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    Reagents and methods for mycophenolic acid immunoassay
    1.
    发明申请
    Reagents and methods for mycophenolic acid immunoassay 有权
    霉酚酸免疫测定的试剂和方法

    公开(公告)号：US20060035297A1

    公开(公告)日：2006-02-16

    申请号：US11201992

    申请日：2005-08-11

    申请人： Vani Bodepudi ,  Amy Tsai ,  Weixing Luo ,  Rueyming Loor

    发明人： Vani Bodepudi ,  Amy Tsai ,  Weixing Luo ,  Rueyming Loor

    IPC分类号： G01N33/53 ,  C07K16/18 ,  C07K14/47

    CPC分类号： G01N33/9493 ,  C07K16/44

    摘要： The invention teaches derivatives of mycophenolic alcohol and methods of preparing immunogens and other conjugates useful in immunoassays for quantitatively measuring concentrations of mycophenolic acid (MPA) and/or active metabolites of MPA in patient specimens. Antibodies produced from the disclosed immunogens capable of binding to MPA with cross-reactivity of no more than 5% with inactive metabolites and commonly co-prescribed drugs. Further, immunoassays for measuring the concentration of MPA using such antibodies are taught.

    摘要翻译： 本发明教导霉酚醇的衍生物和制备用于免疫测定的免疫原和其它缀合物的方法，用于定量测量患者标本中的MPA和/或MPA的活性代谢物的浓度。 从所公开的能够与MPA结合的免疫原产生的抗体产生的交联反应性不超过5％，与无活性代谢物和通常共同处方的药物。 此外，教导了使用这种抗体测量MPA浓度的免疫测定。

    Hapten, immunogens and derivatives of ascomycin useful for preparation of antibodies and immunoassays
    2.
    发明申请
    Hapten, immunogens and derivatives of ascomycin useful for preparation of antibodies and immunoassays 审中-公开
    用于制备抗体和免疫测定的硫霉素，免疫原和子囊霉衍生物

    公开(公告)号：US20050176080A1

    公开(公告)日：2005-08-11

    申请号：US11049547

    申请日：2005-02-02

    申请人： Vani Bodepudi ,  Lisa Charter ,  Rueyming Loor

    发明人： Vani Bodepudi ,  Lisa Charter ,  Rueyming Loor

    IPC分类号： C07K14/47 ,  C07K16/14 ,  C07K16/18 ,  G01N33/53 ,  G01N33/535 ,  G01N33/537 ,  G01N33/543 ,  G01N33/94

    CPC分类号： C07K16/14 ,  G01N33/535 ,  G01N33/9446

    摘要： The invention teaches derivatives of ascomycin and methods of preparing immunogens and other conjugates useful in immunoassays for quantitatively measuring concentrations of tacrolimus in patient specimens. Antibodies produced from the disclosed immunogens capable of binding to tacrolimus with cross-reactivity of no more than 5% with each of 15-O-demethyl tacrolimus, 31-O-demethyl tacrolimus, and 13,31-O-didemethyl tacrolimus, less than 40% with 13-O-demethyl tacrolimus, and less than 1% with cyclosporin, rapamycin, mycophenolic acid, prednisone, hydrocortisol, and prednisolone are described. Further, immunoassays for measuring the concentration of tacrolimus using such antibodies are taught.

    摘要翻译： 本发明教导了子囊霉的衍生物以及制备用于免疫测定的免疫原和其它缀合物的方法，用于定量测量患者标本中他克莫司的浓度。 从所公开的能够与15- O-去甲基他克莫司，31-O-去甲基他克莫司和13,31-O-二甲基他克莫司结合的他克莫司的交叉反应性不超过5％的免疫原产生的抗体，小于 40％与13-O-脱甲基他克莫司，小于1％的环孢菌素，雷帕霉素，霉酚酸，泼尼松，氢化皮质醇和泼尼松龙。 此外，教导了使用这种抗体测量他克莫司的浓度的免疫测定。

