公开(公告)号：US20230272462A1

公开(公告)日：2023-08-31

申请号：US18004489

申请日：2021-07-09

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Enos Kline ,  Michael Roller ,  Robert G. Atkinson ,  Nuttada Panpradist ,  Daniel Leon ,  Ian Hull ,  Qin Wang ,  Barry R. Lutz

IPC: C12Q1/6844 ,  C12Q1/6883 ,  C12N15/63 ,  B01L7/00

CPC classification number: C12Q1/6844 ,  C12Q1/6883 ,  C12N15/63 ,  B01L7/52 ,  C12N2310/122 ,  B01L2300/1822

Abstract: Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid in a sample are described. In an embodiment, the kit comprises a loop primer nucleic acid molecule configured for loop-mediated isothermal amplification (LAMP), the loop primer nucleic acid molecule comprising: a targeting sequence complementary to a target portion of a target nucleic acid sequence; and an adapter sequence; a displacement nucleic acid probe comprising: a fluorophore adapter sequence; and the adapter sequence; and a fluorophore adapter complement nucleic acid molecule complementary to the fluorophore adapter sequence, wherein the fluorophore adapter sequence or the fluorophore adapter complement nucleic acid molecule is coupled to a fluorophore. In an embodiment, the system comprises a thermal subsystem for heating a sample disposed therein, and an optical subsystem for optically excited the sample and detecting light emitted from the sample.

公开(公告)号：US20230134587A1

公开(公告)日：2023-05-04

申请号：US17888335

申请日：2022-08-15

Applicant: University of Washington

Inventor： Sarah J. Iribarren ,  Barry R. Lutz ,  Daniel Leon ,  Marcus Rhodehamel ,  Jesse Keyes ,  Hannah Erdy ,  Mohamed Adil ,  Rishika Das ,  Kyle Goodwin

IPC: G01N33/50 ,  G01N33/493 ,  G16H20/10 ,  G01N21/01

Abstract: Embodiments disclosed herein relate to apparatuses, systems, and methods for detecting biomarkers in biological samples present in assay tests using a control strip and indicator strip, where the control strip includes reagents that form a reactive gas which reacts with the biomarker on the indicator strip, if present. Methods of using the test apparatuses include taking a sending photographs of a used test apparatus to a medical provider to demonstrate adherence to a pharmaceutical treatment regimen.