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公开(公告)号：US10513563B2
公开(公告)日：2019-12-24
申请号：US13476812
申请日：2012-05-21
申请人： Wei-Chiang Shen , Yan Wang , Jennica Krankel
发明人： Wei-Chiang Shen , Yan Wang , Jennica Krankel
摘要： The present invention provides compositions useful as prodrugs and methods for making the same. The compositions include a fusion protein having a first delivery domain and a second protein precursor domain linked together via a linker sequence. The delivery domain is a protein capable of facilitating entry to a target cells via the endocytotic pathway, such as transferrin. The protein precursor is a prohormone or a profactor, such as proinsulin. Methods of this invention include the steps of selecting a protein suitable as the delivery domain, constructing a vector to encode the fusion protein, and expressing the fusion protein in a suitable expression host. Also disclosed is a method for targeted-delivery of prodrugs to livers and a method of reducing hepatic glucose production.
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公开(公告)号：US11952603B2
公开(公告)日：2024-04-09
申请号：US16108930
申请日：2018-08-22
申请人： Kenneth S. Manning , Yan Wang , Dustin Nielsen , Ryan Ruf , Colin Crowley , Jon Restivo , Dana Spangenberg , Karla Anhalt , Mark Romich , Anisha Akula , Carmen Fritz
发明人： Kenneth S. Manning , Yan Wang , Dustin Nielsen , Ryan Ruf , Colin Crowley , Jon Restivo , Dana Spangenberg , Karla Anhalt , Mark Romich , Anisha Akula , Carmen Fritz
CPC分类号： C12N9/94 , A61K9/00 , A61K31/327 , A61K35/39 , A61K38/46 , C12N7/00 , C12N2750/10063 , C12N2750/14363 , C12N2770/32263 , A61K35/39 , A61K2300/00 , A61K38/46 , A61K2300/00 , A61K31/327 , A61K2300/00
摘要： A pancreatin preparation having reduced viral infectivity includes one or more pancreatin enzymes and peracetic acid (PAA). At least one pancreatin enzyme may be derived from an animal source such as a porcine pancreas gland. In a particular embodiment, the one or more pancreatin enzymes are selected from the group consisting of lipases, proteases, and amylases.
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公开(公告)号：US11579086B2
公开(公告)日：2023-02-14
申请号：US17496262
申请日：2021-10-07
申请人： Yan Wang , Cory McCluskey
发明人： Yan Wang , Cory McCluskey
摘要： Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.
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公开(公告)号：US20220026363A1
公开(公告)日：2022-01-27
申请号：US17496285
申请日：2021-10-07
申请人： Yan Wang , Cory McCluskey
发明人： Yan Wang , Cory McCluskey
摘要： Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.
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公开(公告)号：US20220026362A1
公开(公告)日：2022-01-27
申请号：US17496262
申请日：2021-10-07
申请人： Yan Wang , Cory McCluskey
发明人： Yan Wang , Cory McCluskey
摘要： Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.
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公开(公告)号：US10993305B2
公开(公告)日：2021-04-27
申请号：US16722070
申请日：2019-12-20
申请人： Yan Wang
发明人： Yan Wang
摘要： A wireless receiving circuit and a wireless control device. The wireless receiving circuit includes a signal receiving module, a Bluetooth signal processing module, a voltage stabilization module, and an output control module. An output end of the signal receiving module is connected with an input end of the Bluetooth signal processing module, and a voltage end of the Bluetooth signal processing module is connected with an output end of the voltage stabilization module. An output end of the Bluetooth signal processing module is connected with an input end of the output control module. The signal receiving module is to receive an external Bluetooth signal and is to transmit the signal to the Bluetooth signal processing module. The Bluetooth signal processing module is to process the external Bluetooth signal and is to transmit a control signal to the output control module.
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公开(公告)号：US20200277654A1
公开(公告)日：2020-09-03
申请号：US16875381
申请日：2020-05-15
申请人： Yan Wang
发明人： Yan Wang
IPC分类号： C12Q1/686 , C12Q1/6827 , C12Q1/6876 , C12Q1/6848
摘要： Disclosed are methods for detecting DNA mutations of target genes in a DNA sample by combining single-molecule clonal amplification and mutant primer specific extension detection. In the method, thousands and millions of DNA molecules are locally amplified to form immobilized DNA clusters of identical sequences. Mutation specific primers are used to anneal to the mutant sequences in the DNA clusters and are extended by DNA polymerase to make labeled DNA strands. The labeled DNA clusters are detected to identify the DNA clusters of mutant sequences. This method enables detection of single mutation molecule and direct enumeration of mutation molecules in the sample. Once generated from a DNA sample, the immobilized DNA clusters can be reused many times for detection of different mutations or sequences of interest. Methods for determining differential gene expression and chromosome copy number variation are also disclosed.
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