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公开(公告)号：US5701699A
公开(公告)日：1997-12-30
申请号：US485986
申请日：1995-06-07
摘要： The present invention provides manufactured seeds and related compositions and methods. The manufactured seeds comprise a unit of a totipotent plant tissue, preferably in contact with a hydrated gel. Preferably, the shoot of the germinating embryo is enclosed in a shoot restraint that is resistant to penetration by the growing shoot. The manufactured seed may be at least partially surrounded by a protective manufactured seed coat. The gel or the seed coat can include various additives, such as nutrients, antibiotics, or plant growth regulators.
摘要翻译： 本发明提供制造的种子及相关的组合物和方法。 制造的种子包括全能植物组织的单位，优选与水合凝胶接触。 优选地，发芽胚芽的芽被封闭在能够通过生长的枝条穿透的枝条约束条中。 制造的种子可以至少部分地被保护性制成的种皮包围。 凝胶或种皮可以包括各种添加剂，例如营养物，抗生素或植物生长调节剂。
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公开(公告)号：US07207139B2
公开(公告)日：2007-04-24
申请号：US10981438
申请日：2004-11-03
IPC分类号： A01C1/06
CPC分类号： B65G47/91 , A01C11/00 , B65G2201/02 , B65G2201/0202 , B65G2201/0229
摘要： A vacuum pick-up device 20 includes a device body 22 having a pick-up tip opening 78 to which vacuum pressure is supplied. The vacuum pick-up device 20 further includes a mechanical release assisting device 36 that assists in the release of a grasped object contemporaneously with or slightly subsequent to the cessation of vacuum pressure. A manifold 38 may be included to efficiently and effectively supply vacuum pressure to the device body 22 from a vacuum pressure source.
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公开(公告)号：US5236469A
公开(公告)日：1993-08-17
申请号：US604656
申请日：1990-10-26
摘要： An analog of botanic seed is disclosed which comprises a plant embryo preferably encapsulated, or at least in contact with, a hydrated oxygenated gel. The gel can be oxygenated by passing oxygen gas through a gel solution before curing the gel or by exposing the gel to oxygen gas after curing. The gel is preferably oxygenated by adding to an uncured gel solution a suitably stabilized emulsion of a perfluorocarbon compound or a silicon oil, which compounds are capable of absorbing large amounts of oxygen, and are non-toxic and inert. An analog of botanic see can further comprise an outer shell at least partially surrounding the gel and embryo, thereby forming a capsule. The outer shell preferably is shaped to aid the radicle of a germinating embryo in protrusively rupturing the capsule, thereby facilitating successful germination and minimizing incidence of seedling malformation. Other shell materials are selected to provide requisite rigidity to the capsule while imparting minimal restriction to successful germination.
摘要翻译： 公开了植物种子的类似物，其包含优选包封或至少与水合氧化凝胶接触的植物胚。 在固化凝胶之前，通过使氧气通过凝胶溶液，或者在固化后将凝胶暴露于氧气，可以使凝胶氧化。 优选通过向未固化的凝胶溶液加入适当稳定的全氟化碳化合物或硅油的乳液，该化合物能够吸收大量的氧，并且是无毒的和惰性的，该凝胶是优选的。 植物学类似物可以进一步包含至少部分地围绕凝胶和胚胎的外壳，从而形成胶囊。 外壳优选地被成形为辅助发芽胚胎的胚根突出地破裂胶囊，从而有助于成功发芽并使幼苗畸形的发生率最小化。 选择其它壳材料以为胶囊提供必要的刚性，同时最小限度地限制成功发芽。
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4.发明授权Methods of preparing nutritive media for growth and/or germination of plant embryos 有权制备用于植物胚胎生长和/或萌发的营养培养基的方法公开(公告)号：US08871514B2
公开(公告)日：2014-10-28
申请号：US12874089
申请日：2010-09-01
CPC分类号： C12N5/04
摘要： In one aspect, a method is provided for producing an improved nutritive medium comprising an adsorbent material for culturing plant embryos.
摘要翻译： 一方面，提供了一种用于生产包含用于培养植物胚胎的吸附剂材料的改进的营养培养基的方法。
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公开(公告)号：US20130000189A1
公开(公告)日：2013-01-03
申请号：US13533260
申请日：2012-06-26
IPC分类号： A01C1/06
CPC分类号： A01H4/006
摘要： The present disclosure includes a shoot restraint for use with manufactured seeds comprising. In some embodiments, the shoot restraint includes a substantially porous material having a top surface and a bottom surface. The substantially porous material is formed to define a substantially cylindrical upper portion having an upper diameter; and a substantially cylindrical lower portion having a lower diameter. The upper diameter is larger than the lower diameter. A parabolic cavity extends from the top surface to the bottom surface through the upper portion and at least partially into the lower portion.