    Hapten, Immunogens and Derivatives of Ascomycin Useful for Preparation of Antibodies and Immunoassays
    3.
    发明申请
    Hapten, Immunogens and Derivatives of Ascomycin Useful for Preparation of Antibodies and Immunoassays 审中-公开
    用于制备抗体和免疫测定的子囊霉素的脱蛋白，免疫原和衍生物

    公开(公告)号：US20110003314A1

    公开(公告)日：2011-01-06

    申请号：US12876837

    申请日：2010-09-07

    申请人： Vani Bodepudi ,  Lisa Charters ,  Rueyming Loor

    发明人： Vani Bodepudi ,  Lisa Charters ,  Rueyming Loor

    IPC分类号： G01N33/53 ,  C07K2/00 ,  C07K14/00 ,  C07K16/44 ,  C12N9/96 ,  C07K14/765

    CPC分类号： C07K16/14 ,  G01N33/535 ,  G01N33/9446

    摘要： The invention teaches derivatives of ascomycin and methods of preparing immunogens and other conjugates useful in immunoassays for quantitatively measuring concentrations of tacrolimus in patient specimens. Antibodies produced from the disclosed immunogens capable of binding to tacrolimus with cross-reactivity of no more than 5% with each of 15-O-demethyl tacrolimus, 31-O-demethyl tacrolimus, and 13,31-O-didemethyl tacrolimus, less than 40% with 13-O-demethyl tacrolimus, and less than 1% with cyclosporin, rapamycin, mycophenolic acid, prednisone, hydrocortisol, and prednisolone are described. Further, immunoassays for measuring the concentration of tacrolimus using such antibodies are taught.

    摘要翻译： 本发明教导了子囊霉的衍生物以及制备用于免疫测定的免疫原和其它缀合物的方法，用于定量测量患者标本中他克莫司的浓度。 从所公开的能够与15- O-去甲基他克莫司，31-O-去甲基他克莫司和13,31-O-二甲基他克莫司结合的他克莫司的交叉反应性不超过5％的免疫原产生的抗体，小于 40％与13-O-脱甲基他克莫司，小于1％的环孢菌素，雷帕霉素，霉酚酸，泼尼松，氢化皮质醇和泼尼松龙。 此外，教导了使用这种抗体测量他克莫司的浓度的免疫测定。

    Assay tray
    6.
    发明授权
    Assay tray 失效
    分析托盘

    公开(公告)号：US4681742A

    公开(公告)日：1987-07-21

    申请号：US656234

    申请日：1984-10-01

    申请人： Larry J. Johnson ,  Stephen R. Coates ,  Rueyming Loor

    发明人： Larry J. Johnson ,  Stephen R. Coates ,  Rueyming Loor

    IPC分类号： G01N33/53 ,  B01L3/00 ,  G01N35/02 ,  G01N35/10 ,  G01N33/50 ,  G01N35/00

    CPC分类号： B01L3/5085 ,  G01N35/028 ,  B01J2219/00468 ,  B01L2200/0647 ,  B01L2300/0829 ,  G01N35/1065 ,  Y10S436/809

    摘要： A machine for transferring liquids to and from the wells of assay trays in a controlled, automated manner and a solid phase assay tray for use with the machine. The machine includes a horizontally translatable table (15) that holds the tray (46), a plurality of liquid dispensing manifolds (54) for dispensing liquids into the tray wells (50) and an aspirating manifold (65) for aspirating liquid from the well. The dispensing and aspirating manifolds are mounted on a vertically translatable head (16) above the table. Each dispensing manifold is equipped with a row of dispensing tubes (56) and is connected via a pump (58) to a liquid container (62). The aspirating manifold is equipped with a row of aspirating tube (66) and is connected via a pump (69) to a waste liquid receptacle (73). A microprocessor (85) controls the movements of the table and manifolds and operates the pumps. The tray wells include means, such as sloping bottoms (74) or subwells (83), that cause the solid phase (76) to occupy a particular position in the wells and sumps (75), (84) that are positioned relative to the location of the solid phase such that they may be accessed vertically by the aspirating tubes without danger of disturbing the solid phase. The bottoms of the sumps have optically flat areas so that beams of light may be passed vertically through the liquid contents of the wells without intersecting the solid phase to make optical measurements of the liquid contents.

