公开(公告)号：US20230272462A1

公开(公告)日：2023-08-31

申请号：US18004489

申请日：2021-07-09

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Enos Kline ,  Michael Roller ,  Robert G. Atkinson ,  Nuttada Panpradist ,  Daniel Leon ,  Ian Hull ,  Qin Wang ,  Barry R. Lutz

IPC: C12Q1/6844 ,  C12Q1/6883 ,  C12N15/63 ,  B01L7/00

CPC classification number: C12Q1/6844 ,  C12Q1/6883 ,  C12N15/63 ,  B01L7/52 ,  C12N2310/122 ,  B01L2300/1822

Abstract: Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid in a sample are described. In an embodiment, the kit comprises a loop primer nucleic acid molecule configured for loop-mediated isothermal amplification (LAMP), the loop primer nucleic acid molecule comprising: a targeting sequence complementary to a target portion of a target nucleic acid sequence; and an adapter sequence; a displacement nucleic acid probe comprising: a fluorophore adapter sequence; and the adapter sequence; and a fluorophore adapter complement nucleic acid molecule complementary to the fluorophore adapter sequence, wherein the fluorophore adapter sequence or the fluorophore adapter complement nucleic acid molecule is coupled to a fluorophore. In an embodiment, the system comprises a thermal subsystem for heating a sample disposed therein, and an optical subsystem for optically excited the sample and detecting light emitted from the sample.