摘要翻译： 本公开包括与制造的种子一起使用的拍摄约束，包括。 在一些实施例中，拍摄约束包括具有顶表面和底表面的基本上多孔的材料。 形成基本上多孔的材料以限定具有上部直径的基本上圆柱形的上部; 以及具有较小直径的基本上圆柱形的下部。 上直径大于下直径。 抛物面空腔从上表面延伸到底表面，并且至少部分地进入下部。
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公开(公告)号：US07131234B2
公开(公告)日：2006-11-07
申请号：US10982952
申请日：2004-11-04
申请人： William C. Carlson , Jeffrey E. Hartle , Michael K. McKinnis , Curtis A. Bryan , Patrick Gaillard , Edwin Hirahara , Paul G. Gaddis , Katherine M. Salatas
发明人： William C. Carlson , Jeffrey E. Hartle , Michael K. McKinnis , Curtis A. Bryan , Patrick Gaillard , Edwin Hirahara , Paul G. Gaddis , Katherine M. Salatas
摘要： A combination end seal and cotyledon restraint (20) for an artificial seed (22) is provided. The combination end seal and cotyledon restraint includes a cap portion (30) sized to substantially seal one end of an artificial seed and a cotyledon restraint (32) unitarily formed with the cap portion.
摘要翻译： 提供了一种用于人造种子（22）的组合端封和子叶约束（20）。 组合端部密封和子叶约束包括尺寸适于基本上密封人造种子的一端的盖部分（30）和与盖部分整体形成的子叶约束（32）。
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公开(公告)号：US06119395A
公开(公告)日：2000-09-19
申请号：US16827
申请日：1998-01-30
CPC分类号： A01H4/006
摘要： The present invention provides manufactured seeds and related compositions and methods. The manufactured seeds comprise a unit of a totipotent plant tissue enclosed in a protective manufactured seed coat that includes an orifice sealed by a lid. The lid has a burst strength and architecture (e.g., a dome or nipple) similar to those of analogous structures in a natural botanic seed.
摘要翻译： 本发明提供制造的种子及相关的组合物和方法。 所制造的种子包含一个全部植物组织的单位，封闭在保护性制造的种皮中，其包括通过盖子密封的孔口。 盖子具有类似于天然植物种子类似结构的爆裂强度和结构（例如，圆顶或乳头）。
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公开(公告)号：US5486218A
公开(公告)日：1996-01-23
申请号：US69560
申请日：1993-06-01
摘要： An analog of botanic seed is disclosed which comprises a plant embryo preferably encapsulated, or at least in contact with, a hydrated oxygenated gel. The gel can be oxygenated by passing oxygen gas through a gel solution before curing the gel or by exposing the gel to oxygen gas after curing. The gel is preferably oxygenated by adding to an uncured gel solution a suitably stabilized emulsion of a perfluorocarbon compound or a silicone oil, which compounds are capable of absorbing large amounts of oxygen, and are non-toxic and inert. An analog of botanic seed can further comprise an outer shell at least partially surrounding the gel and embryo, thereby forming a capsule. The outer shell preferably is shaped to aid the radicle of a germinating embryo in protrusively rupturing the capsule, thereby facilitating successful germination and minimizing incidence of seedling malformation. Other shell materials are selected to provide requisite rigidity to the capsule while imparting minimal restriction to successful germination.
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公开(公告)号：US08789311B2
公开(公告)日：2014-07-29
申请号：US12243089
申请日：2008-10-01
CPC分类号： A01H4/006
摘要： A manufactured seed is provided. The manufactured seed includes a seed shell housing a nutritive media and a restraint disposed within the seed shell. The restraint includes a cavity. The manufactured seed also includes an embryo disposed within the cavity and a fill material disposed within the cavity. The manufactured further includes a seal disposed on the seed shell to seal the embryo within the seed shell.
摘要翻译： 提供制造的种子。 制造的种子包括容纳营养介质和设置在种子壳内的约束的种子壳。 约束包括一个空腔。 制造的种子还包括设置在空腔内的胚胎和设置在空腔内的填充材料。 制造的还包括设置在种子壳上以密封种子壳内的胚胎的密封件。
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公开(公告)号：US08466086B2
公开(公告)日：2013-06-18
申请号：US10371612
申请日：2003-02-20
申请人： William C. Carlson , Jeffrey E. Hartle , James A. Grob , Katherine M. Salatas , Mollie K. Heilesen
发明人： William C. Carlson , Jeffrey E. Hartle , James A. Grob , Katherine M. Salatas , Mollie K. Heilesen
CPC分类号： A01H4/006
摘要： Manufactured seeds are disclosed that comprise a unit of totipotent plant tissue and a nutritive medium. The nutritive medium can contain a number of different components selected from the following: a gel solute, charcoal, a carbon source, urea, KNO3, NH4NO3, CuCl2, CuSO4, KI, KH2PO4, CaCl2, MgSO4, Na2EDTA, FeSO4, ferric citrate, MnSO4, MnCl2, H3BO3, ZnSO4, CoCl2, Na2MoO4, (NH4)2MoO4, thiamine, riboflavin, pyridoxine, HCl, Ca-pantothenate, nicotinic acid, biotin, folic acid, and myo-inositol. The nutritive medium can also include any of various protein amino acids, any of various polyamines, any of various oxygen-absorbing compounds, any of various non-protein amino acids, and/or a smoke suspension.
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