公开(公告)号：US07993919B2

公开(公告)日：2011-08-09

申请号：US10579988

申请日：2004-11-18

申请人： Warren J. Leonard ,  Peter Lipsky ,  Herbert C. Morse, III ,  Catherine Rachel Ettinger ,  Rosanne Spolski

发明人： Warren J. Leonard ,  Peter Lipsky ,  Herbert C. Morse, III ,  Catherine Rachel Ettinger ,  Rosanne Spolski

IPC分类号： C12N5/00 ,  C12N5/071

CPC分类号： G01N33/5052 ,  A61K35/17 ,  A61K38/20 ,  A61K2035/124 ,  A61K2039/5158 ,  C12N5/0635 ,  C12N2501/052 ,  C12N2501/23 ,  C12N2501/50 ,  C12N2501/52 ,  G01N2333/54

摘要： A method is disclosed herein for inducing differentiation of a B cell progenitor into a memory B cells and/or a plasma cell. The method includes contacting a population of cells including a mature B cell or a B cell progenitor with an effective amount of IL-21, and isolating memory B cells or plasma cells. In one embodiment, the B cell progenitor is an immature B cell. A method is also disclosed for enhancing an immune response. The method includes contacting a population of cells including a B cell progenitor with an effective amount of IL-21, and isolating memory B cells or plasma cells. The memory B cells arid/or the plasma cell are then introduced into the subject to enhance the immune response. A method is also disclosed for treating a subject with a condition comprising a specific deficiency of at least one of memory B cells and plasma cells. A method is disclosed for identifying an agent with a physiological effect on one or more of a memory B cell and a plasma cell differentiation. A method is also disclosed for identifying agents that inhibit an activity of IL-21.

摘要翻译： 本文公开了一种用于诱导B细胞祖细胞分化为记忆B细胞和/或浆细胞的方法。 该方法包括使包含成熟B细胞或B细胞祖细胞的细胞群与有效量的IL-21接触，并分离记忆B细胞或浆细胞。 在一个实施方案中，B细胞祖细胞是未成熟B细胞。 还公开了一种用于增强免疫应答的方法。 该方法包括使包含B细胞祖细胞的细胞群与有效量的IL-21接触，并分离记忆B细胞或浆细胞。 然后将记忆B细胞和/或浆细胞引入受试者以增强免疫应答。 还公开了一种用于治疗患有包含存储B细胞和浆细胞中的至少一种的特定缺陷的病症的受试者的方法。 公开了用于鉴定对存储B细胞和浆细胞分化中的一种或多种具有生理作用的药剂的方法。 还公开了用于鉴定抑制IL-21活性的试剂的方法。

公开(公告)号：US20060057123A1

公开(公告)日：2006-03-16

申请号：US11197221

申请日：2005-08-03

申请人： Catherine Ettinger ,  Peter Lipsky ,  Warren Leonard ,  Rosanne Spolski ,  Herbert Morse

发明人： Catherine Ettinger ,  Peter Lipsky ,  Warren Leonard ,  Rosanne Spolski ,  Herbert Morse

IPC分类号： A61K35/14 ,  C12N5/08

CPC分类号： G01N33/5052 ,  A61K35/17 ,  A61K38/20 ,  A61K2035/124 ,  A61K2039/5158 ,  C12N5/0635 ,  C12N2501/052 ,  C12N2501/23 ,  C12N2501/50 ,  C12N2501/52 ,  G01N2333/54

摘要： A method is disclosed herein for inducing differentiation of a B cell progenitor into a memory B cells and/or a plasma cell. The method includes contacting a population of cells including a mature B cell or a B cell progenitor with an effective amount of IL-21, and isolating memory B cells or plasma cells. In one embodiment, the B cell progenitor is an immature B cell. A method is also disclosed for enhancing an immune response. The method includes contacting a population of cells including a B cell progenitor with an effective amount of IL-21, and isolating memory B cells or plasma cells. The memory B cells and/or the plasma cell are then introduced into the subject to enhance the immune response. A method is also disclosed for treating a subject with a condition comprising a specific deficiency of at least one of memory B cells and plasma cells. A method is disclosed for identifying an agent with a physiological effect on one or more of a memory B cell and a plasma cell differentiation. A method is also disclosed for identifying agents that inhibit an activity of IL-21. Methods are also disclosed for inducing apoptosis of a B cell and for decreasing the number of B cells. A method is also described for producing a B cell hybridoma.

