公开(公告)号：US20240019398A1

公开(公告)日：2024-01-18

申请号：US18372558

申请日：2023-09-25

申请人： UNIVERSITY OF HOUSTON SYSTEM

发明人： Dmitri Litvinov ,  Long Chang ,  Richard Willson

IPC分类号： G01N27/74 ,  G01N33/543

CPC分类号： G01N27/745 ,  G01N33/54326 ,  G01N33/54346 ,  G01N33/54373

摘要： A biosensor may provide a magnetoresistive (MR) film comprising a nonmagnetic layer may be sandwiched between the two ferromagnetic layers. The MR film may be positioned on a substrate, where the edges of the MR film are in contact with leads. The leads may be in contact with pads. The sensors may provide quasi-digital readout that enable greatly enhanced sensitivity. In some embodiments, biosensors may be arranged as array of sensors. The array of sensors may be arranged as a symmetric or asymmetric N1×N2 array, where N1 and N2 are integers, N1 represents the number of sensors linked together in series, and N2 represents the number of sensor sets in parallel, where each sensor set may comprise one or more sensors. Further, the array of sensors may be coupled to a voltmeter, which may be a single voltmeter in that allows the sensors to all be probed simultaneously.

公开(公告)号：US11821818B2

公开(公告)日：2023-11-21

申请号：US16624643

申请日：2018-07-06

申请人： University of Houston System

发明人： Dmitri Litvinov ,  Long Chang ,  Richard Willson

IPC分类号： G01N27/74 ,  G01N33/543

CPC分类号： G01N27/745 ,  G01N33/54326 ,  G01N33/54346 ,  G01N33/54373

摘要： A biosensor may provide a magnetoresistive (MR) film comprising a nonmagnetic layer may be sandwiched between the two ferromagnetic layers. The MR film may be positioned on a substrate, where the edges of the MR film are in contact with leads. Additionally, the leads may be in contact with pads. The sensors may provide quasi-digital readout that enable greatly enhanced sensitivity. In some embodiments, biosensors may be arranged as array of sensors. The array of sensors may be arranged as a symmetric or asymmetric N1×N2 array, where N1 and N2 are integers, N1 represents the number of sensors linked together in series, and N2 represents the number of sensor sets in parallel, where each sensor set may comprise one or more sensors. Further, the array of sensors may be coupled to a voltmeter, which may be a single voltmeter in some cases that allows the sensors to all be probed simultaneously.

公开(公告)号：US10961564B2

公开(公告)日：2021-03-30

申请号：US15314219

申请日：2015-05-22

申请人： UNIVERSITY OF HOUSTON SYSTEM

发明人： Wei-Chuan Shih ,  Richard Willson

IPC分类号： C12Q1/6816 ,  G01N21/65 ,  G01N21/64 ,  G01N21/552 ,  G01N33/543 ,  G01N33/553

摘要： A methodology for assays and diagnostics utilizes a nanoporous or corrugated metal-containing surface, fiber or particle which enhances or suppresses the optical detectability of a label. The resulting optical, electromagnetic, or imaging signal signals the presence of a pathogen or analyte of interest. Preferred embodiments pertain to label-free, in situ monitoring of individual DNA hybridization in microfluidics using molecular sentinel probes immobilized on nanoporous gold disks. By immobilizing molecular sentinel probes on nanoporous gold disks, single-molecule sensitivity is demonstrated via surface-enhanced Raman scattering which provides robust signals. The described methodology is generally applicable to most amplification independent assays and molecular diagnostics.

公开(公告)号：US09733315B2

公开(公告)日：2017-08-15

申请号：US13762622

申请日：2013-02-08

申请人： University of Houston

发明人： Dmitri Litvinov ,  Richard Willson

IPC分类号： G01R33/00 ,  G01R33/12

CPC分类号： G01R33/00 ,  G01N2446/00 ,  G01R33/1269

摘要： A biomolecular sensor system includes an array of magnetoresistive nanosensors designed for sensing biomolecule-conjugated superparamagnetic nanoparticles. Materials and geometry of each sensor element are designed for optimized sensitivity. The system includes magnetic field generators to apply forces to superparamagnetic nanoparticles for 1) nanoparticle manipulation, 2) sensor magnetic biasing, 3) magnetic pull-off measurement for differentiation against non-specific association, and 4) removal of all particles from the sensor array surface.

公开(公告)号：US10948411B2

公开(公告)日：2021-03-16

申请号：US15711102

申请日：2017-09-21

申请人： University of Houston System

发明人： Jakoah Brgoch ,  Erin Finley ,  Andrew Paterson ,  Richard Willson

IPC分类号： G01N21/64 ,  C09K11/77 ,  G01N33/58 ,  G01N33/543 ,  G01N21/84

摘要： Disclosed are methods of detecting one or more analytes in a sample by: (1) associating the sample with a surface that includes an analyte binding agent to result in the immobilization of the analytes on the surface; (2) contacting the analyte with a composition that includes at least one phosphor compound with an affinity for the analyte; (3) formation of immobilized analyte binding agent-analyte-phosphor complexes on the surface; (4) separating unbound phosphor compounds from the immobilized complexes; (5) detecting a presence or absence of a luminescence signal from the immobilized complexes; and (6) correlating the luminescence signal to the presence or absence of the analyte in the sample. The phosphor compound may include (Sr1-δBaδ)2-j-kMgSi2O7:EujDyk, (Sr1-δBaδ)2-xMgSi2O7:Eu2+Dy3+, (Sr1-δBaδ)2MgSi2O7:Eu2+Dy3+, (Sr1-δBaδ)2-xMgSi2O7:Eu2+, and combinations thereof. Additional phosphor compounds may also be utilized, such as [AE]2MgSi2O7:Eu2+, [AE]Al2O4:Eu2+, Dy3+, and combinations thereof, where AE is at least one of Ca, Sr, or Ba.

公开(公告)号：US10533996B2

公开(公告)日：2020-01-14

申请号：US14461118

申请日：2014-08-15

申请人： University of Houston

发明人： Richard Willson ,  Andrew Paterson

IPC分类号： C12Q1/68 ,  G01N33/569

摘要： In some embodiments, the present disclosure pertains to new compositions of matter that comprise phosphorescent reporters. In some embodiments, the phosphorescent reporters of the present disclosure comprise strontium aluminate. In some embodiments, the strontium aluminate is doped with europium and dysprosium (SrAl2O4:Eu2+, Dy3+). Additional embodiments of the present disclosure pertain to methods of making the aforementioned phosphorescent reporters. In some embodiments, the method includes size reduction of inorganic phosphorescent powders through a combination of wet milling and settling. In additional embodiments, the present disclosure pertains to methods of detecting the phosphorescent reporters in various settings, such as diagnostic settings.