公开(公告)号：US20050155113A1

公开(公告)日：2005-07-14

申请号：US10895791

申请日：2004-07-21

申请人： William Hamilton ,  Koen Hellendoorn ,  Timothy Jones ,  Dwayne Kirk ,  Hugh Mason ,  Xiuren Zhang ,  Charles Arntzen

发明人： William Hamilton ,  Koen Hellendoorn ,  Timothy Jones ,  Dwayne Kirk ,  Hugh Mason ,  Xiuren Zhang ,  Charles Arntzen

IPC分类号： A01H1/00 ,  A61K20060101 ,  A61K39/12 ,  C12N7/00 ,  C12N15/82 ,  C12Q1/70

CPC分类号： C12N15/8258 ,  A61K2039/5258 ,  A61K2039/542 ,  C07K14/005 ,  C12N2770/16022

摘要： The present invention relates to a synthetic plant-optimized nucleic acid molecule having a Norwalk virus capsid protein coding nucleotide sequence, and nucleic acid constructs, host cells, expression systems, and plants having the plant-optimized Norwalk virus nucleic acid molecule. The present invention also relates to a method of producing Norwalk virus capsid protein virus-like particles in a transgenic plant or transgenic plant seed transformed with a plant-optimized nucleic acid molecule encoding Norwalk virus capsid protein. The plant or a component thereof can be administered to a subject under conditions effective to immunize the subject against disease resulting from infection by a Norovirus, including Norwalk virus. An oral vaccine for immunization of a subject against Norwalk virus infection is also disclosed.

摘要翻译： 本发明涉及具有Norwalk病毒衣壳蛋白编码核苷酸序列的合成植物优化核酸分子，以及具有植物优化诺瓦克病毒核酸分子的核酸构建体，宿主细胞，表达系统和植物。 本发明还涉及在用植物优化的编码诺瓦克病毒衣壳蛋白的核酸分子转化的转基因植物或转基因植物种子中产生诺瓦克病毒衣壳蛋白病毒样颗粒的方法。 植物或其组分可以在有效免疫受试者的病症下施用于受诺如病毒（包括诺瓦克病毒）感染引起的疾病的病症。 还公开了用于免疫Norwalk病毒感染的受试者的口服疫苗。

公开(公告)号：US07879338B2

公开(公告)日：2011-02-01

申请号：US10895791

申请日：2004-07-21

申请人： William D.O. Hamilton ,  Koen Hellendoorn ,  Timothy D. Jones ,  Dwayne D. Kirk ,  Hugh S. Mason ,  Xiuren Zhang ,  Charles J. Arntzen

发明人： William D.O. Hamilton ,  Koen Hellendoorn ,  Timothy D. Jones ,  Dwayne D. Kirk ,  Hugh S. Mason ,  Xiuren Zhang ,  Charles J. Arntzen

IPC分类号： C12N15/40 ,  C12N5/04 ,  C12N1/21 ,  A61K39/125 ,  A01H5/00 ,  A01H5/08 ,  A01H5/10

CPC分类号： C12N15/8258 ,  A61K2039/5258 ,  A61K2039/542 ,  C07K14/005 ,  C12N2770/16022

摘要： The present invention relates to a synthetic plant-optimized nucleic acid molecule having a Norwalk virus capsid protein coding nucleotide sequence, and nucleic acid constructs, host cells, expression systems, and plants having the plant-optimized Norwalk virus nucleic acid molecule. The present invention also relates to a method of producing Norwalk virus capsid protein virus-like particles in a transgenic plant or transgenic plant seed transformed with a plant-optimized nucleic acid molecule encoding Norwalk virus capsid protein. The plant or a component thereof can be administered to a subject under conditions effective to immunize the subject against disease resulting from infection by a Norovirus, including Norwalk virus. An oral vaccine for immunization of a subject against Norwalk virus infection is also disclosed.

摘要翻译： 本发明涉及具有Norwalk病毒衣壳蛋白编码核苷酸序列的合成植物优化核酸分子，以及具有植物优化诺瓦克病毒核酸分子的核酸构建体，宿主细胞，表达系统和植物。 本发明还涉及在用植物优化的编码诺瓦克病毒衣壳蛋白的核酸分子转化的转基因植物或转基因植物种子中产生诺瓦克病毒衣壳蛋白病毒样颗粒的方法。 植物或其组分可以在有效免疫受试者的病症下施用于受诺如病毒（包括诺瓦克病毒）感染引起的疾病的病症。 还公开了用于免疫Norwalk病毒感染的受试者的口服疫苗。

公开(公告)号：US08975471B2

公开(公告)日：2015-03-10

申请号：US11247587

申请日：2005-10-12

申请人： Jose L. Reyes Taboada ,  Xiuren Zhang ,  Takashi Soyano ,  Nam-Hai Chua ,  Qi-Wen Niu ,  Shih-Shun Lin

发明人： Jose L. Reyes Taboada ,  Xiuren Zhang ,  Takashi Soyano ,  Nam-Hai Chua ,  Qi-Wen Niu ,  Shih-Shun Lin

IPC分类号： C12N15/82 ,  C12N15/113 ,  A01H5/00

CPC分类号： C12N15/113 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8261 ,  C12N15/827 ,  C12N15/8283 ,  C12N2310/141 ,  Y02A40/146

摘要： The invention provides methods and compositions useful in target sequence suppression, target sequence validation and target sequence down regulation. The invention provides polynucleotide constructs useful for gene silencing or RNA down regulation, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence or to down regulate RNA.

摘要翻译： 本发明提供了可用于靶序列抑制，靶序列验证和靶序列下调的方法和组合。 本发明提供了可用于基因沉默或RNA下调的多核苷酸构建体，以及包含多核苷酸的细胞，植物和种子。 本发明还提供了使用微RNA沉默靶序列或下调RNA的方法。

公开(公告)号：US20060130176A1

公开(公告)日：2006-06-15

申请号：US11247587

申请日：2005-10-12

申请人： Jose Reyes-Taboada ,  Xiuren Zhang ,  Takashi Soyano ,  Nam-Hai Chua ,  Qi-Wen Niu ,  Shih-Shun Lin

发明人： Jose Reyes-Taboada ,  Xiuren Zhang ,  Takashi Soyano ,  Nam-Hai Chua ,  Qi-Wen Niu ,  Shih-Shun Lin

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/113 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8261 ,  C12N15/827 ,  C12N15/8283 ,  C12N2310/141 ,  Y02A40/146

摘要： The invention provides methods and compositions useful in target sequence suppression, target sequence validation and target sequence down regulation. The invention provides polynucleotide constructs useful for gene silencing or RNA down regulation, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence or to down regulate RNA.