公开(公告)号：US11884926B2

公开(公告)日：2024-01-30

申请号：US16966504

申请日：2019-02-01

Applicant: University of Massachusetts

Inventor： Terence Flotte ,  Qiushi Tang ,  Allison Keeler-Klunk ,  Qin Su

IPC: C12N15/70 ,  C12N7/00

CPC classification number: C12N15/70 ,  C12N7/00 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2750/14171

Abstract: In some aspects, the disclosure relates to compositions and methods for production of recombinant adeno-associated virus (rAAV) particles. The disclosure is based, in part, on isolated nucleic acids and systems that include only two vectors and are suitable for production of multiple different single gene viral vectors in a multiple small-scale campaign mode (e.g., 1013 to 1016 viral particles.)

公开(公告)号：US20210047650A1

公开(公告)日：2021-02-18

申请号：US16966504

申请日：2019-02-01

Applicant: University of Massachusetts

Inventor： Terence Flotte ,  Qiushi Tang ,  Allison Keeler-Klunk ,  Qin Su

IPC: C12N15/70 ,  C12N7/00

Abstract: In some aspects, the disclosure relates to compositions and methods for production of recombinant adeno-associated virus (rAAV) particles. The disclosure is based, in part, on isolated nucleic acids and systems that include only two vectors and are suitable for production of multiple different single gene viral vectors in a multiple small-scale campaign mode (e.g., 1013 to 1016 viral particles.)

公开(公告)号：US20240216534A1

公开(公告)日：2024-07-04

申请号：US18288227

申请日：2022-04-26

Applicant: University of Massachusetts

Inventor： Terence Flotte ,  Allison Keeler-Klunk

IPC: A61K48/00 ,  A61K38/42 ,  A61P7/00 ,  C07K14/005 ,  C12N9/20 ,  C12N15/11 ,  C12N15/86

CPC classification number: A61K48/005 ,  A61K38/42 ,  A61P7/00 ,  C07K14/005 ,  C12N9/20 ,  C12N15/11 ,  C12N15/86 ,  C12N2310/20 ,  C12N2750/14122 ,  C12N2750/14143

Abstract: Aspects of the disclosure relate to compositions and methods for gene editing in a cell or subject. In some aspect, the present disclosure provides an isolated nucleic acid comprising an expression construct comprising transgene encoding a gene product flanked by a 5′ homology arm and a 3′ homology arm, wherein the expression construct is flanked by adeno-associated virus (AAV) inverted terminal repeats (ITRs).

公开(公告)号：US20220220170A1

公开(公告)日：2022-07-14

申请号：US17605615

申请日：2020-04-23

Applicant: University of Massachusetts

Inventor： Allison Keeler-Klunk ,  Terence Flotte ,  Motahareh Arjomandnejad

IPC: C07K14/47 ,  C07K14/005 ,  C07K14/705 ,  C07K14/725 ,  C12N15/62 ,  C12N15/86

Abstract: Aspects of the disclosure relate to compositions and methods for modulating the immune response of a subject (e.g., a human subject) to certain viral antigens (e.g., antigens derived from AAV capsid proteins). The disclosure is based, in part, on isolated nucleic acids and expression constructs encoding chimeric antigen receptors (CARs) that target AAV capsid proteins, and recombinant immune cells comprising such constructs (e.g., recombinant T-cell comprising a CAR (CAR T-cells), and recombinant T-regulatory cells comprising a CAR (CAR T-regs)).