摘要翻译： 本文公开了一种用于诱导B细胞祖细胞分化为记忆B细胞和/或浆细胞的方法。 该方法包括使包含成熟B细胞或B细胞祖细胞的细胞群与有效量的IL-21接触，并分离记忆B细胞或浆细胞。 在一个实施方案中，B细胞祖细胞是未成熟B细胞。 还公开了一种用于增强免疫应答的方法。 该方法包括使包含B细胞祖细胞的细胞群与有效量的IL-21接触，并分离记忆B细胞或浆细胞。 然后将记忆B细胞和/或浆细胞引入受试者以增强免疫应答。 还公开了一种用于治疗患有包含存储B细胞和浆细胞中的至少一种的特定缺陷的病症的受试者的方法。 公开了用于鉴定对存储B细胞和浆细胞分化中的一种或多种具有生理作用的药剂的方法。 还公开了用于鉴定抑制IL-21活性的试剂的方法。 还公开了诱导B细胞凋亡和减少B细胞数量的方法。 还描述了用于产生B细胞杂交瘤的方法。

公开(公告)号：US20070003515A1

公开(公告)日：2007-01-04

申请号：US10579988

申请日：2004-11-18

申请人： Warren Leonard ,  Peter Lipsky ,  Herbert Morse ,  Catherine Ettinger ,  Rosanne Spolski

发明人： Warren Leonard ,  Peter Lipsky ,  Herbert Morse ,  Catherine Ettinger ,  Rosanne Spolski

IPC分类号： A61K38/20 ,  A61K35/14 ,  G01N33/567

CPC分类号： G01N33/5052 ,  A61K35/17 ,  A61K38/20 ,  A61K2035/124 ,  A61K2039/5158 ,  C12N5/0635 ,  C12N2501/052 ,  C12N2501/23 ,  C12N2501/50 ,  C12N2501/52 ,  G01N2333/54

摘要： A method is disclosed herein for inducing differentiation of a B cell progenitor into a memory B cells and/or a plasma cell. The method includes contacting a population of cells including a mature B cell or a B cell progenitor with an effective amount of IL-21, and isolating memory B cells or plasma cells. In one embodiment, the B cell progenitor is an immature B cell. A method is also disclosed for enhancing an immune response. The method includes contacting a population of cells including a B cell progenitor with an effective amount of IL-21, and isolating memory B cells or plasma cells. The memory B cells arid/or the plasma cell are then introduced into the subject to enhance the immune response. A method is also disclosed for treating a subject with a condition comprising a specific deficiency of at least one of memory B cells and plasma cells. A method is disclosed for identifying an agent with a physiological effect on one or more of a memory B cell and a plasma cell differentiation. A method is also disclosed for identifying agents that inhibit an activity of IL-21.

摘要翻译： 本文公开了一种用于诱导B细胞祖细胞分化为记忆B细胞和/或浆细胞的方法。 该方法包括使包含成熟B细胞或B细胞祖细胞的细胞群与有效量的IL-21接触，并分离记忆B细胞或浆细胞。 在一个实施方案中，B细胞祖细胞是未成熟B细胞。 还公开了一种用于增强免疫应答的方法。 该方法包括使包含B细胞祖细胞的细胞群与有效量的IL-21接触，并分离记忆B细胞或浆细胞。 然后将记忆B细胞和/或浆细胞引入受试者以增强免疫应答。 还公开了一种用于治疗患有包含存储B细胞和浆细胞中的至少一种的特定缺陷的病症的受试者的方法。 公开了用于鉴定对存储B细胞和浆细胞分化中的一种或多种具有生理作用的药剂的方法。 还公开了用于鉴定抑制IL-21活性的试剂的方法。

公开(公告)号：US20050193434A1

公开(公告)日：2005-09-01

申请号：US11027868

申请日：2004-12-30

申请人： Warren Leonard ,  Katsutoshi Ozaki ,  Rosanne Spolski ,  Herbert Morse ,  Peter Lipsky ,  Derry Roopenian

