公开(公告)号：US20220260550A1

公开(公告)日：2022-08-18

申请号：US17627730

申请日：2020-07-15

申请人： Universal Sequencing Technology Corporation

发明人： Peiming Zhang ,  Ming Lei ,  Kisup Chung ,  Xinyue Zhang ,  Barrett Duan

IPC分类号： G01N33/487 ,  G01N27/447 ,  G01N27/327

摘要： The present invention relates to a nanopore device with a motion control mechanism to control the speed of a polymeric molecule translocating through the nanopore for a tunneling nanogap to read out its sequences or components.

公开(公告)号：US20220251638A1

公开(公告)日：2022-08-11

申请号：US17595758

申请日：2020-05-27

申请人： Universal Sequencing Technology Corporation

发明人： Peiming Zhang ,  Ming Lei

IPC分类号： C12Q1/6869

摘要： This invention provides methods to identify or sequence a DNA or RNA molecule electronically in a single molecule level based on polymerase synthesis.

公开(公告)号：US20240218415A1

公开(公告)日：2024-07-04

申请号：US17907111

申请日：2021-03-25

申请人： Universal Sequencing Technology Corporation

发明人： Peiming Zhang ,  Sanjay B. Hari ,  Kisup Chung ,  Ming Lei

IPC分类号： C12Q1/00

CPC分类号： C12Q1/001

摘要： This invention is related to a nanogap device for the electronic sensing of biomolecules.

公开(公告)号：US20240192204A1

公开(公告)日：2024-06-13

申请号：US17998556

申请日：2021-05-17

申请人： Universal Sequencing Technology Corporation

发明人： Peiming Zhang ,  Ming Lei ,  Sanjay B. Hari

IPC分类号： G01N33/543 ,  G01N33/553 ,  G01N33/569

CPC分类号： G01N33/54388 ,  G01N33/54346 ,  G01N33/553 ,  G01N33/56983 ,  G01N2333/165

摘要： This invention provides a device and assays for the detection of pathogens.

公开(公告)号：US20230220453A1

公开(公告)日：2023-07-13

申请号：US18149397

申请日：2023-01-03

申请人： Universal Sequencing Technology Corporation

发明人： Zhoutao Chen ,  Long Kim Pham ,  Junchen Gu ,  Ming Lei

IPC分类号： C12Q1/6834 ,  C12Q1/6874 ,  C40B40/06 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12N15/10

CPC分类号： C12Q1/6834 ,  C12Q1/6874 ,  C40B40/06 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12N15/1065 ,  C12Q2563/185

摘要： The present disclosure provides methods and kits for tracking nucleic acid target origin by barcode tagging of the targets when they break into smaller fragments. Nucleic acid targets are captured in vitro by clonally localized nucleic acid barcode templates on a solid support. Millions of nucleic acid targets can be processed simultaneously in a massively parallel fashion without additional partition. These captured targets are broken into small fragments, and a target specific barcode sequence is tagged on each fragment as an identification of their original target. These nucleic acid target tracking methods can be used for a variety of applications in both whole genome sequencing and targeted sequencing.

公开(公告)号：US20220186294A1

公开(公告)日：2022-06-16

申请号：US17604046

申请日：2020-04-15

申请人： Universal Sequencing Technology Corporation

发明人： Peiming Zhang ,  Ming Lei ,  Kisup Chung

IPC分类号： C12Q1/6809 ,  C12Q1/6869

摘要： This invention provides a device for sequencing and identification of biopolymers electronically.

公开(公告)号：US20180298436A1

公开(公告)日：2018-10-18

申请号：US15767428

申请日：2016-10-31

申请人： UNIVERSAL SEQUENCING TECHNOLOGY CORPORATION

发明人： Ming Lei ,  Robert Roorda ,  Zhoutao Chen ,  Nga Ho ,  Yong Wang

IPC分类号： C12Q1/6869 ,  G01N33/487

摘要： The present disclosure provides, in one aspect, a device and a method for unit sequencing and/or analysis of a molecular sequence comprising attaching the molecular sequence to a plate and controlling the progression of the molecular sequence through a pore of a nanopore chip, wherein the separation distance between the nanopore chip and the scan plate is controlled by a precision mechanical drive, and the molecular sequence is sensed as it progresses through the nanopore.

公开(公告)号：US11977070B2

公开(公告)日：2024-05-07

申请号：US16613016

申请日：2018-05-11

申请人： UNIVERSAL SEQUENCING TECHNOLOGY CORPORATION DBA UST CORPORATION

发明人： Robert Roorda ,  Ming Lei ,  Nga Ho

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  G01N33/487

CPC分类号： G01N33/48721 ,  C12Q1/6869

摘要： The present disclosure relates generally to methods of controlling the movement of molecules, such as DNA (Deoxyribonucleic Acid), RNA (Ribonucleic Acid) and proteins as well as other biological molecules passing through nanopores. In particular, the disclosure describes how structures made of soft magnetic material can be used to selectively hold, pull and release DNA tagged with magnetic beads.

公开(公告)号：US20190078150A1

公开(公告)日：2019-03-14

申请号：US16077295

申请日：2017-03-01

申请人： Universal Sequencing Technology Corporation

发明人： Zhoutao Chen ,  Long Kim Pham ,  Junchen Gu ,  Ming Lei

IPC分类号： C12Q1/6834 ,  C12Q1/6874 ,  C12Q1/6876 ,  C40B40/06

摘要： The present disclosure provides methods and kits for tracking nucleic acid target origin by barcode tagging of the targets when they break into smaller fragments. Nucleic acid targets are captured in vitro by clonally localized nucleic acid barcode templates on a solid support. Millions of nucleic acid targets can be processed simultaneously in a massively parallel fashion without additional partition. These captured targets are broken into small fragments, and a target specific barcode sequence is tagged on each fragment as an identification of their original target. These nucleic acid target tracking methods can be used for a variety of applications in both whole genome sequencing and targeted sequencing.

公开(公告)号：US20230070226A1

公开(公告)日：2023-03-09

申请号：US17759742

申请日：2021-01-31

申请人： Universal Sequencing Technology Corporation

发明人： Sanjay B. Hari ,  Peiming Zhang ,  Barrett Duan ,  Ming Lei

IPC分类号： C12Q1/6825 ,  C12Q1/6869 ,  G01N33/487 ,  G01N33/543 ,  G16B30/10 ,  C12Q1/6876

摘要： The present invention provides methods to engineer enzymes for their integration into a molecular nanowire as a fum-tional component for biopolymer sequencing/identification. The enzymes include but are not limited to DNA polymerase, RNA poly-merase, DNA helicase, DNA ligase, DNA exonuclease, reverse transcriptase, RNA primase, ribosome, sucrase, or lactase, which are either natural, mutated, or synthesized.