公开(公告)号：US20220356445A1

公开(公告)日：2022-11-10

申请号：US17624584

申请日：2020-07-03

申请人： UNIVERSITE DE BORDEAUX ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE ,  UNIVERSITE DE ROUEN-NORMANDIE ,  INSTITUTE NATIONAL DE LA SANTE ET DE LA RECHERCE MEDICALE

发明人： MAXIME FEYEUX ,  KEVIN ALESSANDRI ,  MAGALIE LECOURTOIS ,  LAETITIA MIGUEL ,  PIERRE NASSOY ,  ANNE ROVELET LECRUX

IPC分类号： C12N5/0793

摘要： The present invention relates to a method for in-vitro production of mammalian neurons expressing the 6 isoforms of the Tau protein (2N4R, 1N4R, 0N4R, 2N3R, 1N3R, 0N3R), comprising a step of neuronal differentiation, in which cellular microcompartments are cultivated for a period of 5 weeks to 100 weeks, each one comprising a hollow hydro gel capsule surrounding post-mitotic neuronal cells and an extracellular matrix, the neuronal differentiation step being carried out in a bioreactor, the cellular microcompartments being kept in suspension in an enclosure of the bioreactor containing a neuronal differentiation medium.

公开(公告)号：US20230257699A1

公开(公告)日：2023-08-17

申请号：US18124921

申请日：2023-03-22

申请人： UNIVERSITE DE BORDEAUX ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE

发明人： MAXIME FEYEUX ,  KEVIN ALLESSANDRI ,  PIERRE NASSOY ,  LAURENT COGNET ,  ABDELHAMID BENAZZOUZ ,  ERWAN BEZARD

IPC分类号： C12N5/0793 ,  A61K35/30 ,  C12N5/00

CPC分类号： C12N5/0619 ,  A61K35/30 ,  C12N5/0012 ,  C12N2501/39 ,  C12N2501/42 ,  C12N2506/45

摘要： The invention relates to a neural tissue unit for use in implantation into the nervous system of a human or non-human mammal, wherein said neural tissue unit contains differentiated post-mitotic neuronal cells in an extracellular matrix, said unit being obtained from a cellular microcompartment comprising a hydrogel capsule surrounding the neural tissue unit, and said hydrogel capsule being at least partially removed before use of the neural tissue unit. The invention also relates to a process for preparing such a neural tissue unit.

公开(公告)号：US20200002681A1

公开(公告)日：2020-01-02

申请号：US16491662

申请日：2018-03-08

申请人： UNIVERSITE DE BORDEAUX ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE ,  INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE

发明人： LAETITIA ANDRIQUE ,  GAËLLE RECHER ,  KEVIN ALESSANDRI ,  MAXIME FEYEUX ,  PIERRE NASSOY ,  ANDRÉAS BIKFALVI

IPC分类号： C12N5/071 ,  C12N5/077

摘要： The invention relates to a hollow cell microfibre comprising successively, organized around a lumen, at least one endothelial cell layer, at least one smooth muscle cell layer, an extracellular matrix layer, and optionally an outer hydrogel layer. The invention also relates to a process for fabricating such a hollow cell microfibre.

公开(公告)号：US20230117999A1

公开(公告)日：2023-04-20

申请号：US18083912

申请日：2022-12-19

申请人： UNIVERSITE DE BORDEAUX ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE

发明人： MAXIME FEYEUX ,  KEVIN ALESSANDRI ,  PIERRE NASSOY ,  LAURENT COGNET ,  GAELLE RECHER ,  ERWAN BEZARD

IPC分类号： C12N5/00 ,  C12M1/00 ,  C12M1/12 ,  C12N5/074

摘要： The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.

公开(公告)号：US20200370022A1

公开(公告)日：2020-11-26

申请号：US16603264

申请日：2018-04-05

申请人： UNIVERSITE DE BORDEAUX ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) ,  INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE ,  INSTITUT BERGONIE ,  UNIVERSITE DE RENNES 1 ,  ETABLISSEMENT FRANCAIS DU SANG ,  CENTRE HOSPITALIER UNIVERSITAIRE (CHU) DE RENNES

发明人： LAURENCE BRESSON-BEPOLDIN ,  SIMON LATOUR ,  ISABELLE MAHOUCHE ,  PIERRE NASSOY ,  KEVIN ALESSANDRI ,  KARIN TARTE ,  FRÉDÉRIC MOURCIN

IPC分类号： C12N5/09 ,  C12N5/00 ,  C12N5/0781 ,  G01N33/50

摘要： The invention relates to a process for preparing cellular microcompartments comprising a hydrogel capsule surrounding a cluster of lymphomatous cells. The invention also relates to such a cellular microcompartment and the use thereof for screening anti-cancer molecules.

公开(公告)号：US20190330589A1

公开(公告)日：2019-10-31

申请号：US16462962

申请日：2017-11-23

申请人： UNIVERSITE DE BORDEAUX ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE

发明人： MAXIME FEYEUX ,  KEVIN ALESSANDRI ,  PIERRE NASSOY ,  LAURENT COGNET ,  GAËLLE RECHER ,  ERWAN BEZARD

IPC分类号： C12N5/00 ,  C12N5/074 ,  C12M1/00 ,  C12M1/12

摘要： The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.

公开(公告)号：US20210123013A1

公开(公告)日：2021-04-29

申请号：US17057143

申请日：2019-05-20

申请人： UNIVERSITE DE BORDEAUX ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT D'OPTIQUE THÉORIQUE ET APPLIQUÉE

发明人： MAXIME FEYEUX ,  KEVIN ALESSANDRI ,  PIERRE NASSOY

IPC分类号： C12N5/00 ,  C12M1/32 ,  C12M1/00 ,  C12N1/04

摘要： The invention relates to a bioreactor cell culture system comprising a closed chamber containing a plurality of suspended cell microcompartments, wherein the microcompartments each comprise an outer hydrogel layer providing a cavity containing a set of self-organized cells and extracellular matrix or an extracellular matrix substitute. The invention further relates to the use of such bioreactors to produce cells and/or organoids, and/or molecules and/or complex molecular assemblies.

公开(公告)号：US20200063099A1

公开(公告)日：2020-02-27

申请号：US16462965

申请日：2017-11-23

申请人： UNIVERSITÉ DE BORDEAUX ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT D'OPTIQUE GRADUATE SCHOOL

发明人： MAXIME FEYEUX ,  KEVIN ALESSANDRI ,  PIERRE NASSOY ,  LAURENT COGNET ,  ABDELHAMID BENAZZOUZ ,  ERWAN BEZARD

IPC分类号： C12N5/0793 ,  C12N5/00 ,  A61K35/30

摘要： The invention relates to a neural tissue unit for use in implantation into the nervous system of a human or non-human mammal, wherein said neural tissue unit contains differentiated post-mitotic neuronal cells in an extracellular matrix, said unit being obtained from a cellular microcompartment comprising a hydrogel capsule surrounding the neural tissue unit, and said hydrogel capsule being at least partially removed before use of the neural tissue unit. The invention also relates to a process for preparing such a neural tissue unit.