公开(公告)号：US20170198286A1

公开(公告)日：2017-07-13

申请号：US15448818

申请日：2017-03-03

申请人： VILNIUS UNIVERSITY

发明人： Virginijus Siksnys ,  Migle Kazlauskiene ,  Gintautas Tamulaitis

IPC分类号： C12N15/11 ,  C12N9/22 ,  C12N15/113

CPC分类号： C12N15/111 ,  C07K14/315 ,  C12N9/22 ,  C12N15/113 ,  C12N2310/10 ,  C12N2310/20 ,  C12Y301/27

摘要： A Type III-A CRISPR-Cas (StCsm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.

公开(公告)号：US10385336B2

公开(公告)日：2019-08-20

申请号：US15448818

申请日：2017-03-03

申请人： VILNIUS UNIVERSITY

发明人： Virginijus Siksnys ,  Migle Kazlauskiene ,  Gintautas Tamulaitis

IPC分类号： C12N15/11 ,  C12N15/113 ,  C12N9/22 ,  C07K14/315

摘要： A Type III-A CRISPR-Cas (StCsm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.

公开(公告)号：US12123031B2

公开(公告)日：2024-10-22

申请号：US16618238

申请日：2018-05-31

申请人： VILNIUS UNIVERSITY

发明人： Virginijus Šikšnys ,  Migle Kazlauskiene ,  Georgij Kostiuk ,  Gintautas Tamulaitis

IPC分类号： C12N9/22 ,  C12N9/12 ,  C12P19/34 ,  C12Q1/6811

CPC分类号： C12N9/22 ,  C12N9/1241 ,  C12P19/34 ,  C12Q1/6811 ,  C12Y207/07 ,  C12N2310/20

摘要： A method of synthetizing cyclic oligoadenylates using a novel catalytic activity of a protein possessing the Palm domain, such as the Cas10 protein, and using such compounds for activation of proteins possessing the CARF doman, such as the Csm6 protein.