公开(公告)号：US11648560B2

公开(公告)日：2023-05-16

申请号：US16639083

申请日：2018-08-15

Applicant: UNIVERSITY OF WASHINGTON ,  MICAREO INC.

Inventor： Daniel T. Chiu ,  Mengxia Zhao ,  Eleanor S. Shah ,  Perry G. Schiro ,  Hui Min Yu ,  Wei-Feng Fang ,  Jui-Lin Chen

IPC: B01L3/00 ,  G01N15/14 ,  G01N33/49 ,  G01N15/10

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/502738 ,  G01N15/1434 ,  G01N15/1459 ,  G01N33/4915 ,  B01L2200/0663 ,  B01L2300/06 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2400/06 ,  B01L2400/084 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2015/1486

Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and can be contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.