding to the second isoform of the surface protein and subsequently determining the expression of the first or second isoform of said surface protein on said cell, wherein expression of the second isoform indicates a successful HDR event. The invention also relates to a method for editing a genomic location of interest within a eukaryotic cell, and to a method of selectively depleting or enriching an edited cell in a composition of non-edited and edited cells.