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公开(公告)号：US10920251B2
公开(公告)日：2021-02-16
申请号：US16098914
申请日：2017-05-05
发明人： Ka-Yiu San , Zhilin Li , Xian Zhang
摘要： This invention describes a method of using microbial to produce fats, such as fatty acids and their derivatives, or products derived from the fatty acid synthesis cycle, such as hydroxyfatty acids, methyl ketones, and the like.
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公开(公告)号：US10851393B2
公开(公告)日：2020-12-01
申请号：US15553129
申请日：2016-02-22
发明人： Ka-Yiu San , Xian Zhang , Hui Wu , Dan Wang
摘要： The present disclosure describes a genetically engineered a KASIII-independent fatty acid biosynthetic pathway that makes use of the promiscuous nature of the rest of the FAS enzymes (3-ketoacyl-ACP synthetase, 3-ketoacyl-ACP reductase, 3-hydroxyacyl ACP dehydrase, enoyl-ACP reductase) to bypass the KASIII step by providing a Co-A precursor of two or higher than two carbons (such as the four carbon butyryl-CoA) as the starting molecule. Since many CoA-based starter molecules can be supplied for the fatty acid synthesis, much more diversified products can be obtained with various carbon-chain lengths. As such, this disclosure will serve as a powerful and efficient platform to produce low to medium chain length products carrying many different functional groups.
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公开(公告)号：US10465212B2
公开(公告)日：2019-11-05
申请号：US15073641
申请日：2016-03-18
发明人： Ka-Yiu San , Mai Li , Xiujun Zhang
摘要： There is provided a recombinant bacterium comprising at least one overexpressed acyl-ACP thioesterase gene, and wherein at least one gene from the tricarboxylic acid cycle or glycolysis or both is inactivated. There is also provided a method for producing fatty acids, said method comprising culturing bacteria comprising at least one overexpressed acyl-ACP thioesterase gene in a growth medium in a container having walls; allowing said bacteria to secrete fatty acids; and collecting said fatty acids. Acid supplementation is also shown to increase productivity.
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公开(公告)号：US09598696B2
公开(公告)日：2017-03-21
申请号：US14350544
申请日：2012-10-16
发明人： Ka-Yiu San , Mai Li
IPC分类号： C12N1/20 , C12N15/00 , C12N9/16 , C12N9/00 , C12Q1/68 , C12P7/64 , C07H21/04 , C12N15/70 , C07K14/24 , C12N15/52
CPC分类号： C12N15/70 , C07K14/24 , C12N9/16 , C12N15/52 , C12P7/6409 , C12Y301/02014 , Y02P20/52
摘要： The present invention discloses a process for increasing the production of free fatty acids at high yield (close to maximum theoretical yield), with various fatty acid compositions and various percentage of fatty acids accumulated intracellularly. This invention will enable the efficient production of other products derived from free fatty acids and/or products that can be branched out from the fatty acid synthesis pathways.
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公开(公告)号：US10752925B2
公开(公告)日：2020-08-25
申请号：US16015101
申请日：2018-06-21
发明人： Ka-Yiu San , George N. Bennett , Irene Martinez
摘要： Microbes and methods used to convert renewable carbon sources such as glucose, sucrose, biomass hydrolysate, methanol or formate, to succinate-derived products, such as fumarate or malate, which are desirable products having many uses.
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公开(公告)号：US20140273114A1
公开(公告)日：2014-09-18
申请号：US14350544
申请日：2012-10-16
发明人： Ka-Yiu San , Mai Li
CPC分类号： C12N15/70 , C07K14/24 , C12N9/16 , C12N15/52 , C12P7/6409 , C12Y301/02014 , Y02P20/52
摘要： The present invention discloses a process for increasing the production of free fatty acids at high yield (close to maximum theoretical yield), with various fatty acid compositions and various percentage of fatty acids accumulated intracellularly. This invention will enable the efficient production of other products derived from free fatty acids and/or products that can be branched out from the fatty acid synthesis pathways.
摘要翻译： 本发明公开了一种以高收率（接近最大理论产率）增加游离脂肪酸生产的方法，其中各种脂肪酸组合物和各种百分比的脂肪酸在细胞内积累。 本发明将能够有效地生产衍生自可从脂肪酸合成途径中分出的游离脂肪酸和/或产物的其它产物。
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公开(公告)号：US10774349B2
公开(公告)日：2020-09-15
申请号：US15572099
申请日：2016-05-07
发明人： Ka-Yiu San , Dan Wang
摘要： The present disclosure describes an engineered microorganism for producing alpha omega bifunctional C6-16 fatty acids from renewable carbon sources.
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公开(公告)号：US10011854B2
公开(公告)日：2018-07-03
申请号：US15095158
申请日：2016-04-11
发明人： Ka-Yiu San , Wei Li
CPC分类号： C12P7/6409 , C12N9/0006 , C12N9/16 , C12Y101/01 , C12Y101/011 , C12Y101/0133 , C12Y301/02014
摘要： The present disclosure relates to an engineered microbe capable of improved productivity of fatty acid or fatty acid derivative. An NAD+-dependent 3-oxoacyl-ACP reductase or NAD+-dependent 3-oxoacyl-CoA reductase replaces or supplements the native NADP+-dependent 3-oxoacyl-ACP reductase so as to utilize the higher availability of NAD+ rather than NADP+ in the cell. Higher production, yield and titer of fatty acids are therefore obtained. Such microbes can be combined with other mutations to further improve yield of fatty acids or fatty acid derivatives.
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公开(公告)号：US09388434B2
公开(公告)日：2016-07-12
申请号：US14161456
申请日：2014-01-22
发明人： Ka-Yiu San , Songi Han
CPC分类号： C12P7/6409 , C12N9/16 , C12Y301/02014 , Y02P20/52
摘要： The present invention relates to an engineered bacteria for producing short chain fatty acid with the overexpression of a long chain (>C12) acyl-ACP thioesterases (long-TE) and a short chain (≦C12) acyl-ACP thioesterases (short-TE).
摘要翻译： 本发明涉及用于生产短链脂肪酸的工程细菌，其具有长链（> C12）酰基-ACP硫酯酶（长TE）和短链（＆amp; C12E）C12酰基-ACP硫酯酶（短链） TE）。
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公开(公告)号：US11174468B2
公开(公告)日：2021-11-16
申请号：US16092042
申请日：2017-04-06
发明人： Ka-Yiu San , Ping Liu , Sha Li
摘要： The present disclosure describes a genetically engineered bacteria that relieves the catabolite repression problem exerted by the Spot 42 small regulatory RNA by adding a galactokinase that does not contain the Spot 42 binding region. As such, galK and galM and the like can be expressed allow better galactose utilization.
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