公开(公告)号：US09279788B2

公开(公告)日：2016-03-08

申请号：US14672746

申请日：2015-03-30

申请人： Waters Technologies Corporation

发明人： Hongji Liu

IPC分类号： G01N30/16 ,  G01N30/34 ,  G01N30/02

CPC分类号： G01N30/16 ,  G01N30/34 ,  G01N2030/027 ,  G01N2030/347

摘要： Embodiments of the present invention feature methods and apparatus for performing chromatographic separations. The invention feature methods and control means in the form of computer programs and software that control the formation of a concentration gradient at two or more total flow rates, with an elution period occurring in a slow flow rate and at least other portions of the gradient occurring during a fast total flow rate.

摘要翻译： 本发明的实施例的特征在于进行色谱分离的方法和装置。 本发明的特征在于以计算机程序和软件的形式的方法和控制装置，其控制在两个或多个总流速下形成浓度梯度，洗脱时间以缓慢的流速发生，并且至少其它部分的梯度发生 在一个快速的总流速。

公开(公告)号：US20210123891A1

公开(公告)日：2021-04-29

申请号：US17143814

申请日：2021-01-07

申请人： Waters Technologies Corporation

发明人： Steven J. Ciavarini ,  Stanley P. Pensak, JR. ,  Jeffrey W. Finch ,  Keith Fadgen ,  Hongji Liu

IPC分类号： G01N30/34 ,  G01N30/32 ,  G05D11/13

摘要： A solvent delivery subsystem for a chromatography device performs relatively low pressure, high flow mixing of solvents to form a gradient and subsequent high pressure, low flow delivery of the gradient to the separation column. The mixing of the gradient is independent and does not interfere with the gradient delivery. To form the gradient, the outputs of an aqueous pump and an organic pump are mixed to fill a storage capillary while a downstream point from the storage capillary is vented to atmosphere. After gradient formation, the vent to atmosphere is closed, the solvent delivery system rises to high pressure, and only the aqueous pump runs for gradient delivery. To maintain integrity of the fluid stream, the solvent delivery system uses feed forward compensation and controls at least one parameter selected from the group consisting of pressure and flow in the conduit means to follow a gradual ramp.

公开(公告)号：US20150346168A1

公开(公告)日：2015-12-03

申请号：US14822527

申请日：2015-08-10

申请人： WATERS TECHNOLOGIES CORPORATION

发明人： Steven J. Ciavarini ,  Stanley P. Pensak, JR. ,  Jeffrey W. Finch ,  Keith Fadgen ,  Hongji Liu

IPC分类号： G01N30/34 ,  G01N30/32

CPC分类号： G01N30/34 ,  G01N30/32 ,  G01N2030/326 ,  G05D11/132

摘要： A solvent delivery subsystem for a chromatography device performs relatively low pressure, high flow mixing of solvents to form a gradient and subsequent high pressure, low flow delivery of the gradient to the separation column. The mixing of the gradient is independent and does not interfere with the gradient delivery. To form the gradient, the outputs of an aqueous pump and an organic pump are mixed to fill a storage capillary while a downstream point from the storage capillary is vented to atmosphere. After gradient formation, the vent to atmosphere is closed, the solvent delivery system rises to high pressure, and only the aqueous pump runs for gradient delivery. To maintain integrity of the fluid stream, the solvent delivery system uses feed forward compensation and controls at least one parameter selected from the group consisting of pressure and flow in the conduit means to follow a gradual ramp.

公开(公告)号：US20150204826A1

公开(公告)日：2015-07-23

申请号：US14672746

申请日：2015-03-30

申请人： Waters Technologies Corporation

发明人： Hongji Liu

IPC分类号： G01N30/16

CPC分类号： G01N30/16 ,  G01N30/34 ,  G01N2030/027 ,  G01N2030/347

摘要： Embodiments of the present invention feature methods and apparatus for performing chromatographic separations. The invention feature methods and control means in the form of computer programs and software that control the formation of a concentration gradient at two or more total flow rates, with an elution period occurring in a slow flow rate and at least other portions of the gradient occurring during a fast total flow rate.

摘要翻译： 本发明的实施例的特征在于进行色谱分离的方法和装置。 本发明的特征在于以计算机程序和软件的形式的方法和控制装置，其控制在两个或多个总流速下形成浓度梯度，洗脱时间以缓慢的流速发生，并且至少其它部分的梯度发生 在一个快速的总流速。