公开(公告)号：US5633441A

公开(公告)日：1997-05-27

申请号：US361467

申请日：1994-12-22

申请人： Willy De Greef ,  John Van Emmelo ,  Dulce E. De Oliveira ,  Maria-Helena De Souza ,  Marc Van Montagu

发明人： Willy De Greef ,  John Van Emmelo ,  Dulce E. De Oliveira ,  Maria-Helena De Souza ,  Marc Van Montagu

IPC分类号： C07K14/415 ,  C12N9/10 ,  C12N9/22 ,  C12N9/50 ,  C12N15/55 ,  C12N15/62 ,  C12N15/82 ,  A01H5/00 ,  C12N15/11

CPC分类号： C12N9/1007 ,  C07K14/415 ,  C12N15/62 ,  C12N15/829 ,  C12N9/22 ,  C12N9/50 ,  C07K2319/00 ,  C07K2319/02 ,  Y10S47/01

摘要： The invention concerns female-sterile plants that comprise a foreign DNA incorporated in the nuclear genome of their cells. This foreign DNA first comprises a female-sterility DNA encoding a protein or polypeptide such as barnase which, when produced in the cells of the plant, kills or significantly disturbs the metabolism, functioning or development of the cells. The foreign DNA also comprises a first promoter which directs expression of the female-sterility DNA selectively in style cells, stigma cells or style-and stigma cells of the female reproductive organs of the plants. The first promoter does not direct detectable expression of the female sterility DNA in the ovule or in other parts of the plant so that the plant remains male-fertile. The female-sterility DNA is in the same transcriptional unit as and under the control of the first promoter.

摘要翻译： 本发明涉及包含掺入其细胞的核基因组中的外来DNA的雌性不育植物。 该外源DNA首先包含编码蛋白质或多肽（如芽孢杆菌RNA酶）的雌性不育DNA，当在植物细胞中产生时，它们杀死或显着地干扰细胞的代谢，功能或发育。 外源DNA还包含第一启动子，其指导雌性不育DNA在植物的雌性生殖器官的样式细胞，柱头细胞或样式和柱头细胞中的表达。 第一个启动子不能引导雌性不育DNA在胚珠或植物的其他部分中的可检测的表达，使得植物保持雄性可育。 雌性不育DNA与第一启动子处于相同的转录单位和控制下。