    摘要翻译： 用于以受控的自动化方式将液体输送到测定塔的孔中并从测定塔的孔传送液体的机器以及用于该机器的固相测定托盘。 机器包括一个保持托盘（46）的水平平移台（15），用于将液体分配到托盘孔（50）中的多个液体分配歧管（54）和用于从井中吸出液体的抽吸歧管（65） 。 分配和抽吸歧管安装在桌子上方的可垂直平移的头部（16）上。 每个分配歧管配备有一排分配管（56），并且经由泵（58）连接到液体容器（62）。 抽吸歧管配有一排抽吸管（66），并通过泵（69）连接到废液容器（73）。 微处理器（85）控制工作台和歧管的运动并操作泵。 托盘井包括使得固相（76）占据井中的特定位置的装置，例如倾斜底部（74）或底孔（83），所述井和相对于 固相的位置，使得它们可以通过抽吸管垂直地进入，而不会干扰固相的危险。 集水池的底部具有光学平坦的区域，使得光束可以垂直穿过孔的液体内容物而不与固相相交，以对液体内容物进行光学测量。

    Automated assay machine and assay tray
    7.
    发明授权
    Automated assay machine and assay tray 失效
    自动分析机和分析盘

    公开(公告)号：US4952518A

    公开(公告)日：1990-08-28

    申请号：US140888

    申请日：1987-12-28

    申请人： Larry J. Johnson ,  Stephen R. Coates ,  Rueyming Loor

    发明人： Larry J. Johnson ,  Stephen R. Coates ,  Rueyming Loor

    IPC分类号： B01L3/00 ,  G01N35/02 ,  G01N35/10

    CPC分类号： B01L3/5085 ,  G01N35/028 ,  B01J2219/00468 ,  G01N35/1065 ,  Y10T436/113332

    摘要： A machine for transferring liquids to and from the wells of assay trays in a controlled, automated manner and a solid phase assay tray for use with the machine. The machine includes a horizontally translatable table (15) that holds the tray (46), a plurality of liquid dispensing manifolds (54) for dispensing liquids into the tray wells (50) and an aspirating manifold (65) for aspirating liquid from the well. The dispensing and aspirating manifolds are mounted on a vertically translatable head (16) above the table. Each dispensing manifold is equipped with a row of dispensing tubes (56) and is connected via a pump (58) to a liquid container (62). The aspirating manifold is equipped with a row of aspirating tubes (66) and is connected via a pump (69) to a waste liquid receptacle (73). A microprocessor (85) controls the movements of the table and manifolds and operates the pumps. The tray wells include means, such as sloping bottoms (74) or subwells (83), that cause the solid phase (76) to occupy a particular position in the wells and sumps (75), (84) that are positioned relative to the location of the solid phase such that they may be accessed vertically by the aspirating tubes without danger of disturbing the solid phase. The bottoms of the sumps have optically flat areas so that beams of light may be passed vertically through the liquid contents of the wells without intersecting the solid phase to make optical measurements of the liquid contents.

    摘要翻译： 用于以受控的自动化方式将液体输送到测定塔的孔中并从测定塔的孔传送液体的机器以及用于该机器的固相测定托盘。 机器包括一个保持托盘（46）的水平平移台（15），用于将液体分配到托盘孔（50）中的多个液体分配歧管（54）和用于从井中吸出液体的抽吸歧管（65） 。 分配和抽吸歧管安装在桌子上方的可垂直平移的头部（16）上。 每个分配歧管配备有一排分配管（56），并且经由泵（58）连接到液体容器（62）。 抽吸歧管配备有一排抽吸管（66），并通过泵（69）连接到废液容器（73）。 微处理器（85）控制工作台和歧管的运动并操作泵。 托盘井包括使得固相（76）占据井中的特定位置的装置，例如倾斜底部（74）或底孔（83），所述井和相对于 固相的位置，使得它们可以通过抽吸管垂直地进入，而不会干扰固相的危险。 集水池的底部具有光学平坦的区域，使得光束可以垂直穿过孔的液体内容物而不与固相相交，以对液体内容物进行光学测量。