公开(公告)号：US20240158846A1

公开(公告)日：2024-05-16

申请号：US18486103

申请日：2023-10-12

Applicant: University of Washington through its Center for Commercialization

Inventor： Jens Gundlach ,  Ian M. Derrington ,  Andrew Laszlo ,  Elizabeth Manrao

IPC: C12Q1/6869 ,  G01N27/447 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  G01N27/447 ,  G01N33/48721

Abstract: The present disclosure generally relates to the methods and compositions to efficiently analyze polymer characteristics using nanopore-based assays. Specifically disclosed is a method for generating reference signals for polymer analysis in a nanopore system, wherein the nanopore system has a multi-subunit output signal resolution. The method comprises translocating a reference sequence through a nanopore to generate a plurality of reference output signals, wherein each possible multi-subunit sequence that can determine an output signal appears only once in the reference sequence. The output signals are compiled into a reference map for nanopore analysis of an analyte polymer. Also provided are methods and compositions for calibrating the nanopore system for optimized polymer analysis.

公开(公告)号：US10822652B2

公开(公告)日：2020-11-03

申请号：US16029396

申请日：2018-07-06

Applicant: University of Washington through its Center for Commercialization ,  Illumina, Inc.

Inventor： Jens H. Gundlach ,  Andrew Laszlo ,  Ian Derrington ,  Jeffrey G. Mandell

IPC: C12Q1/68 ,  C12Q1/6869 ,  B01D57/02 ,  G01N33/487

Abstract: The present disclosure provides method and systems for improving nanopore-based analysis of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind in a re-analyte polymer that results in a polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

公开(公告)号：US20240294980A1

公开(公告)日：2024-09-05

申请号：US18486061

申请日：2023-10-12

Applicant: University of Washington through its Center for Commercialization

Inventor： Jens H. Gundlach ,  Andrew Laszlo

IPC: C12Q1/6869

CPC classification number: C12Q1/6869

Abstract: The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.

公开(公告)号：US11959133B2

公开(公告)日：2024-04-16

申请号：US16824186

申请日：2020-03-19

Applicant: University of Washington through its Center for Commercialization

Inventor： Jens Gundlach ,  Ian M. Derrington ,  Andrew Laszlo ,  Elizabeth Manrao

IPC: C12Q1/6869 ,  G01N27/447 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  G01N27/447 ,  G01N33/48721

Abstract: The present disclosure generally relates to the methods and compositions to efficiently analyze polymer characteristics using nanopore-based assays. Specifically disclosed is a method for generating reference signals for polymer analysis in a nanopore system, wherein the nanopore system has a multi-subunit output signal resolution. The method comprises translocating a reference sequence through a nanopore to generate a plurality of reference output signals, wherein each possible multi-subunit sequence that can determine an output signal appears only once in the reference sequence. The output signals are compiled into a reference map for nanopore analysis of an analyte polymer. Also provided are methods and compositions for calibrating the nanopore system for optimized polymer analysis.

公开(公告)号：US20210172013A1

公开(公告)日：2021-06-10

申请号：US17111167

申请日：2020-12-03

Applicant: University of Washington through its Center for Commercialization

Inventor： Jens H. Gundlach ,  Andrew Laszlo

IPC: C12Q1/6869

Abstract: The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.

公开(公告)号：US20160222444A1

公开(公告)日：2016-08-04

申请号：US14915611

申请日：2014-09-02

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION ,  ILLUMINA, INC.

Inventor： Jens H. Gundlach ,  Andrew Laszlo ,  Ian Derrington ,  Jeffrey G. Mandell

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01D57/02 ,  G01N33/48721 ,  C12Q2521/101 ,  C12Q2523/303 ,  C12Q2525/107 ,  C12Q2525/113 ,  C12Q2525/119 ,  C12Q2565/631 ,  C12Q2523/125

Abstract: The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

Abstract translation: 本公开提供了用于改进基于纳米孔的聚合物分析的方法和系统。 本公开提供了用于选择性地修饰一种或多种单分子的方法，所述单体亚单位使得分析物聚合物与修饰的亚基形成聚合物分析物。 聚合物分析物在基于纳米孔的系统中产生可检测的信号。 可检测信号和/或其与参考信号的偏差指示经修饰的亚基在聚合物分析物中的位置，因此允许在原始预分析物聚合物的该位置识别亚基。

公开(公告)号：US20150191782A1

公开(公告)日：2015-07-09

申请号：US14419217

申请日：2013-08-02

Applicant: University of Washington through its Center for Commercialization

Inventor： Jens H. Gundlach ,  Andrew Laszlo

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q2523/31 ,  C12Q2565/631

Abstract: The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.

Abstract translation: 本公开提供了用于改进聚合物的基于纳米孔的分析的方法和试剂。 具体地，本公开提供了一种分析包含聚合物分析物的聚合物的方法，所述聚合物分析物含有具有至少一个带电部分的末端结构域。 本公开还提供了增加聚合物分析物和纳米孔之间的相互作用速率的方法，其中聚合物分析物含有具有至少一个带电部分的末端结构域。 本公开还提供了与所述方法一起使用的组合物，包括含有带电部分的接合体组合物，例如磷酸盐或硫酸盐基团，并且被配置为连接到聚合物分析物结构域。

公开(公告)号：US11821033B2

公开(公告)日：2023-11-21

申请号：US17111167

申请日：2020-12-03

Applicant: University of Washington through its Center for Commercialization

Inventor： Jens H. Gundlach ,  Andrew Laszlo

IPC: C12Q1/6869

CPC classification number: C12Q1/6869 ,  C12Q1/6869 ,  C12Q2523/31 ,  C12Q2565/631

Abstract: The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.

公开(公告)号：US20210032695A1

公开(公告)日：2021-02-04

申请号：US17028784

申请日：2020-09-22

Applicant: University of Washington through its Center for Commercialization ,  Illumina, Inc.

Inventor： Jens H. Gundlach ,  Andrew Laszlo ,  Ian Derrington ,  Jeffrey G. Mandell

IPC: C12Q1/6869 ,  B01D57/02

Abstract: The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

公开(公告)号：US20190106740A1

公开(公告)日：2019-04-11

申请号：US16029396

申请日：2018-07-06

Applicant: University of Washington through its Center for Commercialization ,  Illumina, Inc.

Inventor： Jens H. Gundlach ,  Andrew Laszlo ,  Ian Derrington ,  Jeffrey G. Mandell

IPC: C12Q1/6869

Abstract: The present disclosure provides method and systems for improving nanopore-based analysis of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind in a re-analyte polymer that results in a polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.