发明人： Warren Leonard ,  Katsutoshi Ozaki ,  Rosanne Spolski ,  Herbert Morse ,  Peter Lipsky ,  Derry Roopenian

IPC分类号： A01K67/027 ,  C07K14/54 ,  C07K14/715 ,  C12N20060101 ,  C12N15/85 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C07K14/7155 ,  A01K67/0276 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0381 ,  C07K14/5406 ,  C12N15/8509

摘要： A transgenic mouse is disclosed herein whose somatic and germ cells comprise a disrupted IL-21 receptor gene, the disruption being sufficient to inhibit the binding of IL-21 to an IL-21 receptor, and a disrupted IL-4 gene, the disruption being sufficient to inhibit the production of IL-4 or the binding of IL-4 to the IL-4 receptor. A mouse homozygous for the disrupted IL-21 receptor gene and homozygous for the disrupted IL-4 gene has diminished B cell function. A method is disclosed for altering a B cell activity. The method includes administering a therapeutically effective amount of an agent that interferes with the interaction of IL-21 with an IL-21 receptor, thereby altering the B cell activity. A method is also disclosed for of treating a subject with Job's disorder or atopic disease. A method is also disclosed for treating or preventing an allergic reaction in a subject. A method is also disclosed for treating a subject with an autoimmune or antibody mediated disorder.

摘要翻译： 本文公开了一种转基因小鼠，其体细胞和生殖细胞包含破坏的IL-21受体基因，所述破坏足以抑制IL-21与IL-21受体的结合以及破坏的IL-4基因，所述破坏是 足以抑制IL-4的产生或IL-4与IL-4受体的结合。 对于破坏的IL-21受体基因纯合的小鼠和破坏的IL-4基因是纯合的，具有减少的B细胞功能。 公开了用于改变B细胞活性的方法。 该方法包括施用治疗有效量的干扰IL-21与IL-21受体相互作用的试剂，由此改变B细胞活性。 还公开了用于治疗患有约瑟病或特应性疾病的受试者的方法。 还公开了用于治疗或预防受试者的过敏反应的方法。 还公开了用自身免疫或抗体介导的病症治疗受试者的方法。

公开(公告)号：US06203793B1

公开(公告)日：2001-03-20

申请号：US08832464

申请日：1997-04-02

申请人： Peter Lipsky ,  William Mileski

发明人： Peter Lipsky ,  William Mileski

IPC分类号： A61K39345

CPC分类号： C07K16/2845 ,  A61K38/00 ,  C07K16/2821 ,  C07K16/2854 ,  C07K16/2884

摘要： Disclosed is a method for treating tissue necrosis (loss) in an animal. Tissue necrosis is treated by providing the subject with an anti-adhesion cell agent capable of binding an ICAM-1 antigen, a CD18 antigen, an L-selectin agent, a CD44 antigen, a P-selectin antigen, a VCAM-1 antigen, an ICAM-2 antigen, or a fragment thereof. Also provided is a method for preventing thermal-related tissue loss or necrosis, particularly the thermal injury and progressive tissue necrosis which results from a burn injury. Thermal-related tissue injury surrounding a thermal injury site is inhibited or prevented by providing an animal with an anti-adhesion agent, such as an anti-CD18 antibody, an anti-ICAM-1 antibody, or a mixture or fragment thereof. A method for inhibiting/preventing scarring attendant healing of a thermal injury is also disclosed.

摘要翻译： 公开了一种治疗动物组织坏死（损失）的方法。 通过向受试者提供能够结合ICAM-1抗原，CD18抗原，L-选择素试剂，CD44抗原，P-选择蛋白抗原，VCAM-1抗原的抗粘附细胞试剂来治疗组织坏死， ICAM-2抗原或其片段。 还提供了防止热相关组织损失或坏死的方法，特别是由烧伤引起的热损伤和进行性组织坏死。 通过向动物提供抗粘连剂，例如抗CD18抗体，抗ICAM-1抗体或其混合物或片段，可以抑制或预防围绕热损伤部位的热相关组织损伤。 还公开了用于抑制/预防热损伤的瘢痕形成伴随愈合的方法。