公开(公告)号：US10920251B2

公开(公告)日：2021-02-16

申请号：US16098914

申请日：2017-05-05

申请人： William Marsh Rice University

发明人： Ka-Yiu San ,  Zhilin Li ,  Xian Zhang

IPC分类号： C12N1/20 ,  C12P7/64 ,  C12N9/02 ,  C12N15/52 ,  C12N9/16 ,  C12N9/88 ,  C12N9/04 ,  C12N15/74

摘要： This invention describes a method of using microbial to produce fats, such as fatty acids and their derivatives, or products derived from the fatty acid synthesis cycle, such as hydroxyfatty acids, methyl ketones, and the like.

公开(公告)号：US10851393B2

公开(公告)日：2020-12-01

申请号：US15553129

申请日：2016-02-22

申请人： WILLIAM MARSH RICE UNIVERSITY

发明人： Ka-Yiu San ,  Xian Zhang ,  Hui Wu ,  Dan Wang

IPC分类号： C12N1/21 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/16 ,  C12P7/64 ,  C12N1/20

摘要： The present disclosure describes a genetically engineered a KASIII-independent fatty acid biosynthetic pathway that makes use of the promiscuous nature of the rest of the FAS enzymes (3-ketoacyl-ACP synthetase, 3-ketoacyl-ACP reductase, 3-hydroxyacyl ACP dehydrase, enoyl-ACP reductase) to bypass the KASIII step by providing a Co-A precursor of two or higher than two carbons (such as the four carbon butyryl-CoA) as the starting molecule. Since many CoA-based starter molecules can be supplied for the fatty acid synthesis, much more diversified products can be obtained with various carbon-chain lengths. As such, this disclosure will serve as a powerful and efficient platform to produce low to medium chain length products carrying many different functional groups.