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公开(公告)号：US09206221B2
公开(公告)日：2015-12-08
申请号：US13826466
申请日：2013-03-14
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION , The Board of Trustees of the Leland Stanford Junior University
Inventor： Samuel Helmer Gellman , Pil Seok Chae , Brian Kobilka , Soren Rasmussen
CPC classification number: C07J17/005 , A61K9/1075 , C07J41/0061 , C07J71/0005
Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
Abstract translation: 将膜蛋白引入水溶液中通常需要使用洗涤剂或其他两亲剂。 本发明提供了一类新的两亲物，其中每一种都包括作为亲脂基团的多稠环体系。 这些新的两亲物相对于常规洗涤剂使溶液中的一系列膜蛋白质的稳定性提高，导致膜蛋白（包括整合的膜蛋白）的结构和功能稳定性得到改善。 因此，本发明提供用于生物化学操作和膜蛋白表征的新型两亲物。 这些两亲物显示出有利于膜蛋白的行为，并可用于帮助膜蛋白的溶解，分离，纯化，稳定，结晶和/或结构测定。
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公开(公告)号：US10316057B2
公开(公告)日：2019-06-11
申请号：US14337496
申请日：2014-07-22
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： Pil Seok Chae , Samuel Helmer Gellman
IPC: A61K9/00 , C07J43/00 , C07J41/00 , A61K47/28 , C07K1/14 , A61K47/26 , C07J9/00 , C07J31/00 , C07J51/00 , A61B90/00 , A61B34/30 , A61K9/107
Abstract: The invention provides tandem facial amphiphiles for biochemical manipulations and characterization of membrane proteins, such as intrinsic membrane proteins. Members of this new family display favorable behavior with several membrane proteins. These amphiphiles can form relatively small micelles, and small changes in amphiphile chemical structures can result in large changes in their physical properties. The tandem facial amphiphiles can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
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公开(公告)号：US09556217B2
公开(公告)日：2017-01-31
申请号：US14931225
申请日：2015-11-03
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION , The Board of Trustees of the Leland Stanford Junior University
Inventor： Samuel Helmer Gellman , Pil Seok Chae , Brian Kobilka , Soren Rasmussen
CPC classification number: C07J17/005 , A61K9/1075 , C07J41/0061 , C07J71/0005
Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
Abstract translation: 将膜蛋白引入水溶液中通常需要使用洗涤剂或其他两亲剂。 本发明提供了一类新的两亲物，其中每一种都包括作为亲脂基团的多稠环体系。 这些新的两亲物相对于常规洗涤剂使溶液中的一系列膜蛋白质的稳定性提高，导致膜蛋白（包括整合的膜蛋白）的结构和功能稳定性得到改善。 因此，本发明提供用于生物化学操作和膜蛋白表征的新型两亲物。 这些两亲物显示出有利于膜蛋白的行为，并可用于帮助膜蛋白的溶解，分离，纯化，稳定，结晶和/或结构测定。
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4.发明授权Cholate and deoxycholate-based amphiphiles for membrane protein manipulation 有权用于膜蛋白操作的胆酸和脱氧胆酸盐基两亲物公开(公告)号：US09255122B2
公开(公告)日：2016-02-09
申请号：US14556544
申请日：2014-12-01
Applicant: Wisconsin Alumni Research Foundation
Inventor： Samuel Helmer Gellman , Pil Seok Chae
IPC: C07C257/00 , C07C263/00 , C07K1/14 , C07J41/00
CPC classification number: C07K1/14 , C07J41/00 , C07J41/0061 , C07K1/145
Abstract: Disclosed are compounds and methods for manipulating proteins in general and membrane proteins in particular. The compounds can be prepared from cholic acid, deoxycholic acid, lithocholic acid, or derivatives thereof. The compounds typically possess critical micelle concentrations lower than those of known detergents such as CHAPS and CHAPSO. Accordingly, lower amounts of the compounds are required for effective solubilization of membrane proteins. The compounds can be used aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
Abstract translation: 公开的是一般操作蛋白质和特别是膜蛋白质的化合物和方法。 该化合物可以由胆酸，脱氧胆酸，石胆酸或其衍生物制备。 这些化合物通常具有低于已知洗涤剂如CHAPS和CHAPSO的临界胶束浓度。 因此，为了有效溶解膜蛋白质，需要较低量的化合物。 这些化合物可用于帮助膜蛋白的溶解，分离，纯化，稳定化，结晶和/或结构测定。
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Patent Agency Ranking
公开(公告)号：US20150011739A1
公开(公告)日：2015-01-08
申请号：US14337496
申请日：2014-07-22
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： Pil Seok Chae , Samuel Helmer GELLMAN
CPC classification number: C07J43/003 , A61B34/30 , A61B90/361 , A61B2034/301 , A61B2090/3782 , A61K9/107 , A61K47/26 , A61K47/28 , C07J9/00 , C07J31/006 , C07J41/0061 , C07J51/00 , C07K1/145
Abstract: The invention provides tandem facial amphiphiles for biochemical manipulations and characterization of membrane proteins, such as intrinsic membrane proteins. Members of this new family display favorable behavior with several membrane proteins. These amphiphiles can form relatively small micelles, and small changes in amphiphile chemical structures can result in large changes in their physical properties. The tandem facial amphiphiles can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
