公开(公告)号：US20060035297A1

公开(公告)日：2006-02-16

申请号：US11201992

申请日：2005-08-11

申请人： Vani Bodepudi ,  Amy Tsai ,  Weixing Luo ,  Rueyming Loor

发明人： Vani Bodepudi ,  Amy Tsai ,  Weixing Luo ,  Rueyming Loor

IPC分类号： G01N33/53 ,  C07K16/18 ,  C07K14/47

CPC分类号： G01N33/9493 ,  C07K16/44

摘要： The invention teaches derivatives of mycophenolic alcohol and methods of preparing immunogens and other conjugates useful in immunoassays for quantitatively measuring concentrations of mycophenolic acid (MPA) and/or active metabolites of MPA in patient specimens. Antibodies produced from the disclosed immunogens capable of binding to MPA with cross-reactivity of no more than 5% with inactive metabolites and commonly co-prescribed drugs. Further, immunoassays for measuring the concentration of MPA using such antibodies are taught.

摘要翻译： 本发明教导霉酚醇的衍生物和制备用于免疫测定的免疫原和其它缀合物的方法，用于定量测量患者标本中的MPA和/或MPA的活性代谢物的浓度。 从所公开的能够与MPA结合的免疫原产生的抗体产生的交联反应性不超过5％，与无活性代谢物和通常共同处方的药物。 此外，教导了使用这种抗体测量MPA浓度的免疫测定。

公开(公告)号：US20050176080A1

公开(公告)日：2005-08-11

申请号：US11049547

申请日：2005-02-02

申请人： Vani Bodepudi ,  Lisa Charter ,  Rueyming Loor

发明人： Vani Bodepudi ,  Lisa Charter ,  Rueyming Loor

IPC分类号： C07K14/47 ,  C07K16/14 ,  C07K16/18 ,  G01N33/53 ,  G01N33/535 ,  G01N33/537 ,  G01N33/543 ,  G01N33/94

CPC分类号： C07K16/14 ,  G01N33/535 ,  G01N33/9446

摘要： The invention teaches derivatives of ascomycin and methods of preparing immunogens and other conjugates useful in immunoassays for quantitatively measuring concentrations of tacrolimus in patient specimens. Antibodies produced from the disclosed immunogens capable of binding to tacrolimus with cross-reactivity of no more than 5% with each of 15-O-demethyl tacrolimus, 31-O-demethyl tacrolimus, and 13,31-O-didemethyl tacrolimus, less than 40% with 13-O-demethyl tacrolimus, and less than 1% with cyclosporin, rapamycin, mycophenolic acid, prednisone, hydrocortisol, and prednisolone are described. Further, immunoassays for measuring the concentration of tacrolimus using such antibodies are taught.

摘要翻译： 本发明教导了子囊霉的衍生物以及制备用于免疫测定的免疫原和其它缀合物的方法，用于定量测量患者标本中他克莫司的浓度。 从所公开的能够与15- O-去甲基他克莫司，31-O-去甲基他克莫司和13,31-O-二甲基他克莫司结合的他克莫司的交叉反应性不超过5％的免疫原产生的抗体，小于 40％与13-O-脱甲基他克莫司，小于1％的环孢菌素，雷帕霉素，霉酚酸，泼尼松，氢化皮质醇和泼尼松龙。 此外，教导了使用这种抗体测量他克莫司的浓度的免疫测定。

公开(公告)号：US20110003314A1

公开(公告)日：2011-01-06

申请号：US12876837

申请日：2010-09-07

申请人： Vani Bodepudi ,  Lisa Charters ,  Rueyming Loor

发明人： Vani Bodepudi ,  Lisa Charters ,  Rueyming Loor

IPC分类号： G01N33/53 ,  C07K2/00 ,  C07K14/00 ,  C07K16/44 ,  C12N9/96 ,  C07K14/765

CPC分类号： C07K16/14 ,  G01N33/535 ,  G01N33/9446

摘要： The invention teaches derivatives of ascomycin and methods of preparing immunogens and other conjugates useful in immunoassays for quantitatively measuring concentrations of tacrolimus in patient specimens. Antibodies produced from the disclosed immunogens capable of binding to tacrolimus with cross-reactivity of no more than 5% with each of 15-O-demethyl tacrolimus, 31-O-demethyl tacrolimus, and 13,31-O-didemethyl tacrolimus, less than 40% with 13-O-demethyl tacrolimus, and less than 1% with cyclosporin, rapamycin, mycophenolic acid, prednisone, hydrocortisol, and prednisolone are described. Further, immunoassays for measuring the concentration of tacrolimus using such antibodies are taught.

摘要翻译： 本发明教导了子囊霉的衍生物以及制备用于免疫测定的免疫原和其它缀合物的方法，用于定量测量患者标本中他克莫司的浓度。 从所公开的能够与15- O-去甲基他克莫司，31-O-去甲基他克莫司和13,31-O-二甲基他克莫司结合的他克莫司的交叉反应性不超过5％的免疫原产生的抗体，小于 40％与13-O-脱甲基他克莫司，小于1％的环孢菌素，雷帕霉素，霉酚酸，泼尼松，氢化皮质醇和泼尼松龙。 此外，教导了使用这种抗体测量他克莫司的浓度的免疫测定。

公开(公告)号：US20060240496A1

公开(公告)日：2006-10-26

申请号：US11407415

申请日：2006-04-20

申请人： Lakshmi Anne ,  Vani Bodepudi

发明人： Lakshmi Anne ,  Vani Bodepudi

IPC分类号： G01N33/53 ,  C07K16/18 ,  C07K14/47

CPC分类号： G01N33/5308 ,  C07K16/44 ,  G01N2400/02

摘要： Ethyl glucuronide (“EtG”) analogs can be prepared for use as immunodiagnostic reagents and in immunodiagnostic protocols. The EtG analogs can be in the form of EtG-based immunogens, and EtG-based antigens. The EtG-based immunogens can be used for preparing anti-EtG antibodies, which can be used in immunoassays. Accordingly, improved immunoassay techniques for detecting EtG can be performed with the EtG-based antigens, and anti-EtG antibodies prepared from EtG-based immunogens.

摘要翻译： 可以制备葡萄糖醛酸葡萄糖苷（“EtG”）类似物用作免疫诊断试剂和免疫诊断方案。 EtG类似物可以是基于EtG的免疫原和EtG基抗原的形式。 基于EtG的免疫原可用于制备抗EtG抗体，可用于免疫测定。 因此，可以使用基于EtG的抗原和由EtG基免疫原制备的抗-EtG抗体来进行用于检测EtG的改进的免疫测定技术。