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1.发明申请Insect inhibitory Bacillus thuringiensis proteins, fusions, and methods of use therefor 有权昆虫抑制性苏云金芽孢杆菌蛋白，融合物及其使用方法公开(公告)号：US20070208168A1
公开(公告)日：2007-09-06
申请号：US11796742
申请日：2007-04-27
CPC分类号： A01N37/46 , A01N63/02 , C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Novel insect inhibitory proteins are disclosed comprising two different components, both of which are required for biological activity. Various methods of linking both components together, so that a single protein provides insect inhibitory activity, are disclosed. Also disclosed are novel Bacillus thuringiensis nucleic acid sequences encoding Coleopteran-inhibitory crystal proteins, designated tIC100 (29-kDa) and tlC101 (14-kDa). Also disclosed are methods of making and using nucleic acid sequences in the development of the transgenic plant cells containing the novel nucleic acid sequences disclosed herein.
摘要翻译： 公开了新的昆虫抑制蛋白，其包含两种不同的组分，这两种组分都是生物活性所必需的。 公开了将两种组分连接在一起的各种方法，使得单一蛋白提供昆虫抑制活性。 还公开了称为tIC100（29-kDa）和tlC101（14-kDa）的编码鞘翅目抑制性晶体蛋白的新型苏云金芽孢杆菌核酸序列。 还公开了在含有本文公开的新型核酸序列的转基因植物细胞的开发中制备和使用核酸序列的方法。
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公开(公告)号：US06713063B1
公开(公告)日：2004-03-30
申请号：US09636746
申请日：2000-08-11
IPC分类号： A61K3900
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的杀虫活性和更广泛的昆虫宿主范围的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白，其针对鞘翅目，双翅目和鳞翅目昆虫。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。
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公开(公告)号：US07618942B2
公开(公告)日：2009-11-17
申请号：US11998135
申请日：2007-11-28
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162 , Y10S977/902
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的杀虫活性和更广泛的昆虫宿主范围的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白，其针对鞘翅目，双翅目和鳞翅目昆虫。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。
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公开(公告)号：US20090023653A1
公开(公告)日：2009-01-22
申请号：US11998135
申请日：2007-11-28
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162 , Y10S977/902
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
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5.发明申请Plants transformed with polynucleotides encoding broad-spectrum delta-endotoxins 有权用编码广谱δ-内毒素的多核苷酸转化的植物公开(公告)号：US20060174372A1
公开(公告)日：2006-08-03
申请号：US11372065
申请日：2006-04-10
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的杀虫活性和更广泛的昆虫宿主范围的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白，其针对鞘翅目，双翅目和鳞翅目昆虫。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。
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6.发明授权Plants transformed with polynucleotides encoding broad-spectrum delta-endotoxins 有权用编码广谱δ-内毒素的多核苷酸转化的植物公开(公告)号：US07304206B2
公开(公告)日：2007-12-04
申请号：US11372065
申请日：2006-04-10
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的杀虫活性和更广泛的昆虫宿主范围的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白，其针对鞘翅目，双翅目和鳞翅目昆虫。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。
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公开(公告)号：US07070982B2
公开(公告)日：2006-07-04
申请号：US10739482
申请日：2003-12-18
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的杀虫活性和更广泛的昆虫宿主范围的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白，其针对鞘翅目，双翅目和鳞翅目昆虫。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。
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8.发明授权Insect inhibitory Bacillus thuringiensis proteins, fusions, and methods of use therefor 有权昆虫抑制性苏云金芽孢杆菌蛋白，融合物及其使用方法公开(公告)号：US07214788B2
公开(公告)日：2007-05-08
申请号：US10380077
申请日：2001-09-12
CPC分类号： A01N37/46 , A01N63/02 , C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Novel insect inhibitory proteins are disclosed comprising two different components, both of which are required for biological activity. Various methods of linking both components together, so that a single protein provides insect inhibitory activity, are disclosed. Also disclosed are novel Bacillus thuringiensis nucleic acid sequences encoding Coleopteran-inhibitory crystal proteins, designated tIC100 (29-kDa) and tIC101 (14-kDa). Also disclosed are methods of making and using nucleic acid sequences in the development of the transgenic plant cells containing the novel nucleic acid sequences disclosed herein.
摘要翻译： 公开了新的昆虫抑制蛋白，其包含两种不同的组分，这两种组分都是生物活性所必需的。 公开了将两种组分连接在一起的各种方法，使得单一蛋白提供昆虫抑制活性。 还公开了称为tIC100（29-kDa）和tIC101（14-kDa）的编码鞘翅目抑制性晶体蛋白的新型苏云金芽孢杆菌核酸序列。 还公开了在含有本文公开的新型核酸序列的转基因植物细胞的开发中制备和使用核酸序列的方法。
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9.发明授权Insect inhibitory Bacillus thuringiensis proteins, fusions, and methods of use therefor 有权昆虫抑制性苏云金芽孢杆菌蛋白，融合物及其使用方法公开(公告)号：US07655838B2
公开(公告)日：2010-02-02
申请号：US11796742
申请日：2007-04-27
CPC分类号： A01N37/46 , A01N63/02 , C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Novel insect inhibitory proteins are disclosed comprising two different components, both of which are required for biological activity. Various methods of linking both components together, so that a single protein provides insect inhibitory activity, are disclosed. Also disclosed are novel Bacillus thuringiensis nucleic acid sequences encoding Coleopteran-inhibitory crystal proteins, designated tIC100 (29-kDa) and tlC101 (14-kDa). Also disclosed are methods of making and using nucleic acid sequences in the development of the transgenic plant cells containing the novel nucleic acid sequences disclosed herein.
摘要翻译： 公开了新的昆虫抑制蛋白，其包含两种不同的组分，这两种组分都是生物活性所必需的。 公开了将两种组分连接在一起的各种方法，使得单一蛋白提供昆虫抑制活性。 还公开了称为tIC100（29-kDa）和tlC101（14-kDa）的编码鞘翅目抑制性晶体蛋白的新型苏云金芽孢杆菌核酸序列。 还公开了在含有本文公开的新型核酸序列的转基因植物细胞的开发中制备和使用核酸序列的方法。
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