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公开(公告)号：US10590452B2
公开(公告)日：2020-03-17
申请号：US15849006
申请日：2017-12-20
Inventor： Jong-Bum Lee , Jae-Sung Lee
Abstract: This invention relates to DNA-Mn hybrid particles and a method of manufacturing the same, the method including producing a circular DNA template for replication and forming particles in which DNA and Mn are bound to each other using Mn during the synthesis of a new strand of DNA from the circular DNA template for replication using a DNA polymerase, thus promoting the activity of the DNA polymerase using the coenzyme function of Mn and broadening the range of application fields of DNA as a biomaterial.
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公开(公告)号：US10300148B2
公开(公告)日：2019-05-28
申请号：US15514889
申请日：2015-10-01
Inventor： Jong-Bum Lee , Hye-Jin Kim
Abstract: Disclosed are nanoparticles that are introduced into cells and express a specific protein and a manufacturing method thereof. More particularly, the present invention relates to mRNA nanoparticles, which increase the expression of a specific protein capable of stimulating the cellular immune system to induce cellular immune responses and are thus applicable to treat a variety of diseases, do not require passage across the nuclear envelope because a desired gene is delivered not as plasmid DNA itself but in the form of mRNA, thus improving the efficiency of protein expression, and the nanoparticles are generated through a one-step process with a relatively small amount of plasmid DNA via rolling circle transcription (RCT), thereby providing a simple and economical process for gene delivery. The present invention is also concerned with such mRNA nanoparticles.
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公开(公告)号：US10253317B2
公开(公告)日：2019-04-09
申请号：US15321748
申请日：2015-06-30
Inventor： Jong-Bum Lee , Yong-Kuk Park
IPC: C12N15/113 , C12N15/64 , C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q1/6867
Abstract: Disclosed are particles which are introduced into target cells and suppress the expression of specific genes, and a method of manufacturing such particles. More particularly, the present invention relates to DNA-RNA hybrid particles that comprise a DNA strand and an RNA strand that binds to the DNA strand through partial complementary base pairing, in which the DNA strand comprises an aptamer sequence that is able to bind to a target protein produced in a target cell, and the RNA strand comprises an siRNA sequence that binds to a target RNA in the target cell to suppress protein expression from the target RNA. Such hybrid particles are capable of effectively delivering an siRNA therapeutic agent into target cells for the treatment of disease, and have resistance against digestion by in vivo nucleases, DNase and RNase, owing to complementary binding formed between DNA and RNA strands. Also, the present invention relates to a method of manufacturing such DNA-RNA hybrid particles.
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公开(公告)号：US09663809B2
公开(公告)日：2017-05-30
申请号：US15035747
申请日：2014-11-18
Inventor： Jong-Bum Lee , Dae-Hoon Han
CPC classification number: C12P19/34 , C12N15/10 , C12N15/1003
Abstract: Disclosed are a method of manufacturing an RNA membrane and an RNA membrane manufactured thereby, wherein the RNA membrane can be produced at lower cost, in which the RNA membrane is composed exclusively of RNA, and thus has no toxicity in vivo, is controllable, and can be effectively applied to bio-organs such as the pericardium, as well as the production of peptides or proteins, and particularly, long linear RNA strands, which have not yet formed particles, are concentrated on the surface of a tube by inducing an evaporation process to thus activate the bonding of base pairs thereof, and the roughness and thickness of the RNA membrane can be controlled by changing the manufacturing conditions.
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