Abstract translation: 本发明提供用于生物化学操作和膜蛋白（例如内在膜蛋白）表征的串联面部两亲物。 这个新家庭的成员显示有几种膜蛋白的有利行为。 这些两亲物可以形成相对较小的胶束，两亲化学结构的小变化可导致其物理性质的大的变化。 串联面部两亲物可用于帮助膜蛋白的溶解，分离，纯化，稳定化，结晶和/或结构测定。
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公开(公告)号：US08815263B2
公开(公告)日：2014-08-26
申请号：US13669198
申请日：2012-11-05
Applicant: Wisconsin Alumni Research Foundation
Inventor： Pil Seok Chae , Samuel Helmer Gellman
CPC classification number: C07J43/003 , A61B34/30 , A61B90/361 , A61B2034/301 , A61B2090/3782 , A61K9/107 , A61K47/26 , A61K47/28 , C07J9/00 , C07J31/006 , C07J41/0061 , C07J51/00 , C07K1/145
Abstract: The invention provides tandem facial amphiphiles for biochemical manipulations and characterization of membrane proteins, such as intrinsic membrane proteins. Members of this new family display favorable behavior with several membrane proteins. These amphiphiles can form relatively small micelles, and small changes in amphiphile chemical structures can result in large changes in their physical properties. The tandem facial amphiphiles can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
Abstract translation: 本发明提供用于生物化学操作和膜蛋白（例如内在膜蛋白）表征的串联面部两亲物。 这个新家庭的成员显示有几种膜蛋白的有利行为。 这些两亲物可以形成相对较小的胶束，并且两亲化学结构的小变化可导致其物理性质的大的变化。 串联面部两亲物可用于帮助膜蛋白的溶解，分离，纯化，稳定化，结晶和/或结构测定。
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公开(公告)号：US09540409B2
公开(公告)日：2017-01-10
申请号：US13961757
申请日：2013-08-07
Applicant: Wisconsin Alumni Research Foundation , The Board of Trustees of the Leland Stanford Junior University
Inventor： Samuel Helmer Gellman , Pil Seok Chae , Brian Kobilka , Soren Rasumssen
Abstract: The invention provides amphiphilic compounds and methods for manipulating membrane proteins. Compounds of the invention, for example, the compounds of Formulas I-XIX, can be prepared from readily available starting materials. The amphiphilic compounds can manipulate membrane protein at relatively low concentrations compared to many known detergents. The compounds can be used to aid the isolation of membrane proteins, for example, to aid their solubilization and/or purification. The compounds can also be used to aid the functional and structural determination of membrane proteins, including their stabilization and crystallization.
Abstract translation: 本发明提供两亲化合物和操作膜蛋白的方法。 本发明的化合物，例如式I-XIX的化合物可以由容易获得的原料制备。 与许多已知的洗涤剂相比，两亲化合物可以以相对低的浓度操纵膜蛋白。 该化合物可用于帮助分离膜蛋白，例如帮助其溶解和/或纯化。 该化合物也可用于帮助膜蛋白的功能和结构测定，包括其稳定和结晶。
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8.发明申请CHOLATE AND DEOXYCHOLATE-BASED AMPHIPHILES FOR MEMBRANE PROTEIN MANIPULATION 有权用于膜蛋白操作的基于脱乙酰壳多糖和去氧基化物的培养基公开(公告)号：US20150203441A1
公开(公告)日：2015-07-23
申请号：US14556544
申请日：2014-12-01
Applicant: Wisconsin Alumni Research Foundation
Inventor： Samuel Helmer Gellman , Pil Seok Chae
IPC: C07C233/16 , C07K1/14
CPC classification number: C07K1/14 , C07J41/00 , C07J41/0061 , C07K1/145
Abstract: Disclosed are compounds and methods for manipulating proteins in general and membrane proteins in particular. The compounds can be prepared from cholic acid, deoxycholic acid, lithocholic acid, or derivatives thereof. The compounds typically possess critical micelle concentrations lower than those of known detergents such as CHAPS and CHAPSO. Accordingly, lower amounts of the compounds are required for effective solubilization of membrane proteins. The compounds can be used aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
Abstract translation: 公开的是一般操作蛋白质和特别是膜蛋白质的化合物和方法。 该化合物可以由胆酸，脱氧胆酸，石胆酸或其衍生物制备。 这些化合物通常具有低于已知洗涤剂如CHAPS和CHAPSO的临界胶束浓度。 因此，为了有效溶解膜蛋白质，需要较低量的化合物。 这些化合物可用于帮助膜蛋白的溶解，分离，纯化，稳定化，结晶和/或结构测定。
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公开(公告)号：US20130266656A1
公开(公告)日：2013-10-10
申请号：US13826466
申请日：2013-03-14
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： Samuel Helmer Gellman , Pil Seok Chae , Brian Kobilka , Soren Rasmussen
CPC classification number: C07J17/005 , A61K9/1075 , C07J41/0061 , C07J71/0005
Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
Abstract translation: 将膜蛋白引入水溶液中通常需要使用洗涤剂或其他两亲剂。 本发明提供了一类新的两亲物，其中每一种都包括作为亲脂基团的多稠环体系。 这些新的两亲物相对于常规洗涤剂使溶液中的一系列膜蛋白质的稳定性提高，导致膜蛋白（包括整合的膜蛋白）的结构和功能稳定性得到改善。 因此，本发明提供用于生物化学操作和膜蛋白表征的新型两亲物。 这些两亲物显示出有利于膜蛋白的行为，并可用于帮助膜蛋白的溶解，分离，纯化，稳定，结晶和/或结构测定。
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